Download Work Plan and Methodology: A)Work Plan: B) Methodology:

Work Plan and Methodology:
A)Work Plan:
First Year:
Literature Review, collection of Sewage water, MFC setup. Qualitative
analysis of wastewater.
Second Year:
Standardization of MFC setup, monitoring of MFCs for power generation
statistical analysis .
B) Methodology:
I. Collection of Materials
An amount of Sewage wastewater was carefully collected using gloves and
was stored in a sterile, sealed container. A liter of wastewater was also collected and
stored
Design and set up of MFC
• Ten plastic bottles (400 ml capacity each)
• Zinc electrodes (length = 14.0 cm, diameter = 0.7cm)
• Carbon electrodes (length = 14.0 cm, diameter = 1.5cm)
• Copper wire was inserted in electrodes to connect the circuit.
• Multimeter (Univolt, DT-860, Japan.) was connected to measure the voltage.
• Chemicals Used:- Phosphate Buffer :- pH- 7.7 (0.2 M)., HCL- 50%
• Egg Membrane.
• PVC Pipe (Ordinary used pipe):- 7 cm long, 0.5 cm diameter.
• Micro-organism used: - Lactobacillus. (Used one day curd).
• Sewage wastewater.
II. Construction of Microbial Fuel Cell Setups
An MFC consists of 4 parts; the anode chamber, the cathode chamber, a
permeable membrane, and an electrical circuit.
i. Framework
Two plastic bottles were used as chambers of an MFC. Two holes were drilled
from the sides of the two plastic bottles such that a PVC pipe can be connected
rigidly. Each container was about 7 cm high and about 0.5 cm in diameter. Two holes
were also made on the lids of the bottles such that a copper wire can be passed
through
ii Membrane
Preparation of membrane was obtained using acid treatment.
iii. Circuit
Carbon rods and Zinc rods were taken f that served as the electrodes of the
microbial fuel cell. the carbon and Zinc rods have a length of 14 cm each . The carbon
rods have diameter 1.5cm and Zinc rods have diameter of 0.7cm, both rods put in a
beaker filled with water and were left to set for twenty-four (24) hours. In assembling
the circuit, these were the materials used: copper wires, carbon and Zinc rods, four (4)
alligator clips, multimeter/multitester (Univolt, DT-860, Japan.) . The black copper
wire was for the anode and the red copper wire was for the cathode. The black wire
was inserted to the hole in one bottle lid and was connected to a Zinc rod while the
other end is connected to multimeter using an alligator clip. The same procedure was
done to the red wire. The lids were sealed with acrylic.
iv. Phosphate buffer
Phosphate Buffer (0.2 M):- pH- 7.7
v. Anode and Cathode
The anode chamber was inoculated with 25ml one day fresh curd which reach
in Lactobacillus was dissolve in 340 ml of sewage water collected from local area of
Akurdi. The cathode chamber was filled with phosphate buffer (0.2 M) had pH 7.7.
No mediator was used in anodic chamber, during metabolism of organic waste in
anodic chamber by bacteria the acidic condition were takes places, during these
condition increasing voltage output were considered as stable operation condition of
MFC.