Download No host specificity concerning in vitro adhesion of probiotic lactic

PROBIOTIC LAB CAUSED PERSISTENT CHANGES IN THE CANINE JEJUNAL
MICROBIOTA
Rinkinen, M. L.1, Manninen. T.2, Beasley, S.2 and Saris, P. E.2
1
Department of Clinical Veterinary Sciences, University of Helsinki, Helsinki, Finland
2
Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki,
Finland
The aim of the study was to investigate the stability of potential probiotic lactic acid
bacteria (LAB) in the canine small intestine. Most of the earlier studies have examined
the competitive exclusion of pathogenic bacteria, whereas reports of the potential effects
on the endogenous LAB population are scarce.
Five lactic acid bacteria strains isolated from dog faeces (Lactobacillus fermentum, L.
salivaruius, L. rhamnosus, L. mucosae and Weissella confusa) were evaluated in this
study. The strains were fed to five permanently fistulated dogs for 7 days and the stability
of the LAB strains were monitored for 17 days by plating jejunal content on mLBS. The
chromosomal DNA of the bacterial colonies was cloned by PCR and run in DGGE to
detect the living bacteria passing through the intestine. The effect of the supplemented
LAB on the normal intestinal LAB microbiota was determined by DGGE.
All the fed LAB strains survived the passage through the upper GI tract but disappeared
within 7 days after the supplementation had ceased. However, a significant and persistent
change in the indigenous LAB microbiota caused by the fed LAB lasted more than 17
days after the cessation of LAB supplementation.
We conclude that this long-term modification of the indigenous intestinal LAB
microbiota may be an important mechanism for probiotic LAB to exert their beneficial
health effects.