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Engineering E.Coli to see Light
Voigt CA et. al. 2005
Light Engineering Imaging using E.Coli
System
Stimulus
Sensor
Signal
Transducer
Effector
Output
Stimulus



Red Light
Highly defined edges
Two-dimensional image
Sensor

Phytochrome



Membrane bound extracellular sensor
Intracellular regulator
Fusion protein: phytochrome and
EnvZ histidine kinase domain
Extracellular
sensor
Intracellular
regulator
Photoreceptor Expression



PCB not produced in E.Coli
Introduction of PCB synthesis genes
Converts haem into PCB
Transduction Mechanism

Red light represses EnvZ autophosphorylation
Output


OmpR-P activates promoter linked
to reporter gene
lacZ reporter produce black
compound
OmpR-P dependent
ompC promoter
System Revisited
Review


Created novel genetic circuit
High definition film



(~100 megapixels per square inch)
lacZ expression as a function of
light intensity / wavelength
Transfer function
Something for the team
to think about!!
Light Canon
Characterisation

Macro


Resolution
Micro


Transfer function
SigB activity as a function of:
Intensity
 Wavelength
