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A Geographical Comparison Concerning the Seroprevalence of Toxoplasma gondii in Domestic Cats of York and Lancaster Counties Justin Klinedinst and David Singleton Department of Biological Sciences, York College of Pennsylvania Introduction • Domestic and feral cats are the only species that are able to excrete the infectious sporulated form of the parasite termed oocysts (Dubey 2004). • The infectious disease caused by the parasite is termed Toxoplasmosis, a zoonosis that can also be contracted by humans through the ingestion of undercooked meat and contaminated water. •Pregnant women are extremely susceptible to the parasite, which is congenital and prevalent in many areas across the United States and throughout the world. Because of its almost ubiquitous nature, Toxoplasma gondii is of medical importance due to its capability to cause congenital disease or even abortions (Wong et 0.6 * 0.4 0.2 York Collect felid serum samples from local veterinary hospitals of York and Lancaster Lancaster Figure 2. A depiction of the percentage of T. gondii positive serum samples for outdoor cats of York (n=28) and Lancaster (n=7) counties. 0.3 0.2 0.1 0.0 York Lancaster Figure 3. A depiction of the percentage of T. gondii positive serum samples for indoor cats of York (n=31) and Lancaster (n=16) Counties. Conduct ELISA assay on collected serum samples Measure the absorbance to detect T. gondii postive serum samples Results H0: There will be no difference in T. gondii seroprevalence when comparing cats from York and Lancaster Table 1. Percentage of positive T. gondii serum samples Conclusions H0: Rejected, a difference was revealed between the two counties H1: A higher seroprevalence was observed in outdoor cats as opposed to indoor cats for York County but not Lancaster County. H2: Rejected, from the data collected a higher prevalence was associated with York County cats as opposed to Lancaster County H1: Outdoor Cats of both York and Lancaster will have a higher seroprevalence than indoor cats H2: Cats from Lancaster will show a higher seroprevalance of T. gondii than cats from York * 0.0 al. 1994). Hypotheses Percentage Positive for T. gondii Methods Percentage Positive for T. gondii • Toxoplasma gondii (T. gondii) is an intracellular coccidian parasite that employs felids as its primary hosts and other vertebrates such as humans, livestock, and mice as intermediate hosts (Morisaki et al. 1995). 0.8 Literature Cited Figure 1. Results from the Indirect Enzyme-Linked Immunosorbent Assay (ELISA). The yellow colored wells depict positive T. gondii serum samples. Dubey, J. P. 2004. Toxoplasmosis- a waterborne zoonosis. Veterinary Parisitology. (126):57-72. Morisaki, J. (et al.). 1995. Invvasion of Toxoplasma gonodii occurs active penetration of the host cell. Journal of Cell Science. (108): 2457-2464. Wong, S. (et al.). 1994. Toxoplasmosis in Pregnancy. Clinical Infectious Diseases. (18)6: 853-861.