Download Syllabus for BIOL 277 Immunology - 0219

Syllabus for BIOL 277 Immunology - 0219 - S15
1 of 4
https://shoreline.instructure.com/courses/1119655/assignments/syllabus
Jump to Today
Course Syllabus
Biol 277 Immunology – Spring 2015
Item 0219
Instructors:
Guy Hamilton ([email protected] (mailto:[email protected]) ) 206-546-4786
Dina Kovarik ([email protected] (mailto:[email protected]) ) 206-679-4017
Office hours:
Guy: Mon, Tues, Thr 11:00pm –1:00pm (room 2809 or the Biology Learning Center)
Dina: Tues 2:00-3:00 PM in Biotech Lab (Room 2930), Wed 4:00-5:00 online & by appointment
Textbooks:
There is no required textbook for the lecture or lab portions of the course. However, students are encouraged to read the chapters listed below from Kuby
Immunology, 6th Edition.
There are copies of this textbook in the Biotech lab and in the library for short-term loans.
Dina purchased a used copy on Amazon for $25.89 (and was happy to have an updated edition from the Kuby Immunology 3rd Edition she bought for her
immunology course as an undergrad)
The full text of the 6th Edition is available for free from Google Books (https://books.google.com/books?id=oOsFf2WfE5wC&printsec=frontcover&
dq=kuby+immunology+6th+edition&hl=en&sa=X&ei=aD8TVbmdNNfooASy9oDIDg&ved=0CB0Q6AEwAA#v=onepage&q=kuby%20immunology%206th%20edition&
f=false)
A nice companion for the lab portion of immunology is Immunochemical Techniques Laboratory Manual
(http://smile.amazon.com/Immunochemical-Techniques-
Laboratory-Manual-Goers/dp/0122870484/ref=sr_1_1?s=books&ie=UTF8&qid=1427325005&sr=1-1&keywords=Immunochemical+Techniques+Laboratory+Manual) (1993). It
was written by a biochemistry professor for immunology instructors and students, and provides very useful background on immunological techniques. We will be
using some of the protocols from this manual (which Dina will copy and post as PDFs to the Modules section of Canvas). There are also 2 copies in the Biotech Lab
for short-term loan. Dina purchased a used copy from Amazon for $11.92 plus $3.99 shipping. The remaining laboratory protocols will be posted to Canvas at least
one week prior to each week's lab experiments.
Lecture Topics, Lab Topics* and Exam Dates
* Note: Lab Topics are provided below as a general overview. Specific experimental details and daily activities will depend upon the progress made in each lab
session. Click here for the most current (03/26/2015) version of the Overview of Experiments (https://shoreline.instructure.com/courses/1119655/files/50669884
/download?wrap=1) Preview the document (https://shoreline.instructure.com/courses/1119655/files/50669884/download?wrap=1) View in a new window
(https://shoreline.instructure.com/courses/1119655/files/50669884/download?wrap=1) .
Week of
Topics
Reading
March 30th
Lecture:
- Overview of components of the
Immune System
Chapters 1, 2
3.2 and 3.4, 13.1
- Antibody Structure and function
- Hematopoesis and Organs of the
Immune system
Lab:
Blood Smears, Staining and
Differentials
April 6th
Lecture:
- Organization and Expression of Ig
Chapter 7
genes, B-cell receptors
Lab:
IgG Purification from Serum & gDNA
Purification from Blood
April 13th
Lecture:
- Generation of T-cell receptor
diversity
Chapter 7
4/17/2015 6:01 PM
Syllabus for BIOL 277 Immunology - 0219 - S15
2 of 4
https://shoreline.instructure.com/courses/1119655/assignments/syllabus
April 17th EXAM 1 Available online
and due Sunday, April 19 by
midnight
Lab:
SDS-PAGE Gels of Purified IgG
April 20st
Lecture:
-MHC receptors and Antigen
Presentation
Chapters 8
Lab:
AP Western blots of Purified IgG
April 27th
Lecture:
-T-cell activation and maturation
Lab:
Odyssey Western blots of Purified
IgG
Chapter 9, 11
May 4th
Lecture:
- B-cell maturation and activation
May 5th: Project Article Choice
(though article may be submitted to
Dina for review prior to this date)
May 8th EXAM 2 available online
and due Sunday, May 10th by
midnight
Chapter 10, 12
Xconomy Forum Wed, May 6
Lab:
Lab Only on Tuesday [see Overview
of Lab Experiments]
May 11th
Lecture:
- Continue B-cell maturation and
activation
- Cytokines
Chapter 10, 12
Chapter 5
May 12th: Project Paper Due
(though papers may be submitted to
Dina for review prior to this date)
Lab:
ELISAs with Purified IgG. Planning
Student Projects. Submit Plan to
Dina for Review.
May 18th
Lecture:
Infectious Diseases, Vaccines and
the Immune system
Lab: Student Projects
May 19th: Draft of Experimental
Design due to Dina
Chapter 19, 20
May 25th
Lecture:
Chapter 21
Cancer and the Immune system
May 29th EXAM 3 available online
and due Sunday, May 31st by
midnight
Lab: Student Projects
June 1st
Lecture:
Student Presentations
June 2rd & 4th
Lab:
Tues, June 2: Student Projects
Thurs, June 4: Student Project
Presentations
FINALS
WEEK
June 11th
Lecture:
Thursday June 11th, 3:30-5:20 PM
FINAL project due (take
4/17/2015 6:01 PM
Syllabus for BIOL 277 Immunology - 0219 - S15
3 of 4
3:30-5:20
PM
https://shoreline.instructure.com/courses/1119655/assignments/syllabus
home)
In-Class Student
Presentations
Lab:
Final Lab Report Due (no lab final
during final exam time, Thursday,
June 11, 5:30-7:20 PM)
Course Structure
This is a hybrid course. The lecture material for each week will be posted on Mondays. The lectures will be posted under the “Modules” link. The class meetings will
consist of lecture discussion or exams or student presentations. The lecture discussion will be based on the lecture questions Guy or Dina post with the lectures.
The last 2 weeks of the course (June 2-4 and June 11) will be student presentations, described in the next section.
Individual Project and Presentations: Immune Disease and Emerging Therapies
Each student will choose an immune disease or disorder and search the literature for the current treatment protocols as well as the experimental protocols. It is then
the goal to identify a basic research journal article that has provided the rationale for the development of this new treatment. Take this hypothetical example. Consider
the standard treatment of certain types of leukemia is a bone marrow transplant; however, a new treatment uses a drug that is a specific protein kinase inhibitor. A
student could find the research that identified the gene (encoding a protein kinase) that plays an important role in the development of the disease or the research that
demonstrated that the inhibitor was specific to the kinase. These results could be summarized and then related to the potential success of the clinical use of the drug.
In this way, each student will identify how basic research has led to new treatments for immunological disorders. The project has two parts.
A written 4-5 page report summarizing your research on the disease and the research article you will present in the class.
The oral presentation will be done in class and will 10-12 minutes in length, plus 2-3 minutes for questions & answers. Dina will give each student feedback on
the written report to help improve the organization of the oral presentation. The grade received will be based on both the oral and written components of the
project.
Project Due Dates:
Article choice – May 5th
Written Report – May 12th
Oral Presentations – June 2nd, 4th, 11th
What is required of you?
1) Attendance The level of material presented in the lecture course is fairly advanced and the best way to ensure your success is to attend lectures. The textbook for this class is
best used as a reference to help you understand topics presented in the class. We do not lecture straight from the book, so it will be difficult to grasp everything we
present in the lecture simply by reading the textbook.
It is even more important to be able to attend labs. The lab experience is a critical portion of this course that requires that you perform the experiments with your own
hands. Also, you will be working closely with a lab partner and it would be unfair to make your lab partner perform experiments for you. Finally, some of the labs run
for multiple days, so missing one day can result in your inability to complete multiple labs. If you cannot attend lab on a particular day or week, it will require you to
schedule a time to come to lab outside of the class meeting to make up the lab within a reasonable amount of time. It is your responsibility to coordinate with your lab
partner and the instructor (Dina Kovarik) or Jim Schultz to find an acceptable time.
2) Grading – subject to change at the instructors' discretion
Because of the interconnected nature of the lab and the lecture course, your final grade will be based on your performance in both classes. It will break down as
follows:
3 Lecture exams + Article Presentation + Final Project: 50%
Laboratory Participation, Notebook, Data Presentations and Final Project: 50%
(To be explained by Dr. Dina Kovarik)
There will be 3 lecture-based exams that will focus on the material covered up to that point; however, because all topics in the course build on one another, every
exam will essentially be cumulative. In other words, if you do poorly on the first exam, it benefits you to go back and learn that material, because those topics will be
the building blocks for topics introduced in the remainder of the course.
4/17/2015 6:01 PM
Syllabus for BIOL 277 Immunology - 0219 - S15
4 of 4
https://shoreline.instructure.com/courses/1119655/assignments/syllabus
Laboratory notebooks must be maintained by every student and will be graded at multiple times during the quarter. They will be judged on how well the background
and purpose of the experiment is explained, experimental data is presented, results are interpreted, and questions (assigned by the instructor) are answered. More
information regarding lab notebook requirements can be found in a separate file in the course documents folder.
3) Plagiarism and Cheating –
To be precise, the definition and consequences of cheating and plagiarism are in compliance with the applicable Washington Administrative Codes (WACs) and
campus policy (compliant with Policy #5033, Dishonesty in Academics). Any student caught cheating on an exam or quiz will receive no credit for that assignment.
Laboratory work will be done in groups and while it is required that you work with your lab partner to complete labs, each student's laboratory notebook must be their
own work. Blatant copying of laboratory discussions and conclusions in the notebook will result in no credit for that assignment. Note that in these cases, both
students will receive no credit for the assignment. For the student presentations, plagiarism of materials from the web or from scientific journals without proper
citation will result in no credit for the assignment.
What About the Lab?
A detailed lab schedule will be posted HERE, though this schedule will be subject to change based on the progress made on experiments during each lab session.
The overall goals of the lab portion of this course are to familiarize students with common immunological techniques used in various biotechnology-related fields and
to model what is typically expected from scientists in research settings. To this end, your grade for the laboratory portion of this class will be based on the following:
Completing your work / experiments on time [essentially daily participation]: 25%
Creating an accurate, thorough record of your work [your lab notebook]: 25%
Communication about your work [through regular presentations in lab]: 25%
Using what you have learned in lab, in collaboration with your lab partner, to design, implement and optimize an experiment: 25%
Design an ELISA (details of available reagents will be provided in class and Dina will work with lab groups during this process, including reviewing your
experimental plan by May 7, 2015)
Implement your experimental design
Optimize your experimental design (i.e., repeat the experiment with intentional modifications)
Report your findings in writing (i.e., a traditional 5-7 page Lab Report) and verbally (i.e., a 8-10 minute presentation of the rationale, design, implementation,
optimization and conclusions of your experiments)
Date
Details
Fri Apr 4, 2014
Week 1 participation (https://shoreline.instructure.com/courses/1119655/assignments/5149498)
due by 11:59pm
Sun Apr 19, 2015
Exam #1 Biol 277 Sp15 (https://shoreline.instructure.com/courses/1119655/assignments/5149497)
due by 11:59pm
Sun May 10, 2015
Exam #2 Biol 277 Sp15 (https://shoreline.instructure.com/courses/1119655/assignments/5149494)
due by 11:59pm
Sun May 31, 2015
Exam #3 Biol 277 Sp15 (https://shoreline.instructure.com/courses/1119655/assignments/5149496)
due by 11:59pm
Thu Jun 11, 2015
Final Exam/Project (https://shoreline.instructure.com/courses/1119655/assignments/5149500)
due by 5pm
Final Lab grade % (https://shoreline.instructure.com/courses/1119655/assignments/5149502)
Presentation (https://shoreline.instructure.com/courses/1119655/assignments/5149501)
Week 2 participation (https://shoreline.instructure.com/courses/1119655/assignments/5149499)
4/17/2015 6:01 PM