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Download Discussion Section Handout March12 MCB141 103/104 How did we
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Discussion Section Handout MCB141 103/104 March12 How did we learn that mesodermal fate is induced, instead of achieving mesodermal fate autonomously? What does a Nieuwkoop recombinant become if it is made up of an animal cap and ventral vegetal tissue? What does a Nieuwkoop recombinant become if it is made up of an animal cap and dorsal vegetal tissue? How did we determine what the mesoderm inducer was? What would you get from a Nieuwkoop recombinant whose vegetal tissue had been injected with Nodal morpholino? What would you get from a Nieuwkoop recombinant whose animal cap had been injected with mRNA (non-limiting amounts) for a dominant negative form of the Nodal receptor subunit I? What strategies does the embryo employ to increase Nodal signaling at the Nieuwkoop center? What pathways execute these strategies? Why doesn’t mesoderm form in cells expressing VegT? Why do embryos totally depleted of VegT form only ectoderm? Why is injection of B-catenin into the Animal-most cells insufficient to induce an organizer there? Why turn on Wnt inhibitors downstream of Wnt signaling? What would happen if you injected large quantities of mRNA for a BMP receptor subunit I whose kinase domain recognizes Smad2/3 instead of Smad1/5/8? For practice, draw the Nodal and BMP signaling pathways? How are these related? What key molecule do they both require?
