Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
THIRD SEMESTER – NOVEMBER 2013
BT 3825 - BIOPROCESS & PHARMACEUTICAL BIOTECHNOLOGY
Date : 12/11/2013
Time : 9:00 - 12:00
Dept. No.
Max. : 100 Marks
PART- A
I Choose the correct answer:
(20marks)
Answer all questions
1. Pyrococcus abyssi is an example of
a) Thermophiles
b) Hyperthermophiles
c) Mesophiles
(5 x 1 = 5)
d) Psychrophiles
2. ____________ phase is prolonged by the addition of fresh medium to the vessel.
a) Log
b) Lag
c) Stationary
d) Decline
3. Vitamin B12 is an example of _____________.
a) Intra cellular metabolites
c) Both a and b
b) Extra cellular metabolites
d) None of the above
4. ______________ is used in toothpaste.
a) Sanguinarine
b) Quinine
c) Caffeine
d) Morphine
5. IND refers to
a) International New Drug
c) Indian New Drug
b) Investigational New Drug
d) Investigational Nano Drug
II State whether the following are True or False; if false, give reason:
(5 x 1 = 5)
6. Polytetrafluoro ethylene is used in depth filters.
7. Precursors are chemicals directly incorporated with products.
8. Glucanase enzyme is used to disrupt yeast cells.
9. Conformational epitopes are formed by continuous sequence of amino acids in a protein.
10. Oral corticosteroids are also used in conditions of autoimmunity.
III Complete the following:
(5 x 1 = 5)
11. In a fermenter ____________ is used for mixing microorganisms and nutrients.
12. The storage of fungi and actinomycetes is done by ____________ culture technique.
13. Alcohol used as motor fuel is referred to as _____________.
14. _____________ is an antidote to nerve gas poisoning.
15. The __________ portion of rituximab mediates antibody-dependent cellular cytotoxicity.
IV Answer the following each within 50 words only
(5 x 1 = 5)
16. Define Osmosis.
17. What is an Inhibitor?
18. What is homogenized milk?
19. Mention the subtypes of coumarins.
20. Write the types of phase I clinical trials.
PART- B
Answer the following, each within 500 words;
Draw diagrams wherever necessary:
21. (a) Give an account on absolute filters.
(b) Write short note on
(5 x 8 = 40 marks)
OR
ii) Temperature
i) Radiation
22. (a) Discuss the energy sources used in media formulation.
OR
(b) Enumerate the role of mutation in strain improvement.
23. (a) Describe the production of SCP from algae with an example.
OR
(b) Write about the production of glutamic acid.
24. (a) Write short note on
i) Peptide mapping
OR
(b) Describe the effects and functions of prostaglandins.
ii) RP-HPLC
25. (a) Explain the production of monoclonal antibodies.
OR
(b) Write the properties, synthesis and detection of Quantum Dots.
PART - C
Answer any two of the following, each within 1500 words;
Draw diagrams wherever necessary:
26. Write short notes on:
(i) Bubble column bioreactor
(iii) Packed bed bioreactor
(2x 20 = 40 marks)
(ii) Air lift bioreactor
(iv) Continuous stirred tank bioreactor
27. Describe the process of developing an inoculum for industrial use with any two examples.
28. What is downstream processing? Explain the different stages in it.
29. Enumerate the role of flavonoids in the treatment of rheumatoid arthritis and cancer.
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