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Location of Retropharyngeal Lymph Nodes in Sheep The medial retropharyngeal lymph nodes are located just above the pharynx and larynx, and medial to the hyoid bones. The nodes are not immediately apparent when the head is removed through the atlanto-occipital joint as they are covered by the hypaxial muscles attaching to the base of the brain, as well as by fat and salivary tissue (Figure 1), but they can be easily palpated by running a finger along the inside of each hyoid bone. The nodes are discrete, oval and not lobulated like the adjacent salivary glands. Their location in relation to other landmarks, after removal of fat and overlying muscle, is illustrated in Figure 2. Figure 1: Head removed through the atlanto-occipital joint. The hyoid bones and medial retropharyngeal nodes are concealed by hypaxial muscles (M), fat and salivary tissue. Note the carotid arteries (C), parotid lymph node (PLN) and parotid salivary glands (PSG). PSG PSG M PLN C HB RLN Figure 2: After removal of the overlying muscular tissue and fat, the medial retropharyngeal lymph nodes (RLN) are clearly visible, dorsal to the pharynx and medial to the hyoid bones (HB).