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DNA & RNA Standard 5C #19 Genetic Engineering & Biotechnology 5c. Know that genetic engineering (biotechnology) is used to produce novel biomedical and agricultural products. Genetic Engineering is a form of selective breeding. Selective Breeding allows only those animals or plants that have desired traits to reproduce. For thousands of years breeders have been selectively breeding dogs that may be better hunters, retrievers or companions. Luther Burbank used a method called HYBRIDIZATION to cross dissimilar daisies to bring together the best of both organisms. This is kind of like our parents. Dad is great at something and mom is great at another. Hopefully, their offspring are great at two or more things. X Insect Resistant Hybridization The crossing of the two plants above give us an insect resistant and great smelling rose. Great Scent For thousands of years people have been selectively breeding plants that produce better tasting fruit or plants that my be unaffected by insects, fungus,disease or other plant destroying pest. Burbank’s best work with plants allowed him to cross disease resistant plants with plants that had high food yields. The result were plants that allowed farmers to increase food supplies. Here scientist show off their very large corn plants that yield lots of corn. This corn is also more resistant to the fungus that affect most corn plants. Inbreeding- Is a technique used by breeders, to maintain desired characteristics. Here breeders allow individuals with similar characteristics to reproduce. Once a desired trait is obtained breeders try to maintain that trait. Despite man’s desire to manipulate plant or animal traits. Scientist have learned that mutations are the ultimate source of genetic variation. Biotechnology has allowed man to transform cells to find greater variation and ultimately a better organism. Scientist induced mutations in bacteria to produce bacteria that eat oil. Transgenic Microorganism reproduce rapidly and are easy to grow. Bacteria are used to make insulin, growth hormone and clotting factors that were all once rare and expensive. This is done by transforming cells by inserting a human gene into the cell of a bacterium. Remember: Way to high joke. When transformation is successful, the foreign DNA will be integrated into one of the cell’s chromosomes. Transforming Cells The ends of the Recombinant DNA recombine with sequences in the host cell DNA. Transforming Cells When the Recombinant DNA is inserted into the the target location, the host cell’s original gene is lost or knocked out of its place. Here a transgenic tobacco plant was created by inserting a gene from a fire fly into a tobacco cell. Transgenic cows are grown with extra copies of growth hormone genes. These cows grow faster and produce meat with less fat. Transgenic plants and animals have played a major role in increasing and improving our food supply. Cloning A clone is a member of a population of genetically identical cells produced by a single cell. Swedish scientist Ian Wilmut stunned the world, in 1997, when he successfully clone a sheep.This is how “Dolly” was cloned. 1. Scientist were able to produce oil-eating bacteria by? A. Inbreeding bacteria. B. Hybridizing bacteria. C. Inducing mutations in bacteria D.Making cell’s polyploidy. 2. What is the advantage of producing transgenic plants? A. Using more pesticides B. Producing clones C. Studying human genes D. increasing food supply 3. To make a new line of plants, Burbank used a process called? A. Inbreeding B. Cloning X C. Hybridization Insect Resistant Great Scent Hybridization D. Transformation The crossing of the two plants above give us an insect resistant and great smelling rose. 4. Which of the following is a clone? A. The adult sheep whose DNA was used to make dolly. B. A transgenic mouse. C. A funny person. D. Dolly 5. Why is Dolly considered to be a clone? A. The DNA in her cells are identical. B. She has a funny nose. C. She is genetically the same as her offspring. D. Her DNA source is a single body cell.