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αLA
Coomassie
E1 αKGDH
E2 PDHC
LipS
E3 αKGDH
and PDHC
E2 αKGDH
LipS in vitro activity.
Recombinant LipS was purified
and assayed in the presence of
protein substrate and cofactor Sadenosylmethionine (SAM). The
product of SAM cleavage (5’ dA)
was monitored over the course of
LipS reaction.
5' dA Produced by LipS
H Protein
70
Malaria LipS binds to the 3 lipoate-dependent enzyme
complexes found in E. coli. The labeled bands were
identified by amino acid sequencing and/or anti-lipoate
Western blot. Tight LipS/substrate complexes will now be
used to stabilize LipS, and to characterize the mechanism
guiding substrate specificity.
5' dA (uM)
60
50
40
30
20
10
0
0
5
10
Time (min)
15