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Scheme of Studies for MS / MPhil / MPhil leading to PhD (Zoology) Two / Four Years Programme The courses under Annexure V are proposed for MPhil and PhD students of the Department of Zoology, Shaheed Benazir Bhutto University. Each MPhil and PhD student shall be required to complete Courses of 24 Credit hours, and 12 Credit hours of Research work. Students having MS/MPhil from HEC recognized Universities will be exempted from the 24 Credit hours of Course work, if admitted to PhD Programme. The subjects will be offered depending upon the expertise available in the University. Qualifying CGPA for research will be 3. The following courses are proposed for MPhil, MS and PhD Programme SEMESTER SEMESTER-1 Course No ZOOL-501 ZOOL-502 ZOOL-503 Title Molecular Genetics Economic and Medical Entomology Parasitology/ Applied fisheries SEMESTER-2 ZOOL-504 ZOOL-505 ZOOL-506 Integrated Pests Management Application of Biotechnology Conservation and Wildlife Management/ Toxicology Preparation and Submission of Synopsis SEMESTER-3 SEMESTER-4 Research Research, Thesis and Presentation Seminar Total credit hours ***************** Credit Hours 3-1 3-1 3-1 12 3-1 3-1 3-1 0-0 12 4 4 2 10 34 Detail of Courses MS / MPHIL / MPHIL LEADING TO PHD ZOOLOGY (Two/Four Years Programme) SEMESTER-1 Course No. ZOOL-501 ZOOL-502 ZOOL-503 Title Molecular Genetics Economic and Medical Entomology Parasitology/Applied fisheries Credit Hours 3-1 3-1 3-1 12 ZOOL-501 Molecular Genetics 3-1 Historical development and scope of molecular genetics; the concept of paired genes: Mendel’s findings, inheritance pattern of Mendelian genes in mono and dihybrid cross. Chromosomal theory of inheritance and the concept of paired genes, the Johnson’s concept of gene and genotype. The concept of genes in populations; (a) multiple alleles Blood Groups in man (b) polygenic inheritance, skin color in man. The molecular basis of genetics Structure of DNA, clarification of DNA as genetic material, The Griffith experiments, the experiments of Avery, Macleod and McCarty, the Hershey and Chase experiments, theories of DNA Replication; fine structure of gene; RNA slicing. DNA stability Viral Genetics Basic features of viruses, lytic and lysogenic replication of λ phage; gene organization of λ phage; linear and circular forms of λ DNA; general features of M13 phage; the attraction of M 13 as cloning vehicle, viruses as cloning vehicles for other organisms, general features of hepatitis B virus (HBV), λ and M13 viruses. Bacterial Genetics Structure of a bacterial cell, plasmids; size and copy number, different types of plasmids. Diversity in bacteria. Genetics of eukaryotes Introduction and cellular organization in eukaryotes, the nuclear genome, organization of nuclear genome, packaging of DNA into chromosomes, chromosome morphology, telomeres, the cell cycle organellar genetic systems, mitochondrial genomes of man. Recent developments in Genetics Introduction to the: stem cell technology, Bt-genes, RR genes, Genomics, proteomics, bioinformatics. Practicals Preparation and estimation of DNA and RNA, plasmid preparation, DNA restriction and gel electrophoresis Books Recommended 1. Dimmoock, N. J., A. J. Easton and K. N. Leppard. 2002. Introduction to modern virology, pp 177-185, 312-350 2. Brown, T. A.1990. Gene cloning; an introduction. Chapmann and Hall, London, Newyork, Tokyo, Melborne, Madras, pp 17-48 3. Klug, W. S., and M. R. Cummings. 2003. Concepts of Genetics. Pearson Education. 413-441 4. Brown, T. A. 1992. Genetics: a molecular approach. Chapmann and Hall, pp 219289 5. Karp, J. Cell and molecular biology, concepts and experiments, 2005. Jhon Wiley and Sons, INC. 6. Geoffrey M.C., Robert E.H. The cell: A molecular approach, 2007. Sinauer associates, INC ZOOL- 502 Economic and Medical Entomology 3-1 Biology and classification of Arthropods of medical and veterinary importance with special emphasis on class Insecta (Order Diptera , Siphonaptera, Phithiraptera, Dictyoptera, Coleoptera, Hymenoptera and Lepidoptera) and Class Arachnida (Order Acarinae, Aranae, Scorpionida). Life cycle; effects of bites, allergies, pathology and immunology, Arthropod as vectors Arthropods as parasites and bloodsuckers, Insects and hygiene. Forensic Entomology. Practicals Collection, preservation and identification of insects upto families (except for the identification upto species of a few pests of great economic importance), with the help of keys/literature Books Recommended 1. Atwal, A. S. (1984) Agricultural pests of India and South East Asia. Kalyani Publishers Delhi 2. Imms, A.D. (1970) A General Textbook of Entomology. 9th ed. Revised by O.W. 3. Metcalf, G. L. and Flint, W.P. (1980) Destructive and useful insects. Mc Graw Hill New York 4. Herms, W. E. and Janes, M. T. Medical Entomology. The Macmillan Co. NewYork 5. Pfadt, R. E. Fundamental of applied Entomology. The Macmillan Co. NewYork 6. De Bach, P. Biological control of insect pests and weeds. Chapman and Hall, London 7. Matheson, R. (2003). Medical entomology. Comstock publishing associates, N.Y 8. Mullen.G, Durden.L.Medical and Veterinary Entomology. 2002. Academic Press 9. Goddard.J. Infectious Diseases and Arthropods .2008.Humana Press ZOOL-503 Parasitology 3-1 1. Introduction to parasitology. Relationship to other sciences, parasitology and human welfare. Parasites of domestic and wild animals. Camers in parasitology. Some basic definitions 2. Basic principles and concepts. Parasite ecology and evolution 3. Basic principles and concepts. Immunology and pathology. Susceptibility and resistance, innate defence mechanisms. Acquired immune response in vertebrates. Immunity in invertebrates. Immunodiagnosis, pathogenesis of parasitic infections. Accommodation and tolerance in the host-parasite relationship 4. Parasitic protozoa, form, function and classification 5. Kinetoplasta, trypanosomes and their kin, forms of trypanosomatidae 6. Other flagellated protozoa, order Retortamonadita, order Diplomonadida, order Trichomonadida, order Opalinida 7. The Amoebas. Order Amoebida, order Schizopyrenida 8. Phylum Apicomplexa, Gregarines, Coccidia and related organisms. The apical complex, class Gregarinea, class Coccidea 9. Phylum Apicomplexa, Malana, organisms, and pyroplasms, order Haemospondea, order Pyroplasmida 10. Phylum ciliophora, ciliated protistan parasites, class Spirotoichea, class Litostomitea, class Oligohymenophorea 11. Phyla Microspora and Myxozoa. Parasites with polar filaments. Phylum Microspora, Phylum Myxozoa 12. The Mesozoa, pioneers or Degenerates. Class Rhombozoa, class orthonectida, Phylogenetic position, physiology and Host parasite relationship. Classification of Phylum Mesozoa Practicals Preparation of temporary and permanent slides and identification of parasitic protozoan and local helminthes of medical and veterinary importance. Section cutting of the infected tissues and the study of their pathology Books Recommended 1. Foundation of parasitology, 2000, 6th ed. Roberts, L.S. and Janovy, J. McGraw Hill Book Co 2. Protozoology, 1996, 2nd ed. Hausman, K. and hulsmann, N. Thieme medical publishers, Inc. New York 3. Noble and Noble, 1982. Parasitology. The Biology of animal parasites. 5thedition. Lea and Febiger 4. Beck, J.W. and Davies, J.E., 1981. Medical parasitology. 3rd edition. The C.V. Mosby Company, Toronto, London 5. Cheesbrough, M., 1987. Medical Laboratory Manual for Tropical Medicine. Vol.I. University Press Cambridge 6. Smyth, J.D., 1994. Introduction to Animal Parasitology. Cambridge University Press. 7. Roberts, L.S. and Janovy, J. Jr., 2005. Foundations of Parasitology. 7th Edition. W.M. Brown Publishers, Chicago, London, Tokyo, Toronto 8. Roberts, L.S. and Janovy, J. Jr. Foundations of parasitology. 7th Edition. 2005. Wm Brown Publishers, USA 9. Urquhart, G.M., Hucan, J.L., Dunn, A.M. and Jennings, F.W. Veterinary parasitology. 2000. Longman Scientific and Technical publications, Longman Group, UK *********************** SEMESTER-2 Course No ZOOL-504 ZOOL-505 ZOOL-506 ZOOL-504 Title Integrated Pests Management Application of Biotechnology Conservation and Wildlife Management/ Toxicology Preparation and Submission of Synopsis Credit Hrs 3-1 3-1 3-1 Integrated Pests Management 3-1 0-0 12 1. Fundamentals of pest management, Concepts and characteristics of pest management, Kinds of pests management strategies, Development of pest management programme Steps in establishing biological control 2. programmeme. Concept and significance of natural control. Attributes of an effective natural enemy. Super parasitism and multiple parasitism. Importance of biological control. Methods of colonization, recovery and evaluation of natural enemies. 3. Importance of chemical control: Classification of insecticides, stomach poison, contact poisons, fumigants. Integrated pest management Practicals 1. Collection and identification of insect pests of agricultural crops and forest trees. Demonstration of effects of some insecticides on the survival of insect pests in the Laboratory Books Recommended 1. De Bach, P., (1964). Biological Control of Insect Pests and Weeds, Chapman and Hall, London 2. Pedigo, L.P., 1991. Entomology and pest management. Maxwell MacMillan 3. Metcalf, C.L. and Flint. Destructive and Useful Insects, McGraw Hill Co., N Y 4. Martin, H. Insecticide and Fungicide, Handbook for Crop Protection, Blackwell, Oxford 5. Peshin.R.Dhawan. A. K. Integrated Pest Management: Volume 1: InnovationDevelopment Process.2009. Springer 6. Perveen, F. 2009a. Effects of chlorfluazuron on the reproduction of Spodoptera litura. Edited by A.G. Müller. 1st edition. VDM-Verlag Publisher, Berlin, Germany. p. 175. 7. Perveen, F. 2009b. Chlorfluazuron and embryonic development of Spodoptera litura. Edited by A.G. Müller. 1st edition. VDM-Verlag Publisher, Berlin, Germany. p. 105 8. Perveen, F. 2009d. Biochemical analyses of action of chlorfluazuron in Spodoptera litura. Edited by A.G. Müller. 1st edition. VDM-Verlag Publisher, Berlin, Germany. 9.Farzana Perveen (Ed.) (2012) INSECTICIDES-ADVANCES IN INTEGRATED PEST MANAGEMENT; ISBN 978-953-307-780-2; InTech Book Publisher, Rijeka, Croatia; 1-722; Online: http://www.intechopen.com/books/show/title/insecticides-advances-inintegrated-pest-management 10. Farzana Perveen and Anzela Khan (2013) Insecticidal Activity of Some Medicinal Plants. 1-117; ISSBN: 978-3-659-34834-1; Lambert Academic Publisher (LAP), Germany; Online: https://www.lappublishing.com/catalog/details//store/gb/book/978-3-659-28448-9/red-earedslider-turtle,-trachemys-scripta:-model-for-the-research ZOOL-505 Application of Biotechnology 3-1 Concepts, historical background, conventional biotechnology-green revolution Microbial Biotechnology Microbes as a tool in biotechnology bacterial growth, bacterial defense, restriction enzyme, Agrobacterium tumefaction (crown gall disease), nitrogen fixation (use in biotechnology introduction to important enzymes production, fermentation and its economic importance microorganism and biotechnological products, viruses role in biotechnology, conventional biotechnology, industrial biotechnology Plant Biotechnology Tissue culture, micropropagation. Biotech’s inputs in Medicinal plant research, Animal and Health Biotechnology Engineering for vaccines, genetic engineering for better animal production, introduction to AIDS vaccine and drugs and genetic engineering a hope for remedy, cloning, and artificial reproduction. Genetic engineering isolation cutting, joining and amplification (PCR) of DNA different methods of genetic transformation. Biohazards, bioethics (current status if bioethics in Pakistan), intellectual property rights Practicals 1. Cultivation of microorganisms and their nutritional requirements 2. Effect of antibiotics and chemical compounds on bacterial physiology 3. Isolation of microorganisms from food 4. Plasmid transformation in E. coli Books Recommended 1. White, D. (1999). The Physiology and biochemistry of Prokaryotes, 2nd Edition Oxford University Press 2. Jay, J. M. (1990). Modern Food Microbiology. D. Van Nostrand Company 3. Old R.W. and S.B. Primrose. (1994). Principles of Gene Manipulation, an Introduction to Genetic Engineering (4th edition). Blackwell Scientific Publications 4. Setlow J. K. (2000). Genetic Engineering: Principles and Methods. Kluwer Academic Publishers 5. Krishna.V.S. Bioethics and Biosafety in Biotechnology.2007. New Age International 6. Furr AK. CRC handbook of laboratory safety 5th edition. 2008. Boca Raton, FL, CRC press ZOOL-506 Conservation and Wildlife Management 3-1 Wildlife of Pakistan, identification, distribution, status, conservation and management (population estimate technology) of fishes, reptiles, birds and mammals of major importance in Pakistan. Philosophy and significance of wildlife conservation. Biodiversity and sustainability of wildlife. Wildlife rules and regulations in Pakistan. National and International agencies involved in conservation and management of wildlife. Sanctuaries, Game Reserves and National Parks in Pakistan. Ramsar convention, wetlands, endangered species of Pakistan Practical 1. Study of fauna of various Zoogeographical Regions of the World and Pakistan. 2. Map work for identification of various Zoogeographical Regions of the World and Pakistan Books Recommended 1. Bailey, J.A., 1986. Principles of Wildlife Management, John Wiley 2. Ali S. and Ripley S.D., 1973. A Handbook of Birds of India and Pakistan, Oxford University Press, London 3. Roberts, T. J., 1998. The Birds of Pakistan, Vol. II. Oxford 4. Roberts, T. J., 1992. The Birds of Pakistan, (Vol. II), Oxford 5. Roberts, T.J., 1977. Mammals of Pakistan. Ernest Benon Ltd, London, 6. Robinson, W.L. and Bolen, E.G., 1984. Wildlife Ecology and Management. McMillan, Cambridge 7. Magon, C.F. 1988. Biology of freshwater pollution. Longman and Scientific 8. Boyd, C.E and Tucker, (1998) Pond aquaculture andWater quality management. B oston, Kluwer publishers alabama 9. Ali, S.S. (1999). Paleontology, Zoogeography and wild-life management 10. Woodroffe .R, Thirgood.S, Rabinowitz.A.People and Wildlife .2008. Cambridge University Press 11. Perveen, F. 2009. A beauty of Indus River: the Indus River dolphin, Platanista minor Owen, its threats and protection in N-WFP, Pakistan Edited by A.G. Müller. 1st edition. VDM-Verlag Publisher, Berlin, Germany. p. 102. Prepration and Submission of Synopsis 0-0 Students have to prepare their synopsis with consult to their supervisor and submit in this semester (2nd Semester) for approval by the Board of Studies. Semester-III Research 4 Those students who have submitted their synopsis, should start their research and continue to the end of this semester (3rd Semester). Semester-IV Research and Thesis 4 In this semester (4th Semester), students have to complete their research, write and defende their thesis by presentation and viva voce. Seminar 2 Befor defending the thesis, students have to present their research in the seminar infront of students and faculty members of the department. **************