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Columbia Stem Cell Day 2014 April 1, 2014 Myrna Daniels Auditorium and the Riverview Terrace; Vivian and Seymour Family Heart Center; 173 Fort Washington Avenue, New York, NY 10032 Photo: Míriam Navarro Sobrino Photo: Masa Yazawa Program Program inquiries: Hynek Wichterle [email protected] General inquiries: Dola Sengupta [email protected] Columbia Stem Cell Initiative Tapping the Potential of Stem Cells for Human Health 2 Columbia Stem Cell Initiative Tapping the Potential of Stem Cells for Human Health Columbia Stem Cell Day April 1, 2014 9:30 AM Breakfast 10:00 AM Welcome 10:05 AM Asa Abeliovich 10:30 AM John Dittmar (Rothstein lab) 10:45 AM Laura Johnston 11:10 AM Coffee break+ poster session 11:55 AM Stephen Tsang 3 12:20 PM LUNCH/Poster session 1:40 PM Overview of the Stem Cell Core Facility 1:55 PM Cathy Mendelsohn 2:20 PM Larry Luchsinger (Snoeck lab) 2:35 PM Coffee break+ poster session 3:25 PM Joji Fujisaki 3:50 PM Lee Goldman 4:00 PM keynote lecture Peggy Goodell Columbia Stem Cell Initiative Tapping the Potential of Stem Cells for Human Health Short talks CLIK: A method using gene interaction density to assess genome-wide screen results reveals the importance of ribosome function in the decision to differentiate into a stem cell. John Dittmar, Robert J.D. Reid, and Rodney Rothstein Columbia University Medical Center, Dept. Genetics & Development, 701 West 168th Street, New York, NY 10032 4 The Role of Prdm16 and Mitochondrial Dynamics in Hematopoietic Stem Cell Function Larry Luchsinger, Kenjiro Kamezaki, David Corrigan, & Hans-Willem Snoeck Columbia Stem Cell Initiative Tapping the Potential of Stem Cells for Human Health Posters Heart Center Riverview Terrace Electrophysiology and Ca2+ Imaging in the Helmsley Stem Cell Core Facility at Columbia University Cholinergic Signaling Promotes the Activation of Adult Quiescent Neural Stem Cells Damian Williams, Kevin Sampson, Robert Kass, Amy MacDermott Alex Paul and Fiona Doetsch Columbia University Stem Cell InitiativeDepartments of Pathology and Cell Biology, Pharmacology, Physiology, and Neuroscience Helmsley Stem Cell Core Facility: Cell Production Facilities for Motor Neurons and Cardiomyocytes: Dosh Whye 1,2, Ray Funahashi 1,2, Dario Sirabella 1,2, Christopher E. Henderson 1,2, Hynek Wichterle 1,2, Gordana Vunjak-Novakovic and Barbara Corneo 1,2 High Efficient Reprogramming of Patient Skin Fibroblasts for Disease Modeling LouJin Song1,2,3, Elizabeth Han1,2,4 Daniel W. Awari1,2 and Masayuki Yazawa1,2,3 1Columbia University Stem Cell Initiative, CUMC; 2Department Rehabilitation and Regenerative Medicine, CUMC; 3Department Pharmacology, CUMC; 4Barnard College of of Department of Pathology and Cell Biology 2 Center for Motor Neuron Biology and disease Columbia Genome Center Screening Core Facility High Throughput Andrea Califano, Christopher E. Henderson, Charles Karan, Hai Li, Sergey Pampou, Ronald Realubit, Jan Michael Austria Generation of induced pluripotent stem cells from revertant mosaic keratinocytes N Umegaki-Arao 1, A M.G. Pasmooij 3, M Itoh 1, J E. Cerise 1, Z Guo 1, A Gostyński 3, M F. Jonkman 3, A M. Christiano 1, 2 Dept of Dermatology 1 and Genetics and Development 2, Columbia University, New York; Dept of Dermatology, University of Groningen 3 The Organic Chemistry Collaborative Center (OCCC) Black Building 1029, CUMC Campus Generation of induced pluripotent stem cells from conjunctiva Shi-Xian Deng 1,2,3, Donald Landry1,2,3, Alison Rinderspacher1,2,3 Jin Yang Yao Li Deniz Erol Wen-Hsuan Wu Yi-Ting Tsai XiaoRong Li Richard J. Davis Stephen H. Tsang 1Department of Medicine; 2Columbia University Medical Center; 3Columbia Stem Cell Initiative Statins Promote Axonal Regeneration by Inhibition of Protein Prenylation Hai Li1,5, Takaaki Kuwajima1, Elena Nikulina3, Jianwei Hou3, Wan Seok Yang1,2, Timothy Spencer1, Gist Croft1,2, Dosh W. Whye1,2, Emily R. Lowry1,2, Nuno J. Lamas1,2, Hynek Wichterle1,2, Marie T. Filbin3,*, Brent Stockwell1,4, Christopher E. Henderson1,2 Stem Cell Initiative, Center for Motor Neuron Biology and Disease, Departments of Rehabilitation and Regenerative Medicine, Pathology and Cell Biology, Neurology and Neuroscience2Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research3Biology Department, Hunter College, City University of New York4Howard Hughes Medical Institute, Department of Biological Sciences and Department of Chemistry, Columbia University5NYSTEM High-Throughput Screening and Chemistry Shared Facility Bernard and Shirlee Brown Glaucoma Laboratory, Edward S. Harkness Eye Institute, Columbia University Medical Center, 160 Fort Washington Ave Research Annex, Room 513, New York, NY 10032, USA Mitochondrial Dynamics in Human Embryonic Stem Cells Melanie Mumau, Larry Luchsinger, Hans Snoeck 1Columbia *this work is dedicated to the memory of Marie Filbin, deceased January 15th, 2014 5 Epigenetic Modifiers of iPSC Reprogramming Claudia A. Doege1, Keiichi Inoue1, Skylar Travis1, David B. Rhee1, Ryousuke Fujita1, Paolo Guarnieri2, Alan Shih3, Ross L. Levine3, Emily I. Chen4, Asa Abeliovich1 1 Columbia University, Depts. of Pathology, Cell Biology, Neurology, New York, NY 2 Columbia University, Biomedical Informatics Shared Resources, New York, NY 3 Human Oncology & Pathogenesis Program, MSKCC, New York, NY 4 Stony Brook University Proteomics Center, Stony Brook, NY Columbia Stem Cell Initiative Tapping the Potential of Stem Cells for Human Health The Role of PRDM16 in Leukemic Stem Cell Maintenance Human iPS Cell Derived Cardiac Microtissue Platform for Predictive Toxicity Studies David Corrigan Kacey Ronaldson, Stephen Ma, Timothy Chen, Keith Yeager, Dario Sirabella, and Gordana Vunjak-Novakovic Hans Snoeck Lab Laboratory for Stem Cells and Tissue Engineering Department of Biomedical Engineering, Columbia University Conditioning of stem cells by electrical, mechanical and immunological stimuli Aida Llucia-Valldeperas1, Donald O. Freytes2, Carolina SolerBotija1, Benjamin Sanchez3, Ramon Bragos4, Antoni Bayes‐ Genis1, Gordana Vunjak-Novakovic5. 1 ICREC Research Program, Health Science Research Institute Germans Trias i Pujol, Badalona, Spain. 2 The New York Stem Cell Foundation Research Institute, New York, USA. 3 Department of Neurology, Division of Neuromuscular Diseases, Beth Israel Medical Deaconess Center, Harvard Medical School, Boston, USA. 4 Electronic and Biomedical Instrumentation Group, Departament d’Enginyeria Electrònica, Universitat Politècnica de Catalunya, Barcelona, Spain. 5 Laboratory for Stem Cells and Tissue Engineering, Department of Biomedical Engineering, Columbia University, New York, USA. Asymmetric Inheritance of Pax5 May Balance Selfrenewal and Terminal Differentiation of B Lymphocytes Wen-Hsuan W. Lin and Steven L. Reiner Departments of Microbiology & immunology and Pediatrics, College of Physicians & Surgeons, Columbia University, New York, NY 10032 Directed hPSC differentiation to thymic epithelial cells. Rafael Gras, Hans W. Snoeck The Hippo-Yap Pathway Controls Differentiation of Airway Epithelial Progenitors in the Developing and Adult Lung. John E. Mahoney1,2,, Munemasa Mori1, Aleksander D. Szymaniak3, Xaralabos Varelas3, Wellington V. Cardoso1,2 1 Columbia Center for Human Development, Department of Medicine, Columbia University Medical Center. New York, NY 10032; 2 Pulmonary Center, Boston University School of Medicine, Boston, MA 02118 ; 3.Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118. Generation derivatives. of anterior foregut endoderm Ya-Wen Chen, Jingxin Xia, Sarah Xuelian Huang, Megan Skyes, Hans-Willem Snoeck Using Induced Pluripotent Stem Cells to Investigate the Central Nervous System Phenotypes of PraderWilli Syndrome Lisa C. Burnett1,2, Carlos R. Sulsona3, Daniel J. Driscoll3, Dieter Egli1,4, Rudolph L. Leibel1,2,5 1 Columbia University, 2 Institute of Human Nutrition, 3 University of Florida, 4 New York Stem Cell Foundation, 5 Naomi Berrie Diabetes Center. Supported by FPWR Research Grant, FPWR/PWSA PWS Best Idea Grant. Exogenous COL18A1 Restores Retinal Function in a Patient Specific Model of Knobloch Syndrome Huy V. Nguyen, Yao Li, Susanne Koch, Jin Yang, Irene Maumenee, Stephen H. Tsang Electrical Conditioning of Human Embryonic Stem Cell Derived Cardiomyocytes George Eng1,2, Benjamin W. Lee1,2, Lev Protas3, Mark Gagliardi4, Kristy Brown5, Gordon Keller4, Richard B. Robinson3, and Gordana Vunjak-Novakovic1,6 1Department of Biomedical Engineering, Columbia University, New York, NY 10027 2College of Physicians and Surgeons, Columbia University, New York, NY 10032 3Department of Pharmacology, Columbia University, New York, NY 10032 4McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G1L7, Canada 5Department of Pathology and Cell Biology, Columbia University, New York, NY 10032 6Department of Medicine, Columbia University, New York, NY 10032 Timing regulation for efficient differentiation of human induced pluripotent stem cells into cardiomyocytes Dario Sirabella, William Tang & Gordana Vunjak-Novakovic Columbia University Medical Center, Department of Biomedical Engineering and Department of Rehabilitation and Regenerative Medicine, Vanderbilt Clinic 12-234, New York, NY 10032 Generation of pigmented human 3D Skin equivalents containing iPSC-derived melanocytes, keratinocytes and fibroblasts Karl Gledhill, Zongyou Guo, Munenari Itoh, Claire A. Higgins, 6 Columbia Stem Cell Initiative Tapping the Potential of Stem Cells for Human Health Noriko Umegaki, Angela M. Chrstiano networks identifies a synergistic interaction between FOXM1 and CENPF that drives prostate cancer malignancy Site-specific genome editing using TALENs for correction of dominant dystrophic epidermolysis bullosa Antonina Mitrofanova2,*,Alvaro Aytes1,8*, , Celine Lefebvre2,*,^, Mariano J. Alvarez2, Mireia Castillo-Martin9, Tian Zheng7,10, James A. Eastham11, Anuradha Gopalan12, Kenneth J. Pienta13, Michael M. Shen2,3,4,7, Andrea Califano2,5,7, and Cory Abate-Shen1,2,6,7 Satoru Shinkuma1, Angela M Christiano1,2 Departments of Dermatology1 and Genetics and Development2, Columbia University, New York, U.S.A Notch3 signaling identifies a subpopulation of p63negative airway progenitors with features of suprabasal cells in the lung. Munemasa Mori, John E. Mahoney, Hong Zhang, Jining Lu, Jesus Paez-Cortez, James Schwob, Wellington. V. Cardoso Columbia Center for Human Development, Department of Medicine, Columbia University Medical Center. New York, NY 10032; Pulmonary Center, Boston University School of Medicine, Boston, MA 02118 ; University of Massachusetts Medical School, MA; Department of Molecular & Cell Biology, Tufts University, Boston, MA CXCL12-CXCR4 axis supports single Mist1+ gastric stem cell niche and contributes to cancer development. Yoku Hayakawa, Hiroshi Ariyama, Samuel Asfaha, Daniel L. Worthley, Christoph B. Westphalen, Hongshan Wang, Ashlesha Muley, Yagnesh Tailor, Karan Nagar, Michael D. Gershon, Lei Ding, and Timothy C. Wang Innate immune pathways trigger elimination of loser cells during cell competition Cora Bergantiños, Marc Amoyel, Claire de la Cova, Laura A Johnston Department of Genetics and Development Columbia University, New York, NY Expanding the Synthetic Capabilities of the Cell Virginia Cornish’s lab Departments of 1Urology, 2Systems Biology, 3Medicine, 4Genetics & Development, 5Biochemistry and Molecular Biophysics, and 6Pathology and Cell Biology; 7Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, NY 10032; 8Translational Research Laboratory, Catalan Institute of Oncology, Bellvitge Institute for Biomedical Research, Barcelona, Spain 08907; 9Department of Pathology, Icahn School of Medicine at Mount Sinai New York, 10Department of Statistics, Columbia University, New York, NY 10027; Departments of 11Urology and 12Pathology, Memorial Sloan Kettering Cancer Center, New York, NY 10065; 13The University of Michigan Ann Arbor, MI 48109, and the Brady Urological Institute at the Johns Hopkins School of Medicine, Baltimore, MD 21231; *Equal contribution Radioresistant Krt19+ Cells give rise to Lgr5+ Crypt Base Columnar Cells and maintain gut homeostasis following radiation injury in vitro. Ashlesha Muley1, Samuel Asfaha1, Yoku Hayakawa1, Yagnesh Tailor1, and Timothy C. Wang1 Division of Digestive and Liver Diseases, Department of Medicine, Columbia University Transcription factor mediated enhancer-promoter connectivity programs motor neuron identity Michael Closser1, Christopher Reeder2, Yijun Ruan3, David K. Gifford2, Hynek Wichterle1 1 Departments of Pathology and Cell Biology, Neurology, and Neuroscience, Center for Motor Neuron Biology and Disease, Columbia Stem Cell Initiative, Columbia University Medical Center, New York, New York, USA 2 Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, US 3 The Jackson Laboratory for Genomic Medicine, and Department of Genetic and Development Biology, University of Connecticut Syntax of dynamic Isl1 binding sites in maturing motor neurons revealed by ChIP-exo Ho Sung Rhee and Hynek Wichterle A role for Spz in sensing and signaling during cell competition Lale Alpar1, Cora Bergantiños2, Marc Amoyel2, Laura A. Johnston2 1Department of Biological Sciences, Columbia University, New York, NY 2Department of Genetics & Development, Columbia University, New York, NY Cross-species analysis of genome-wide regulatory 7 Probing diversity of stem cell derived motor neurons with single cell expression profiling Chris Tan and Hynek Wichterle Columbia Stem Cell Initiative Tapping the Potential of Stem Cells for Human Health Christopher Henderson1. Proteomics and Personalized Medicine: Protein Profiling Identifies SOD2 as a Mediator of Antioxidative Defense Associated with Susceptibility in Age-Related Macular Degeneration in Patient-Specific Stem Cell Lines Jin Yang1,2,3, Yao Li1,3, Lawrence Chan1,3, Yi-Ting Tsai1,3, WenHsuan Wu1,3, Huy V. Nguyen1, Chun-Wei Hsu, Lewis M.Brown5, Dieter Egli4, Janet R. Sparrow3, Stephen H. Tsang1,3,6 1Bernard & Shirlee Brown Glaucoma Laboratory, Departments of Ophthalmology, Pathology & Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA 2Tianjin Medical University Eye Hospital, TianJin 300384. China 3Edward S. Harkness Eye Institute, Columbia University, New York, NY 10032, USA 4The New York Stem Cell Foundation Laboratory, New York, NY 10032, USA 5Quantitative Proteomics Center, Department of Biological Sciences, Columbia University, New York, NY 10027 6New York-Presbyterian Hospital/Columbia University Medical Center, New York, NY 10032, USA 1Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, Departments of Regenerative Medicine, Pathology, Neurology and Neuroscience, Center for Motor Neuron Biology and Disease (MNC), Columbia Stem Cell Initiative (CSCI), Columbia University Medical Center, New York, USA 2 iPierian, Inc., South San Francisco, California, USA 3The Howard Hughes Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA ApoE influences injury-induced neurogenesis in an isoform-specific manner Sue Hong, MD - Pediatric Critical Care Clinical Fellow PI: Steven Kernie Dept of Pediatrics, Division of Pediatric Critical Care Medicine Specification and subtype diversification of ESCderived spinal interneurons Phuong Hoang, Hynek Wichterle Emerging Roles of Motor Neuron Hyperexcitability in an iPSC Model of ALS Emily Rhodes Lowry1-4, Qinghong Yan2,5,6, Damian J. Williams2,7,8, Derek H. Oakley1-4, Kevin Eggan9, Chaolin Zhang2,5,6, Hynek Wichterle1-4, Christopher E. Henderson1-4. 1Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, 2Center for Motor Neuron Biology and Disease, 3Departments of Pathology and Cell Biology, Neurology, and Neuroscience, 4Columbia Stem Cell Initiative, 5Department of Systems Biology, 6Department of Biochemistry and Molecular Biophysics, 7Columbia Electrophysiology Core, 8 Department of Physiology and Cellular Biophysics, Columbia University Medical Center, New York, NY. 9Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA. Transgenic human ES and IPS cells as a tool to study motor neuron diseases Mathieu Desclaux1, Jackson Sandoe2, Yoon Kim1, Jimmy Hom1, Alejandro Garcia Diaz1, Kevin Eggan2, Hynek Wichterle1 and Christopher Henderson1. 1Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, Departments of Regenerative Medicine, Pathology, Neurology and Neuroscience, Center for Motor Neuron Biology and Disease (MNC), Columbia Stem Cell Initiative (CSCI), Columbia University Medical Center, New York, USA 2The Howard Hughes Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA An SMN-dependent, cell-autonomous survival deficit in human Type 1 SMA ES-derived motor neurons Mathieu Desclaux1, Yoon Kim1, Alejandro Garcia Diaz1, Shila Mekhoubad3, John Dimos2,3, Kevin Eggan3, Hynek Wichterle1 and 8 A stem cell model of the motor circuit reveals distinct requirements of SMN for motor neuron survival and function. Christian M. Simon1,2, Anna M. Janas1,2, Francesco Lotti1,2, Livio Pellizzoni1,2, George Z. Mentis1,2 1. Center for Motor Neuron Biology and Disease, 2. Dept. of Pathology and Cell Biology, Columbia University, NY, 10032 Investigating amyotrophic lateral sclerosis pathophysiology by functional characterization of motor neurons generated from mouse embryonic stem cells John W. Smerdon1, Damian J. Williams4, Emily R. Lowry2,4, Sebastian Thams2, Christopher E. Henderson2,3,4,5, Amy B. MacDermott1,3 and Hynek Wichterle2,3,4,5 1Department of Physiology and Cellular Biophysics, Columbia University, New York, NY. 2Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, New York, NY. 3Department of Neuroscience, Columbia University, New York, NY. 4Department of Pathology, Columbia University, New York, NY. 5 Department of Neurology, Center for Motor Neuron Biology and Disease (MNC), and Columbia Stem Cell Initiative (CSCI), Columbia University, New York, NY. Evaluation of the role of the retinal G proteincoupled receptor (RGR) using patient-specific iPSderived RPE-cells Susanne Koch1,2, Yao Li1,2, Huy V. Nguyen1,2, Yang Jin1,2, Stephen H. Tsang1,2 1Brown Glaucoma Laboratory, Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York Columbia Stem Cell Initiative Tapping the Potential of Stem Cells for Human Health 10032, USA 2Edward S. Harkness Eye Institute, and Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA Prospective identification and purification of quiescent adult neural stem cells from their in vivo niche Paolo Codega, Violeta Silva-Vargas, Alex Paul, Angel R Maldonado-Soto, Annina M DeLeo, Erika Pastrana and Fiona Doetsch Angiogenesis and neurogenesis in the ageing human dentate gyrus Adrienne N. Santiago1, Tanya H. Butt1, Andrew J. Dwork1,3,4, Gorazd B. Rosoklija1,3,7, Victoria Arango1,3, René Hen2,3,5,6, J. John Mann1,3, Maura Boldrini1,3 Divisions of 1Molecular Imaging and Neuropathology and 2Integrative Neuroscience, New York State Psychiatric Institute; Departments of 3Psychiatry, 4Pathology and Cell Biology, 5Neuroscience and 6Pharmacology, Columbia University, New York, NY, USA; 7Macedonian Academy of Sciences & Arts, Republic of Macedonia Identification of direct Nkx2.2 targets involved in the repression of motor neuron identity Abarinov, E. V., Mazzoni, E. O., Closser, M. C., O'Donnell, C., Guo, Y., Balderes, D. A., Morrison, C. A., Gifford, D. K., Wichterle, H. Sussel, L. MicroRNA Regulation of Adult Neural Stem Cells Annina DeLeo, Natalia Molotkova, and Fiona Doetsch Retinal ganglion cell genesis and determination in the binocular circuit subtype Florencia Marcucci, Qing Wang, Takaaki Kuwajima, Sania Khalid, Punita Bhansali, Ilana Rayport, and Carol Mason Departments of Pathology & Cell Biology, Neuroscience and Ophthalmology Gene therapy on patient-specific stem cell lines with MFRP defect Yao Li, Wen-Hsuan Wu, Chun-Wei Hsu, Huy V. Nguyen, Yi-Ting Tsai, Takayuki Nagasaki, Irene H. Maumenee, Lawrence A. Yannuzzi, Quan V. Hoang, Haiqing Hua, Dieter Egli, Stephen H. Tsang. 9