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World Allergy Organization Journal 2016, Volume 9 Suppl 1
A407
Immune-Modulatory Genomic Properties Differentiate Gut
Microbiotas of Infants with and without Eczema
Gaik Chin Yap4, Wen Tso Liu1, Seungdae Oh1, Pei Ying Hong2, Chiung
Hui Huang3,4, Marion Aw3,4, Lynette Shek3,4, Bee Wah Lee3,4
1
University of Illinois at Urbana-Champaign, USA; 2King Abdullah
University of Science and Technology, Saudi Arabia; 3National University
Health System, Singapore; 4National University of Singapore, Singapore
Correspondence: Gaik Chin Yap – National University Health System,
Singapore
World Allergy Organization Journal 2016, 9(Suppl 1):A407
Background: The gastrointestinal tract is the primary site of interaction between the host immune system and microorganisms.
Studies have suggested that selective microbial targets may influence the development of the allergic diseases. But the difference
in functional gene composition remains unknown. We aim to assess the structural and functional gene composition of stool
microbiota of infants with eczema and their matched (for age,
gender, mode of delivery, feeding) controls at the age of 1
month.
Methods: Twelve children with eczema and their controls were
selected from the placebo arm of a birth cohort of at-risk infants participating in a randomized double-blind trial on the protective effects
of supplemental probiotics in early life on allergic outcomes. The four
were caesarean delivery followed by formula feeding (eczema = 2
and healthy control = 2) and the eight were vaginal delivery followed
by partial breast feeding mixed with formula feeding (eczema = 4
and healthy control = 4). Bacterial genomic DNA were extracted from
fecal samples and prepared for Illumina Miseq and Hiseq sequencing.
Data analysis such as sequence quality check, contigs assembly and
gene annotation were carried out for the DNA sequences obtained
from Miseq and Hiseq sequencing.
Results: Phylogenetic analysis of metagenomic sequences revealed
that four phyla dominated both microbial communities: Proteobacteria (54% and 63% for healthy and eczema communities, respectively), Firmicutes (26% and 18%), Actinobacteria (13% and 8%),
Bacteroidetes (7% and 8%). Comparative metagenomic analysis
showed that immune-regulatory TCAAGCTTGA motifs were significantly enriched in healthy communities, many of which were
encoded by Bifidobacterium (38% of the total motifs in the healthy
communities). Draft genomes of five Bifidobacterium species (B.
longum, B. bifidum, B. breve, B. dentium, and B. pseudocatenulatum )
were recovered from metagenomic datasets. The B. longum BFN-1212 genome encoded more TCAAGCTTGA motifs (4.2 copies per 1
million genome sequence) than other Bifidobacterium genomes and
was significantly overrepresented (P < 0.05) in the healthy
communities.
Conclusions: Our results report distinct immune-modulatory genomic
properties of gut microbiotas in healthy infants as compared to children with eczema and provide new insights into potential roles of
gut microbiotas in affecting human immune homeostasis.
A408
The Effect of Medication in OSA Patients with Allergic Rhinitis
Young Seok Byun1, Sung Wan Kim1, Tae Kyung Koh1, Joong-Saeng Jo1,
Kun Hee Lee2, Chul Kwon1, Sung-Hwa Dong1
1
Kyung Hee Medical Center, South Korea; 2Kyung Hee University
Hospital at Gangdong
Correspondence: Young Seok Byun – Kyung Hee Medical Center,
South Korea
World Allergy Organization Journal 2016, 9(Suppl 1):A408
Objective
Allergic rhinitis occurs at 10-40% in the world’s population and it
cause runny nose, sneezing, itching and qualitative degraded sleep
disorder which are causing the failure of social life. Allergic rhinitis
can be associated with patient of obstructive sleep apnea. The aim
of this study was to investigate the impact of allergic rhinitis on obstructive sleep apnea and the change of the symptoms of obstructive
sleep apnea after allergic rhinitis treatment.
Page 126 of 206
Method
We examined patients who diagnosed with obstructive sleep apnea
using polysomnography in our sleep clinic. All the patient was examined by allergy skin testing and diagnosed with allergic rhinitis.
Patients were group of OSA with AR. We did Allergic rhinitis medical
treatment for 2 weeks before OSA treatment.
We did the survey of Visual analogue scale, Epworth Sleepiness Scale,
Chalder Fatigue Scale, Daily Hassles Scale, Conner-Davidson Resilience Scale, Rhinoconjunctivitis Quality of Life Questionnaire before
and after Allergic rhinitis treatment.
Results
OSA-AR group was 14 pateints. Age ranged from 17 to 66 years old
and mean age 27. prior treatment, OSA-AR group score was less than
average in VAS(sleep time, wake time), ESS, CFS, DHS, CD-RISC, RQLQ
examination and TNSS, which associated with rhinitis in RQLQ,
showed less than average except nasal obstruction in OSA-AR. VAS
(sleep time, wake time) and RQLQ in patients who underwent
2weeks medical treatment showed statistically significant difference
after treatment. (sleep time P = 0.001, wake time P = 0.000, RQLQ
P=.011) The average scores of ESS, CFS, DHS, CD-RISC, RQLQ, TNSS
was lower but no significant difference after treatment than before
treatment.
Conclusion
We could find out Allergic rhinitis treatment contributes to improve
subjective symptoms and QOL of patients in OSA-AR treatment
patients. But considering lower number of subject, further investigation should be performed.
A409
A Case of Generalized Pustular Psoriasis Mimicking Acute
Generalized Exanthematous Pustulosis
Myung Shin Kim1, Chansun Park2
1
Soonchunhyang University Gumi Hospital South Korea; 2Haeundae Paik
Hospital, Inje University College of Medicine
Correspondence: Myung Shin Kim – Soonchunhyang University Gumi
Hospital, South Korea
World Allergy Organization Journal 2016, 9(Suppl 1):A409
Differentiating between acute generalized exanthematous pustulosis
(AGEP) and generalized pustular psoriasis (GPP) can be extremely difficult, as both present as erythematous pustular eruptions. A 60-yearold woman presented at the emergency department with generalized erythematous pustular eruption, generalized edema and fever.
She had treated with drug eruption by herbal remedy in dermatologic department for 3 weeks. One week before visiting ED, she received dapsone and zaltoprofen from another hospital. Body
temperature measured as 38.7 °C. Blood neutrophil was 85.3 % of
total leukocytes. Based on the clinical and laboratory findings, she
was diagnosed as AGEP by dapsone or NSAIDs. All the medications
were discontinued. After treatment with systemic steroid for 3 days,
fever and skin lesions were improving. However, new pustular lesions
were developing immediately after the dose reduction of steroid and
rapidly aggravated. The results of skin biopsy were compatible with
GPP rather than AGEP. Acitretin was started and steroid was slowly
tapered. The pustular lesion and scaling had improved. We report a
patient with a pustular eruption initially diagnosed as AGEP, with the
diagnosis changed to GPP. This case highlights the importance of
differential diagnoses in order to treat the condition appropriately.
A410
Anaphylaxis Caused By Gummy Jelly Ingestion: A Case Report
Han Seok Cho, Min-Ju Kim, Min Ji Kim, Young Ok Park, Hye Yeong Lee,
Hee Seong Kim, Eun Lee, Hyun-Ju Cho, Jinho Yu, Soo-Jong Hong, Keum
Hee Hwang
Asan Medical Center, South Korea
Correspondence: Han Seok Cho – Asan Medical Center, South Korea
World Allergy Organization Journal 2016, 9(Suppl 1):A410
Gelatin allergy has been well described in type 1 hypersensitivity reactions to vaccines, but only a few case reports have been published
about anaphylaxis associated with food.