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Free DNA Receptor for DNA Chromosome bacteria Two strains of Acinetobacter calcoaceticus: One strain is resistant to streptomycin – resistant strain One strain is not resistant to streptomycin – sensitive strain 1 hour @ 60°C Live Resistant Strain Lysing solution + Live Resistant strain Lysing solution + Killed Resistant strain Tryptic soy agar Live Sensitive Live Resistant A B C S R S+ KR D KR S = Sensitive Killed Resistant R = Resistant K = Killed Resistant Week one growth check: A B C D KR S Yes R Yes S+ KR No Yes Bacteria were then transferred to a plate containing TSA with Streptomycin: Plate contains TSA and Streptomycin: S+ KR S A R B C Week two growth check: Yes No S Yes A R S+ KR C B Conclusions: 1) Lysing did kill the resistant strain 2) The sensitive strain was sensitive to streptomycin 3) Genetic transformation did occur
