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List two manufacturing processes that depend on the activity of yeast. List two growing conditions commercial brewers must control for yeast activity. (credit) ANS raising of bread dough and making wine and beer ANS optimum temperature + optimum pH What type of organism is yeast? What is meant by the term batch process? (credit) ANS A single celled fungus ANS All necessary ingredients are added and then the process is closed until product produced. What is the food source for yeast? Why are the barley grains malted before using them in the brewing industry? (credit) ANS Sugar What is the other name for anaerobic respiration by yeast? Why does milk go sour? (credit) ANS Fermentation What is the equation for anaerobic respiration in yeast? ANS sugar ANS To produce maltose sugar for the yeast cells. energy + CO2 + Alcohol List 3 differences between fermentation an aerobic respiration in yeast. (credit) ANS fermentation provides less energy and alcohol ANS Bacteria in the milk use the lactose sugar to carry out fermentation and produce lactic acid. Describe one way the environment can be damaged by the disposal of untreated sewage. Why are bacteria important in the carbon cycle? (credit) ANS Pollute our river and lochs and kill the fish ANS The bacteria release CO2 from dead organisms and waste as they carry out respiration List one disease that can be spread by untreated sewage. What do bacteria gain from carrying out decomposition? (credit) ANS typhoid / cholera ANS The bacteria use the waste or dead organisms as a food supply to obtain energy. List two precautions that will be taken when working with micro-organisms. Why is complete breakdown of sewage only possible in aerobic conditions? (credit) ANS Sterilise the working area and equipment, work close to a bunsen burner, wear a lab coat and tie back your hair if long ANS As the sewage is completely broken down to CO2 and H2O What precautions must be taken in the manufacturing process to deal with resistant bacterial and fungal spores. (credit) Why is a range of micro-organisms required to break down the materials in sewage? (credit) ANS Use high pressure or strong chemicals List the process in the nitrogen cycle that uses bacteria. (credit) ANS decomposition, nitrification, nitrogen fixation and denitrification List two ways oxygen can be supplied during sewage treatment. ANS By mechanical agitation or pressure ANS To breakdown all the different types of material found in sewage What is the main process in the treatment of sewage? ANS The breakdown of sewage into harmless products by the decay microorganisms. What useful product can manure be changed in to? (credit) What is the round chromosome called in a bacterial cell? (credit) ANS High energy methane gas ANS Plasmid List two products of fermentation. What is genetically engineered bacteria? ANS alcohol and methane gas ANS bacteria that contain pieces of chromosomes from a different organism What can the waste product whey be changed into? (credit) ANS high protein cattle feed List one advantage of using fuels form fermentation compared to using fossil fuels? ANS fuels from fermentation are renewable (unlimited supply) What form of reproduction do microorganisms use? ANS asexual reproduction Micro-organism be grown to produce what type of useful substances. ANS micro-organisms can be used as a protein rich food Why do we genetically engineer chromosomes? ANS So the bacteria make a new substance that is useful to humans. When the plasmid chromosome is removed from the bacteria what has to be added to it? (credit) ANS A useful gene like the insulin gene Why is it better to produce insulin using genetically engineered bacteria? (credit) ANS It can be produced quickly and in large quantities List two advantages of creating a desired organism by genetically engineering compared to selective breeding? (credit) ANS It is quicker and greater chance of obtaining desired organism Name one useful substance produced by genetic engineering. What is the function of antibiotics? ANS Insulin ANS To prevent the growth of micro-organisms Why is there an increasing demand for insulin produced by genetic engineering? (credit) Why do we need a range of antibiotics when treating bacterial diseases? (credit) ANS There are more people with diabetes. ANS As some bacteria have become resistant to certain antibiotics What do biological detergents contain? List two advantages of using immobilization techniques in continuous flow techniques. (credit) ANS enzymes made by bacteria List two advantages of using biological detergents. (credit) ANS less energy used to heat the water and it is less harmful to delicate fabrics What enzyme group do the protein-digesting enzymes belong to? (credit) ANS The protease group What is the function of the enzymes found in the biological washing powders? (credit) ANS The enzymes digest the food stain on the clothes ANS The jelly beads can be used over again and also separated easily from the product. What is meant by immobilization techniques? (credit) ANS By adding either enzymes or yeast to a jelly bead List one advantage in using continuous flow processing compared to using the batch process. (credit) ANS continuous flow uses immobilization