Download Document 8885391

GOOLWATER TRIM TM
SuperCitriMax@
75 DAY IPRE-MARKET' N01fIFICA7IUN
APPENDIX f.: : MECHANISMS OF ACTION
Overview
in this Appendix there are 3 aiticies These articles provide a basic, understanding of the
mechanism of 1oHf HCA works.
The first article is by John Lotirenstein of Brandeis University who was ore of the key figures
in Yhe early research conducted on HCA. The article entitled "Experiments with
F-iydroxycitrate" is 3n overview of much of his work . His important conclusion is that the
citrate cleavage pathway is the major source of carbon for the synthesis of fatty acid in ri:,t.
Proving that hypothesis was made possible by using the knowledge that (+hydroxycitratp is
an inhibitorof citrate cleavage enzyme
The second article entitled "Tricarballylate and Hyr,lroxycitrate : Substrate and Inhibitor of ATp
C'itrate Oxaloac:etate Lyase" is ; an investigation into the activity of the citrate cleavage
enzyme . The si :ud~~ was also conducted by Lowen :stein and his colleagues at Brarcleis
Lsriversity . !i provides evidence Via1r the (-)-isomer of hyckroxycitrate is indeed an inhibitor of
citrate cleavage enzyme
The third study presented in this Appendix was led by Harry PreuSs of Georgetown University
Medical Centre and is an investigation of the effects of a natural extract of (-)-hydroxycitrir.
acir alone and in combination with other natural ingredients an weight loss . The study was
conducted under controlled circumstances on 60 moderately obese subjects . The findings
afte ; 8 weeks of HCA supplemertation were that appetite, Body Mass Index, total cholesterol,
low- density lipoproteins, triglyrerides and serum le:ptin levels were significantly reduced, while
high density lip0protein levels and excretion of urinary fat metabolites had increased . In effect
HC,? was shown to be a useful aid in a diet regime while ii: also contributed to healthy blood
lip,c profiles .
These three aniclEs are representative of many other Studies that have been conducted i)n
the
ef ects of HCA .
INDEX
Page
Page!
116
Experiments With Q-)-Hydiroxycil:rate
John M I_owenstein
Tricarballylsite And Hydroxyc;itrate: Substrate And Inhibitor Of Atp l:itraF;e
Oxaloacetale Lyase
John A Watson, Mairie Fang ard John M Lowenszein
130
140
Page
Effects o~f a natural extract of (-)-hydroxycitric acid (HC:a:5X) and a combination of H( :ja,SX pl3is
niacin-bound chromium and Gymnema sylvestre extract an weight loss
H G. Pr,~~us<.,1 D 9agchi,2 M 9agchi .2 G ~' . S Rao,3 D K. Dey4 and 5. Satyanarayana 5
SHANNON MINERALS LID
Page 1115