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Placental Growth Hormone-20K (human) PRODUCT INFORMATION Description Recombinant Placental Human GH-20K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20,498 Dalton. Predicted pI = 8.20. rHupGH-20K is devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs. Placental growth hormone, or growth hormone variant (GH-V), is a member of the growth hormone gene family. Its physiologic role during pregnancy remains undefined. Although extensive work has been done characterizing the signaling pathways activated by hGH, the signaling pathways which are activated by GH-V have not been characterized. Human placental GH (hPGH) replaces pituitary GH during pregnancy. hPGH is correlated to serum IGF-I in normal pregnancies and in pregnancies complicated by fetoplacental disorders. rHupGH-20K is purified by proprietary chromatographic techniques. Cat. No.: Z00345 Size: 200 ug or 1 mg or 100 ug Source: Escherichia Coli. MW: 20,498 Dalton Endotoxin Level: Less than 0.1 ng/μg (IEU/μg) of rHupGH-20K. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Version: 10/26/2006 Purity Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl+50 nM NaCl, pH 11. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.14 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of pGH-20K as a Reference Standard. Specific Activity Fully biologically active when compared to World Health Organization (WHO) reference standard. Storage Lyophilized rHupGH-20K although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution and filter sterilization rHuGH-20K-pl can be stored at 4 °C for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Formulation The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3 previously adjusted pH 11. Reconstitution It is recommended to reconstitute the lyophilized rHupGH-20K in 0.4% NaHCO3 or water adjusted to pH 11, not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Sequence analysis The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile. ** For non-clinical research use only. ** Tel: 732-885-9188 Fax: 732-210-0262 E-Mail: [email protected] www.genscript.com