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BIOLOGY 263, FALL 2002
MIDTERM EXAMINATION
Name
Date:
Laboratory Practical Stations. Answer the questions at each station. (14%, 4% per station)
1. a.
b.
2. a.
3. a.
b.
4. Complete the activity as directed.
b.
Hypothesis Evaluation. Is there statistically significant support for each of the following
hypotheses? (6%, 2% each)
1. Hypothesis = The number of bird species will be greater in wooded areas than in grassland areas in
eastern Colorado.
Data = Mean number of bird species sighted per 10 meter transect in wooded areas = 12.4, Mean
number of bird species sighted per 10 meter transect in grassland areas = 16.8.
p-value = 0.0007
Circle one: Hypothesis Supported
Hypothesis NOT Supported
2. Hypothesis = Common mallow, Malva neglecta, is more common in lawns than in empty lots.
Data = Mean # of Malva neglecta plants per m2 in lawn = 19.5, Mean # of Malva neglecta plants per
m2 in empty lots = 19.2
p-value = 0.456
Circle one: Hypothesis Supported
Hypothesis NOT Supported
3. Hypothesis = There are more microvinvertebrate species in wooded areas than in grassland areas in
eastern Colorado.
Data = Mean # of invertebrate species per quadrat sample in wooded areas = 10.4, Mean # of
invertebrate species per quadrat sample in grassland areas = 9.1
p-value = 0.048
Circle one: Hypothesis Supported
Hypothesis NOT Supported
Laboratory Safety & Behavior Scenario. Read the scenario below and identify four things that
Herb did incorrectly or unsafely and BRIEFLY explain why these four things were incorrect or
unsafe. (10%)
Herb walks into lab wearing athletic shoes and drinking Gatorade from a sports bottle. He sits down and
begins to work on the prokaryote laboratory with his laboratory partner. In collecting materials he lays a
broth culture of Escherichia coli down on his lab bench. The culture begins to leak through its cap all
over the bench. Herb reacts quickly and cleans the spill up himself using 95% ethanol and Kimwipes
before anyone else noticed. He disposes of the used Kimwipes in the Biohazard waste. After that incident
Herb is very careful in doing his Gram staining, making sure that the solutions coming off of the slide
drip into the sink where they can be easily washed away.
Definitions. Define each BIOLOGICAL term from THIS LABORATORY in an accurate,
concise, and lucid manner. (20%, 4% each)
1. Bacterial Colony:
2. Electrophoresis:
3. Forb:
4. Negative Control Group:
5. p-value:
Data Manipulation & Analysis. Address each question in as concise and lucid a manner as
possible.
1. Given the following data from a small, reproductively isolated, constant-sized population,
a) calculate the allele and genotype frequencies for every time period. (8%)
b) Create a properly labeled graph of the allele frequency of one of the alleles (NOT both)
over time. (5%)
c) Propose a plausible population genetic/evolutionary explanation for what you see happening
(or not happening) to the allele frequency over time. (2%)
generations
0
5
10
15
20
individuals
BB
3
6
4
2
4
Bb
6
4
6
5
6
bb
3
2
2
5
2
2. Use the following four gels on which are shown X and x alleles (all individuals are from the
same population) to calculate the allele and genotype frequencies for the population. Then
determine if the population is at Hardy-Weinberg equilibrium. (10%)
wells
x
X
2. Use the following data table to construct a phylogenetic tree. Be certain to illustrate each
derived characteristic on the tree. (10%)
mobile
stage
fruit
wood
leaves
root
branching
Pine
seeds
no fruit
woody
needle
irregular
Apple
seeds
fruit
woody
flat
irregular
Pear
seeds
fruit
woody
flat
irregular
Spruce
seeds
no fruit
woody
needle
irregular
Fern
spores
no fruit
herbaceous
flat
irregular
Club
moss –
Outgroup
spores
no fruit
herbaceous
flat
dichotomous
Experimental Design. Use the back of this page to design a rigorous experiment with
appropriate control(s) to test the hypothesis that Streptomyces griseus ( a soil living bacterium)
inhibits growth of Micrococcus luteus ( another soil living bacterium). (13%) Create a scientific
title for the laboratory report you would write for this experiment. (2%)
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