Download Investigation of antibacterial effects of Zizyphus jujuba

Investigation of antibacterial effects of
Zizyphus jujuba
P-02.08.5-022
H.I. OzkanI, Y. AticiII, T. YavuzIII, Y. ÖzelIV, A.A. HismiogullariI, Ö. YavuzI
IBalikesir University, Faculty of Medicine, Department of Medical Biochemistry, Balikesir,
Turkey, IIBalikesir University, Health Sciences Institute, Department of Veterinary
Biochemistry, Balikesir, Turkey, IIIBalikesir University, Faculty of Medicine, Department of
Medical Microbiology, Balikesir, Turkey, IVBalikesir University, Health Sciences Institute,
Department of Medical Microbiology, Balikesir, Turkey
Plants are most important parts of natural resources that alternatively referred to
synthetic drugs for reasons such as being less side effects and lower costs.
Ziziphus jujuba Miller (Z. jujuba), a plant used in traditional medicine, is one of
the most important Ziziphus species belonging to Rhamnacea family. The fruit and
seeds of this plant are used different purposes such as anti-inflammatory,
antioxidant, immune-stimulant and wound healer. In this study, we investigated the
antibacterial effects of Z. jujuba.
The aims of this study were to screen the antibacterial activity of Z. jujuba. The
extract was obtained from Z. jujuba fruits pulverized with the aid of ball mill using
50% aqueous-ethanol solution. Extracts were screened for antimicrobial activity
against six different standard strains of bacteria by determining minimum inhibitory
concentration (MIC) according to CLSI criteria. Serial dilutions are made between 64
mg/mL and 0.031 mg/mL concentration range. The lowest concentration of wells that no
visible growth has been accepted as MIC value. Materials in the MIC and
lower concentrated wells were transferred to 5% Sheep Blood Agar petri dishes for
calculation of minimal bactericidal concentration (MBC). The lowest concentration
that no colony formation has been accepted as MBC value.
jujuba showed the most potent effect on strain of S. aureus ATCC 29213 is Grampositive cocci (MIC: 2 mg/mL). The MIC values of other Gram-positive bacteria S.
aureus ATCC 43300, E. faecalis ATCC 51219, L. monocytogenes F 1483 and M. smegmatis
CMM 2067 were detected as 8, 16, 8 and 8 mg/mL respectively. MIC values of Gramnegative bacilli were detected as > 64 mg/mL.
Consequently, Z. jujuba was found to be effective on Gram-positive cocci bacteria (
S. aureus ATCC 29213, S. aureus ATCC 43300 and E. faecalis ATCC 51219). The strongest
effect was observed on S. aureus ATCC 29213 strain. In contrast, extract showed less
effect on Gram-negative bacilli.