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Chabot College
Fall 2008
Replaced Fall 2011
Course Outline for Microbiology 1
MICROBIOLOGY
Catalog Description:
1 – Microbiology
5 units
Bacteria, fungi, protozoans, parasites, and viruses with an emphasis on their relationship to humans.
Cultivation, control, metabolism, body's defense against disease, microbial genetics, laboratory tests,
and contemporary diseases are discussed. Methods used in the laboratory include staining,
investigation, cultivation, identification of unknowns, and sensitivity testing. Prerequisite: Biology 31,
and Chemistry 30A or Chemistry 1A (both completed with a grade of "C" or higher). Strongly
recommended: Anatomy 1, eligibility for English 1A . 3 hours lecture, 6 hours laboratory.
Prerequisite Skills:
Before entering the course, the student should be able to:
1.
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3.
4.
5.
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8.
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24.
describe and apply the scientific method and how it is used by scientists to further scientific
knowledge;
cite the characteristics and levels of organization exhibited by all living organisms;
use the light microscope and dissecting scope;
describe how cells/specialized cells are structured and function;
describe basic cell metabolism;
describe/contrast, mitosis and meiosis;
describe structure, transmission and expression of genes;
explain the Darwinian concept of evolution as modified by modern scientific knowledge;
describe how the modern (binomial) system names and classifies organisms;
make unit conversions in the metric system;
describe the structure of the atom;
perform calculations using the mole concept;
identify properties of states of matter;
write balanced equations for chemical reactions including those in aqueous solution and
those involving elementary oxidation-reduction;
describe properties of solutions;
use Le Chatelier's principle to predict the qualitative effects of changes in concentration,
temperature and pH on an equilibrium;
perform laboratory experiments in an efficient, safe and purposeful manner;
collect and analyze scientific data;
perform titrations and volumetric analyses;
write balanced chemical equations for oxidation-reduction reactions;
calculate enthalpies of reaction using calorimetry, Hess's law, heats of formation and bond
energies;
describe the bonding in compounds and ions;
explain chemical and physical changes in terms of thermodynamics;
define all concentration units for solutions and solve solution stoichiometry problems.
1
Chabot College
Course Outline for Microbiology 1, page 2
Fall 2008
Expected Outcome for Students:
Upon completion of the course the student should be able to:
1.
describe key aspects of the development of microbiology and its significance to modern
microbiology;
2.
handle microbial cultures in a safe manner;
3.
demonstrate proficiency using the bright field microscope;
4.
list the main characteristics and structural components of viruses, prokaryotic, and
eukaryotic cells, and to discuss their functions;
5.
purify and identify bacterial isolates based on metabolic profiles and microscopic
characteristics;
6.
explain the principles and interpretation of common bacteriological tests;
7.
describe the role of fungi as pathogens and their importance in the environment;
8.
investigate the antimicrobial susceptibility of bacterial isolates;
9.
explain the use of disinfectants, antiseptics, sanitizers and the mode of action of selected
examples;
10.
explain fundamental bacterial genetic principles, and their relevance to disease
development and biotechnology;
11.
describe the main components and functions of the innate and acquire immunity;
12.
describe the conditions that lead to infectious disease development in a population or an
individual, and their control;
13.
develop approaches to control the presentation of infectious diseases.
Course Content (Lecture):
1.
2.
3.
Introduction to microbiology
a.
Areas of study in microbiology
b.
Taxonomy
c.
History
Structure of bacteria
a.
Shapes and arrangements
b.
Flagella
c.
Fimbriae
d.
Capsules
e.
Cell wall
f.
Cell membrane
g.
Cytoplasmic inclusions
h.
Endospores
Cultivation of bacteria
a.
Growth requirements
b.
Classification of media
c.
Obtaining and maintaining cultures
2
Chabot College
Course Outline for Microbiology 1, page 3
Fall 2008
Course Content (Lecture) – continued:
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Metabolism
a.
Enzyme structure and function
b.
Physical factors that influence enzyme activity
c.
Structure of carbohydrates, proteins, lipids, nucleic acids
d.
Metabolism of glucose, proteins, lipids
e.
Aerobic and anaerobic respiration
f.
ATP synthesis and function
g.
Structure of DNA and RNA
h.
Role of mRNA, tRNA, rRNA in protein synthesis
i.
Operon Model
Genetics
a.
Mutations, i.e., chromosome and gene
b.
Genotype, phenotype
c.
Conjugation
d.
Transformation
e.
Transduction
f.
Genetic engineering
Fungi
a.
Structure and reproduction of selected species
b.
Human diseases
c.
Growth requirements
Viruses
a.
Composition
b.
Reproduction
c.
Animal viruses and diseases
Rickettsiae
a.
Structure
b.
Vectors
c.
Human diseases
d.
Methods of diagnosis
Control of Microorganisms
a.
Sterilization, disinfection, antimicrobial agents
b.
Physical factors
c.
Antibiotics and chemotherapeutic agents
Immunology
a.
Nonspecific immunity, i.e., blood cells, lymphatic system, phagocytosis, tissue
factors, complement
b.
Specific immunity, i.e., antigens, antibodies
c.
Clonal selection Theory
d.
T and B lymphocytes and their function
Serological Diagnostic Methods
a.
Agglutination tests
b.
Precipitin tests
c.
Complement fixation tests
d.
Neutralization tests
e.
Radioimmunoassay testing
f.
Fluorescent antibody testing
g.
ELISA
3
Chabot College
Course Outline for Microbiology 1, page 4
Fall 2008
Course Content (Lecture)-continued:
12.
Contemporary Diseases
a.
AIDS
b.
Infectious hepatitis
c.
Dental diseases
Course Content (Laboratory Activities):
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Microscope
Measuring cells
Bacterial morphology and arrangements
Aseptic and isolation techniques
Staining procedures, i.e., simple, gram, acid-fast, spore, capsule
Morphological unknown
Anaerobic culture methods
Carbohydrate fermentation
Respiration reactions
Protein utilization
Differential media
Enterotube II
Bacterial unknown
Serial dilutions and counting bacteria
Tubidometry
Effect of temperature
Effect of U.V.
Antibiotic testing-Kirby Bauer
M.I.C. Testing
Disinfectant testing
Oral hygiene
Evaluation of mouthwashes
Throat culture
Skin culture
Protozoans
Parasitic protozoans
Parasitic flatworm
Parasitic roundworms
Arthropod vectors
Methods of Presentation:
1.
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5.
Lecture and discussion
Observation, collection and analysis of data
Laboratory studies
Charts, models, slides, transparencies
Films
4
Chabot College
Course Outline for Microbiology 1, page 5
Fall 2008
Assignments and Methods of Evaluating Student Progress:
1.
Typical Assignments
a.
Lecture. Compare and contrast the cellular components of Gram positive and
Gram negative bacteria. Discuss how these cells respond to antimicrobials in the
context of their cellular structure
b.
Laboratory. Utilizing data gathered from the epidemiology exercise determine the
rate of hand contact transmission of Serratia marcescens in a population. Plot the
data in a chart and determine the prevalence of infection. Given an unknown
culture determine by microscopy and biochemical tests the identity of the
organisms. Proper use of aseptic technique and microscope use is assessed as
well
2.
Methods of Evaluating Student Progress
a.
Examinations
b.
Quizzes
c.
Laboratory practical
d.
Bacterial unknowns
e.
Laboratory reports
f.
Final exam
Textbook(s) (Typical):
Microbiology, 7th edition, Tortora, McMillian, 2007
Laboratory Experiments in Microbiology, Johnson, Pearson Benjamin Cummings, 2007
Special Student Materials:
1.
2.
3.
Lab coat or apron
Marking pen
Colored pencils
CE
Revised 9/2007
5
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