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University Instrumentation Center TRANSMISSION ELECTRON MICROSCOPE INSTRUMENT OVERVIEW The transmission electron microscope (TEM) uses a beam of electrons transmitted through an ultra-thin specimen. Using a beam of electrons rather than the photons of a light microscope, allows greater resolution and enables researchers to study ultrastructure of organelles, viruses and macromolecules. Formed from the interaction of the electrons transmitted through the specimen; the resulting image is magnified and focused onto a digital display. Since electrons are easily deflected by gas molecules, a vacuum environment is necessary for the TEM system, electron beam and specimen. The TEM can also be used to characterize the microstructure of materials with very high spatial resolution. Information about the morphology, crystal structure and defects, crystal phases and composition can be obtained. APPLICATIONS • • • Sunflower leaf at 800X magnification. Analysis of physical and biological scientific fields for a variety of applications such as cancer research, virology, material science, pollution research, and nanotechnologies Imaging of magnetic nanoparticles to target drug delivery in cancer therapy Research support of polymer-based paints, coatings, adhesives, and sealants INSTRUMENT SPECIFICATIONS • • • • Zeiss/LEO 922 Omega Transmission Electron Microscope (TEM) Accelerating voltage 120 KV Resolution line of 0.2 nm Sample size – Diameter = 3 mm max T: 603-862-2790 | F: 603-862-4104 | [email protected] | www.unh.edu/uic W123 Parsons Hall, 23 Academic Way, Durham, NH 03824 © 2014 University of New Hampshire. All Rights Reserved. “Poly(methyl methacrylate) homoarm star polymers taken on 400 mesh Formvar film coated 400 square mesh copper grids. Samples were drop-casted using a 1 mg/mL solution in THF.” Follow us on Twitter @UNHInstruments