Download Anti-IKKy/NEMO (NT) pAb

Anti-IKKγ / NEMO (NT)
CATALOG NO.:
PX138A
SIZE: 100 µg
PX138B
SIZE: 0.5 mg
Western blot analysis of IKKγ in
BACKGROUND:
HeLa whole cell lysate with
Nuclear factor kappa B (NF-κB) is a ubiquitous
anti-IKKγ/NEMO at 1 µg/ml.
transcription factor and an essential mediator of gene
expression during activation of immune and inflammatory
responses. NF-κB mediates the expression of a great
APPLICATION:
variety of genes in response to extracellular stimuli. NF-
This polyclonal antibody can be used for detection of
κB is associated with IκB proteins in the cell cytoplasm,
IKKγ by Western blot at 0.5 to 1 µg/ml. HeLa whole cell
which inhibit NF-κB activity. The IκB kinase (IKKα and
lysate can be used as positive control and a 52 kDa
IKKβ)
band should be detected. It is human, mouse, and rat
phosphorylates
IκB
and
mediates
NF-
κB activation. A novel molecule in the IKK complex was
reactive and has no cross response to IKKα or IKKβ.
recently identified and termed IKKγ/NEMO/FIP3/IKKAP1.
IKKγ interacts with IKKβ and is required for the activation
STORAGE:
of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1
It is supplied as immunoaffinity chromatography purified
stimulation. FIP3 was also shown to bind to RIP and NIK
IgG, in PBS containing 0.02% sodium azide. Store at
and to mediate TNF-induced NF-κB activation.
4˚C, stable for one year.
SOURCE:
Rabbit anti-IKKγ polyclonal antibody was raised against a
RELATED PRODUCTS:
peptide (CQYQAPDMDTLQIHVME) corresponding to
Blocking peptide, 50 µg at 200 µg/ml, is available for
amino acids 400 to 416 of human IKKγ, which are
competition studies (Catalog No. PX136BP).
identical to those of mouse homologue.
NOT FOR HUMAN USE. FOR RESEARCH ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
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