Download FINAL EXAMINATION

FINAL EXAMINATION
JANUARY 2014 SEMESTER
COURSE
COURSE CODE
DURATION
FACULTY
PROGRAMME
LECTURER
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CLINICAL MICROBIOLOGY I
MLD 2143
2 HOURS 30 MINUTES
BIOMEDICAL AND HEALTH SCIENCES
DIPLOMA IN MEDICAL LABORATORY
TECHNOLOGY
: MR. MOHD AMIR ABDULLAH
INSTRUCTIONS TO CANDIDATES
This paper consists of three (3) sections:
Section A – 30 questions (multiple choice questions)
Section B – 3 questions (short answer questions)
Section C – 3 questions (essay questions)
Answer all questions in Section A and B and only two (2) questions in Section C.
All answers of:
Section A must be written in OMR objective paper provided.
Section B must be written in answer booklet provided.
Section C must be written in answer booklet provided.
The answer for each question must start on a new page.
Formula and table are provided at the end of question paper.
Candidates are allowed to use calculator.
Candidates are NOT ALLOWED to bring any unauthorized items into the exam hall
except with the permission of your invigilator.
Do Not Open the Question Paper Until Instructed
This Question Paper Consists of Nine (9) Printed Pages
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PART A: MULTIPLE CHOICE QUESTIONS
(30 MARKS)
Choose ONE best statement to answer the questions.
1. The bacterial species that has cocci in chain is
(A)
(B)
(C)
(D)
Streptococcus spp.
Pneumococcus spp.
Staphylococcus spp.
Corynebacterium spp.
2. A known culture of Salmonella typhi appears pink in Gram stain and bacilli in
shape. This result could be
(A)
(B)
(C)
(D)
incorrect as the bacteria are not bacilli
correct as the bacteria is Gram negative
incorrect as the bacteria is Gram positive
correct because the bacteria show variability in colour and shape
3. In slide preparation for staining, what is the process that allows the bacterial cells
to adhere to the glass slide?
(A)
(B)
(C)
(D)
fixation
smearing
counterstaining
decolourization
4. By definition, a bacterial isolate is Staphylococcus aureus if it
(A)
(A)
(B)
(C)
ferments glucose
produces coagulase
produces free catalase
produces hemolysis in blood agar
5. Which of the following statement is TRUE?
(A)
(B)
(C)
(D)
Staphylococcus pyogens is resistant to 5µg bacitracin.
Streptococcus viridans produces gamma hemolysis on blood agar.
Catalase test is used to differentiate Streptococcus pneumonia from
Streptococcus agalactiae.
Novobiocin test is used to differentiate Staphylococcus epidermidis from
Staphylococcus saprophyticus.
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6. Staphylococcus aureus can be differentiated from Streptococcus pyogens using
the following test
(A)
(B)
(C)
(D)
motility
catalase
oxidase
coagulase
7. The Lancefield grouping of Streptococcus pyogenes is a
(A)
(B)
(C)
(D)
Lancefield Group A
Lancefield Group B
Lancefield Group C
Lancefield Group D
8. Which of the following is TRUE about Neisseria gonorrhoea?
(A)
(B)
(C)
(D)
It is a Gram positive
It can produce oxidase
It is a bacillus in shape
It metabolizes maltose and sucrose
9. Culture on Modified Thayer Martin (MTM) medium of a vaginal swab produced
several colonies of Gram-negative diplococci that are oxidase positive. Given the
following carbohydrate reactions. In the table 1, select the most likely causative
organism.
Carbohydrates Reaction
Glucose
Sucrose
Lactose
Maltose
Result
Positive
Negative
Negative
Positive
Table 1
(A)
(B)
(C)
(D)
Salmonella enteric
Moraxella catarrhalis
Neisseria meningitidis
Corynebacterium diphteriae
10. Tellurite medium is normally used to isolate
(A)
(B)
(C)
(D)
Staphylococcus aureus
Neisseria gonorrhoeae
Haemophilus parainfluenzae
Corynebacterium diphtheriae
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11. Which of the following medium is NOT a transport media for Vibrio cholera?
(A)
(B)
(C)
(D)
Cary-Blair Medium
Loeffler’s serum slope
Alkaline peptone water
Venkatraman-Ramakrishnan medium
12. A bacteria which can be cultured on Skirrow’s medium, Gram-negative bacilli,
actively motile, oxidase positive and identified by failure to grow at 25 °C is
(A)
(B)
(C)
(D)
Vibro cholerae
Echerichia coli
Campylobacter jejuni
Propionibacterium granulosum
13. Which of the following organisms requires "X (haemin)" factors for growth?
(A)
(B)
(C)
(D)
Haemophilus ducreyi
Haemophilus influenza
Haemophilus parainfluenzae
Haemophilus parahaemolyticus
14. The colonies that exhibit satellite formation near colonies of staphylococci belong
to
(A)
(B)
(C)
(D)
Shigella boydii
Streptococcus pyogens
Haemophilus influenza
Neisseria gonorrhoeae
15. All Enterobacteriaceae share all of the following characteristics EXCEPT
(A)
(B)
(C)
(D)
Gram negative
ferment glucose
oxidase negative
reduce nitrates to nitrites
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16. Which of the following is FALSE about Proteus vulgaris?
(A)
(B)
(C)
(D)
A Gram-negative bacilli
A non lactose fermenting organism
A non glucose fermenting organism
An organism can produced swarming growth on blood agar medium
17. The Shigella boydii
(A)
(B)
(C)
(D)
is motile
can produce gas
can produce hydrogen sulfide
is a glucose fermenting organism
18.
An organism isolated from urine is non lactose fermenting, Gram-negative
bacilli, motile, capsulated and appeared as swarming growth colonies on blood
agar.
Figure 1
What is the presumptive identification of the organism described in figure 1?
(A)
(B)
(C)
(D)
Proteus mirabilis
Moraxella catarrhalis
Listeria monocytogens
Klebsiella pneumoniae
19. Which of the following is the characteristic genus Mycobacterium?
(A)
(B)
(C)
(D)
is a cocci
is a motile
is an anaerobic
has high cell wall lipid content
20. Dorset Egg and Lowenstein-Jensen medium are enriched media which is used to
isolate
(A)
(B)
(C)
(D)
Bacillus anthracis
Salmonella bongori
Actinomyces israelii
Mycobacterium tuberculosis
.
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21. The growth of Mycobacterium tuberculosis in the laboratory will normally take
between
(A)
(B)
(C)
(D)
4-6 days
4-6 hours
4-6 weeks
4-6 months
22. Which of the following stain is required to prepare smear of sputum suspected of
Mycobacterium leprae?
(A)
(B)
(C)
(D)
Indian ink
Gram stain
Ziehl-Nielson
Acid fast stain
23. Which of the following organisms is the aetiological agent for acne?
(A)
(B)
(C)
(D)
Actinomyces species
Staphylococcus species
Peptotreptococcus species
Propionibacterium species
24. Treponema pallidum can cause
(A)
(B)
(C)
(D)
syphilis
meningitis
otitis media
tuberculosis
25. Which of the following characteristic describe the Leptospira interrogans?
(A)
(B)
(C)
(D)
non motile
cocci bacteria
Gram-positive
seen under Dark Ground Microscope
26. Which disease is detected by the Wasserman complement fixation test?
(A)
(B)
(C)
(D)
Leprosy
Syphilis
Diarrhoea
Tuberculosis
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27. Which of the following is TRUE about Mycoplasma pneumoniae?
(A)
(B)
(C)
(D)
It can be stained with Dienes stain
It’s normal flora in the human lungs
It can be transmitted to by insect bites
It cannot hemolyze guinea pig red blood cells
28. Which of the following staining techniques is commonly used to identify
Chlamydia trachomatis?
(A)
(B)
(C)
(D)
Gram stain
Diene’s stain
Giemsa stain
Acid-fast stain
29.
When stained this organism is large, gram positive rods with square end, found
in chains, non motile and has antiphagocytic capsule composed of D-glutamate.
It’s causes anthrax.
Figure 2
What is the presumptive identification of the organism described in figure 2?
(A)
(B)
(C)
(D)
Bacillus antracis
Campylobacter jejuni
Neisseria gonorrhoeae
Listeria monocytogenes
30. Which of the following staining techniques is commonly used to identify
Corynebacterium diphtheriae?
(A)
(B)
(C)
(D)
Albert stain
Acid fast stain
Warthin-starry silver stain
Haematoxylin-Eosin stain
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PART B: SHORT ANSWER QUESTIONS
(30 MARKS)
Answer ALL questions.
1. a. Draw and name morphology of bacteria according to shape.
(6 Marks)
b. Briefly explain the general steps in diagnostic isolation and
identification of bacteria.
(4 Marks)
2. a. Determine FOUR (4) basic biochemical tests.
(2 Marks)
b. Briefly explain the purpose and the principle of FOUR (4)
basic biochemical tests.
(8 Marks)
3. a. Determine the general characteristics of the Escherichia Coli.
(5 Marks)
b. Briefly describe the laboratory diagnosis to detect Klebsiella
pneumoniae.
(5 Marks)
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PART C: ESSAY QUESTIONS
(40 MARKS)
Answer TWO (2) questions only.
Question 1
Discuss and draw the schematic diagram for the identification Streptococcus species
and describe the appearances of alpha, beta and gamma haemolysis on blood agar.
Give ONE (1) example of an organism for each of the haemolysis.
(20 marks)
Question 2
Describe the general characteristics, method of acquiring and laboratory investigation
of Salmonella enterica.
(20 marks)
Question 3
Describe important properties of Haemophilus influenzae including staining and
morphological characteristics and laboratory investigation.
(20 Marks)
END OF QUESTION PAPER
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