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Adnan I. AL-Hindi , PhD
Islamic University of Gaza 2009-2008
31
A practical guide to Diagnostic Medical Parasitology
PROTOZOA
* Amoebae (Intestinal)
Endolimax nana
Pathogenic
Disease
Acquired
Body site
Symptoms
Clinical specimen
Epidemiology
Control
Diagnosis
General
comments
Description
No
None
Faecal-oral transmission; contaminated food and water
Intestine
None
Stool
Worldwide, primarily human-to-human transmission
Improved hygiene, adequate disposal of faecal waste,
adequate washing of caontaminated fruits and vegetables
The standard O&P examination is recommended for
recovery and identification of E. nana in stool specimens.
Microscopic examination of direct saline wet mount may
reveal small, motile trophozoites.
An asymptomatic individual may have few trophozoites and
possibly only cysts in the stool.
Although many people worldwide are infected with this
organism. E. nana is one of the smaller amoebae, and its
trophozoite stage and that of Iodamoeba bϋtschlii look very
similar.
Motility has been described as sluggish and nonprogressive with blunt, hyaline pseudopods, In the
permanent stained smear, the nucleus is easy to see. The
karyosome tend to be large. The trophozoites measure 6
to 12 µm, with a usual range of 8 to 10 µm. The
cytoplasm may have small vacuoles containing ingested
debris or bacteria.
Trophozoites discharge their undigested food and begin to
round up prior to precyst and cyst formation. Early cysts
may contain very thin, curved chormatoidal bars. Eventually
the nuclei divide until the mature cyst, containing four nuclei,
is formed.
Cysts usually measure 5 to 10 µm, with a normal range
of 6 to 8 µm.