Download Biotech

Biotech
Cloning a mammal
PCR
• This is the polymerase chain reaction. It is a
technique to multiply a sample of DNA many
times in a short period of time. It supplies the
scientist with sufficient DNA for further testing.
http://www.dnalc.org/resources/animations/pcr.html
DNA fingerprinting (RFLP analysis)
• RFLP is restriction fragment length
polymorphism. This is the idea that each
person’s DNA would cut up into different
sized fragments. Thus the pattern
produced by electrophoresis of those
patterns would be unique to the person.
PROBE
• Why? To “light up/identify” specific genes
• Hybridization: complementary DNA binds
to a gene and holds another molecule to
“light up/identify” a specific sequence of
DNA.
• Often probe is radioactive or fluorescent
so when exposed to film it can be
detected.
Southern Blotting
• Through a series of steps, DNA that has
been separated by electrophoresis is applied
to a membrane of nylon or nitrocellulose.
DNA fingerprinting analysis
• http://www.biology.arizona.edu/human_Bio
/problem_sets/DNA_forensics_1/02Q.html
• Do problems 2-4 to practice the
interpretation of DNA fingerprints.