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Depolymerization of Heparin/HS
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Application Overview
Heparin and heparan sulfate (HS) glycosaminoglycans are linear sulfated
polysaccharides located on cell-surface membranes and in extracellular matrices in
virtually all animal tissues. Heparin and HS have been implicated in cell-biological
processes, cell adhesion and regulation of enzymatic catalysis (1). HS chains have
been shown to interact with a variety of growth factors, chemokines, extracellular
matrix proteins, and enzymes, including antithrombin, fibroblast growth factors, and
vascular endothelial growth factor (2) as shown by choosing the tab below. Heparin
has been widely used as an anticoagulant drug (3,4), and it has been shown to
regulate cellular process by binding, stabilizing and activating various growth factors
(5).
References
1. Fritz, T., et al (1994) J Biol Chem 269(46), 28809-28814. PMID: 7961837
2. Linhardt, R. J., et al. (1990) Biochemistry 29(10), 2611-2617. PMID: 2334685
3. Linhardt, R. J. and Gunay, N. S. (1999) Semin. Thromb. Hemost. 25 Suppl. 3, 5-16. PMID:
10549711
4. Casu, B., et al. (2002) Biochemistry 41(33), 10519-10528. PMID: 12173939
5. Knudsen, C. B., Knudsen, W. (2001) Semin. Cell. Dev. Biol. 12, 69-78. PMID: 11292372
6. Ilan N, Elkin M, Vlodavsky I. (2006) Int. J. Biochem. Cell. Biol. 38(12), 2018-39. PMID: 16901744
7. Rosen SD, Lemjabbar-Alaoui H. (2010) Expert Opin. Ther. Targets. 14(9), 935-49. PMID: 20629619
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HS chain interactions
Legal Information
Depolymerization of Heparin/HS includes these
areas of focus:
MS Analysis of GAGs
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New
England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require
the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at
[email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or
diagnostic purposes in humans or animals.
HS chain interactions
Membrane bound proteoglycan (syndecan) showing heparan sulfate chains binding to vascular endothelial growth
factor (VEGF). Highly sulfated domains bind free VEGF, creating a morphogen gradient, and thus facilitating
binding to its receptor in the plasma membrane. Heparin-bound VEGF is released upon desulfation by sulfatases.
Alternatively, active heparin fragments are shed by heparinases. These mechanisms are critical during blood vessel
morphogenesis, particularly in tumor proliferation (6,7).
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