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School of Biomedical, Biomolecular and Chemical Sciences Unit Outline Molecular Biology of the Cell SCIE1106 Semester: 2 Campus: Crawley Unit Coordinator: Dr Martha Ludwig This outline is the currently available version for this unit. Detailed Information on unit content and assessment may undergo modification before the time of delivery of the unit. For the most up to date information students must consult material supplied to enrolled students by the unit co-ordinator. All material reproduced herein has been copied in accordance with and pursuant to a statutory licence administered by Copyright Agency Limited (CAL), granted to the University of Western Australia pursuant to Part VB of the Copyright Act 1968 (Cth). Copying of this material by students, except for fair dealing purposes under the Copyright Act, is prohibited. For the purposes of this fair dealing exception, students should be aware that the rule allowing copying, for fair dealing purposes, of 10% of the work, or one chapter/article, applies to the original work from which the excerpt in this course material was taken, and not to the course material itself © The University of Western Australia 2001 1 Introduction This is an introductory unit that explores prokaryote and eukaryote cell structure and function at the physiological and molecular level. A strong emphasis will be placed on applications in biomedicine and biotechnology. The first part of the unit will cover DNA and RNA structure and replication, gene expression and its regulation, and recombinant DNA technology. The second part of the course will deal with the cell cycle and cell differentiation, cell structure and compartmentalization, the structure of biological membranes and strategies used to move molecules across these membranes, and intercellular communication. Applications of cell and molecular biology in microbiology, disease diagnosis and therapy, and genetic engineering will be discussed in the final part of the course. Practical classes will illustrate fundamental techniques in cell and molecular biology, and tutorials will discuss recent advances in microbiology, physiology, and biochemistry. Advisable Prior Study Core Concepts in Biology (BIOL1130) or Human Biology (ANHB1101) with normally 12 points of Level 1 chemistry as a co-requisite. Technical Requirements Laboratory Notebook: You must record everything during laboratory classes in a dedicated Notebook. The notebook must have a hard cover, with pages that are properly bound (a Science Exercise Book with graph paper/ruled pages interleaved is very suitable). A laboratory coat and closed-toe shoes must be worn during the practical sessions. Calculator: You should have a pocket calculator available for use in the laboratory; it should have the means for determining the log of a number. Software Requirements Students are required to arrange their own access to word processing and spreadsheet and graphing software. Microsoft Word and Excel are recommended. Microsoft Powerpoint and/or Adobe Reader are required for viewing lecture material on WebCT. Contact Details Unit web site (may include Web CT URL): http://Webct.uwa.edu.au/ Name of Unit coordinator: Dr Martha Ludwig e-mail: [email protected] Phone: 6488 3744 Fax: 6488 1148 Location: Chemical and Molecular Sciences Building, room 3.04 Consultation hours: By appointment 2 Unit Structure Summary Lectures Three lectures per week. Practical and/or Laboratory Sessions One 2 hr laboratory class every two weeks. Attendance is compulsory. Tutorials One 2-hour tutorial every second week, alternating with the practicals. Attendance is compulsory. In each tutorial, the previous laboratory and lecture material are discussed. Topics For details of times and venues of all teaching sessions please consult the most recent version of the university timetable at http://www.timetable.uwa.edu.au/ LECTURE TOPICS 1 Overview of Unit 2 Macromolecules 3 Structure of prokaryotic and eukaryotic cells 4 Structure of DNA 5 Replication of DNA 6 Mutation and repair of DNA 7 Mechanisms of transcription 8 Products of transcription 9 Translation of mRNA into proteins 10 The process of translation 11 REVISION LECTURE 12 Introduction to protein structure 13 Protein function 14 Introduction to cloning 15 Copying DNA and RNA in the test tube 16 Hybridisation techniques 17 REVISION SESSION 18 Regulation of gene expression in prokaryotes 19 Negative regulation of the E. coli lactose operon 20 Positive regulation of the E. coli lactose operon 21 Regulation of gene expression in eukaryotes 22 Post-transcriptional regulation of eukaryotic genes 23 REVISION LECTURE 24 Cell cycle and programmed cell death 25 Cytoskeleton, cell shape and motility 26 Motility and cell division 3 LECTURE TOPICS continued 27 Secretory pathways and endo/exocytosis 28 Membrane structure/lipids/fluidity 29 Membrane transport 30 Molecular and cellular symbiosis: mitochondria and chloroplasts 31 Polarised cells – design and function 32 Intracellular and intercellular communication and signalling 33 Sensitive and self-sensitive membranes 34 REVISION LECTURE 35 Molecular diseases 36 Genetic engineering of plants 37 Microbial biotechnology 38 Ethical issues in molecular biology 39 REVISION LECTURE References, Resources and Reading Materials Essential Cell Biology (2nd Edition) Alberts et al., Garland Science Press ISBN 0-8153-3481-8. If you wish to continue in Molecular Biology in years 2 and 3 get Molecular Biology of the Cell (4th Edition), Alberts et al., Garland Science Press ISBN0-8153-4072-9. (ISBN numbers are for the softcover editions.) The unit manual, supplied to all students who enrol in the unit, contains detailed laboratory notes, assignment instructions, and other supporting material. Unit Web Site All enrolled students have access to the unit WebCT site (http://webct.uwa.edu.au). This site will be used for notices, posting of supplementary course materials, email and discussion. It is strongly recommended that you log onto this site on a daily basis. The website should be used for all remote communication to the unit co-ordinator. Assessment Details Assessed Work % Mark Timing Lecture quizzes (6, in tutorials) 20% Throughout semester Lab quizzes (6, in tutorials) 20% Throughout semester Final lab report and laboratory notebook 10% Last tutorial of semester Final exam (3hr exam, multiple choice questions) 50% End of semester exam period 4 Plagiarism The University of Western Australia takes very seriously the matter of academic misconduct by students and has policies in place that define misconduct (including plagiarism) and the penalties that apply. The consequences for misconduct can be severe, including exclusion from the university. All students are expected to make themselves aware of the definitions and policies relating to academic misconduct, found at the websites below, and with any additional requirements or stipulations that may be provided by individual unit co-ordinators. http://www.secretariat.uwa.edu.au/__data/page/20839/StuMiscondInfo.rtf http://www.teachingandlearning.uwa.edu.au/tl/academic_conduct 5