Download IDEXX IBR gE Ab Test Differentiates between marker

IDEXX IBR gE Ab Test
Test With Confidence™
Differentiates between marker-vaccinated
and BHV-1-infected
The IDEXX IBR gE Ab Test is an enzyme
immunoassay for the detection of bovine
herpesvirus-1 specific gE antibodies in
bovine serum, plasma and milk samples.
Infectious bovine rhinotracheitis (IBR) is a
highly contagious, infectious disease that
is caused by bovine herpesvirus-1 (BHV-1).
Typical clinical signs associated with infection
include respiratory disease, but the virus can
cause conjunctivitis, vulvovaginitis, abortions,
encephalitis and balanoposthitis. The transition
from primary manifestions of infection to a latent
stage of persistency is often the source of spread
after virus reactivation. Confirmation of exposure to BHV-1 is facilitated by
a measurement of antibody.
When used in IBR control programs together with gE-deleted vaccines, the
IDEXX IBR gE Ab Test can be an effective differential test for distinguishing
naturally infected animals from vaccinated animals.
•Blocking ELISA to screen for infected or traditionally vaccinated cattle
•Differentiation of infected animals from vaccinated animals in combination
with gE-deleted marker vaccine
•Serum, plasma and milk applications
•6 -plate (strips) and 30-plate (solid) formats, ready-to-use reagents
•Easy to automate
•Compatible with xChek* software
Specificity: Dutch bovine population (n=1730)
Sensitivity: Temporal sera
IBR gE-deleted, multivaccinated Dutch bovine
Seroconversion post-IBR infection
1.4
600
Cutoff
cow 2
cow 3
cow 4
1.0
400
300
0.8
0.6
200
0.4
100
0.2
0
cow 1
1.2
S/N ratio
Frequency
500
0.60
0.75
0.90
1.05
1.20
0
1
3
4
5
6
7
8
9
10
11
12
13
14
Days post-infection
S/N value
Average S/N = 1.02; Standard deviation = 0.075
Specificity = 99.83% (95% CL 99.7–100%)
Interpretation of results: S/N ratio
> 0.7 negative; = 0.6–0.7 suspect; < 0.6 positive
Conclusion: The IDEXX IBR gE Ab Test shows
specificity greater than 99% using samples from
characterized, multivaccinated herds.
Conclusion: The IDEXX IBR gE Ab Test detected gE antibodies at days
9 to 10 post-infection.
Source: Infectious Bovine Rhinotracheitis Virus (BHV-1) gE Antibody Test
Kit, Validation studies of a new sample diluent reagent, summary report.
IDEXX Laboratories, April 2001.
Source: Experiences with the second generation of IDEXX IBR gE Ab ELISA test.
Proceedings from: Ballagi A, Holmquist G, Palmquist S, Schroeder C, Tonelli Q, 2nd.
International Symposium on BHV-1 Eradication, 1999, Stendal, Germany.
21
IDEXX IBR gE Ab Test
Sensitivity
Sample Characterization
IBR Antibody (Ab) Status—
Expected Result
IDEXX IBR gE Ab Result
(S/N)
R1
1:64 dilution of a serum positive for Ab to IBR in a pool of negative sera
++
0.29 (+)
R2
1:2 dilution of R1 in a pool of negative sera
+
0.47 (+)
R3
1:2 dilution of R2 in a pool of negative sera
+
0.61 (+/–)
R4
Calf that received colostrums from a IBR gE-deleted vaccinated cow
–
0.87 (–)
R5
Calf that received colostrums from a IBR gE-deleted vaccinated cow
–
0.88 (–)
R6
Calf that received colostrums from a IBR gE-deleted vaccinated cow
–
0.91 (–)
R7
Bull, vaccinated with IBR gE-deleted vaccine
–
0.86 (–)
Sample
R11
Reference sample from France
+++
0.05 (+)
R12
Latent IBR infected cow after reactivation of infection
+
0.24 (+)
R14
Latent IBR infected cow after reactivation of infection
+
0.22 (+)
R17
IBR gE-deleted vaccinated and IBR infected calf
+
0.36 (+)
R18
IBR gE-deleted vaccinated and IBR infected calf
+
0.46 (+)
0.30 (+)
R19
IBR gE-deleted vaccinated and IBR infected calf
+
R20
IBR gE-deleted vaccinated and IBR infected calf
+
0.48 (+)
R23
IBR gE-deleted vaccinated and IBR infected calf
+
0.15 (+)
R31
IBR negative and nonvaccinated cow
–
0.86 (–)
R32
IBR negative and nonvaccinated cow
–
0.86 (–)
Sample R1–R32: Results of the IDEXX Reference Lab participating at the IBR Ringtrial 2001. Dr. M. Beer, Federal
Research Center, Riems, Germany.
–
+
++
+++
++++
negative
weak positive
positive
strong positive
very strong positive
Interpretation of results:
S/N value
> 0.7 negative (–)
= 0.6–0.7 suspect (+/–)
< 0.6 positive (–)
Conclusion: In this characterized sample population, the IDEXX IBR gE Ab Test showed 100% agreement with the expected
results, and did not react with samples of cattle vaccinated with gE-deleted vaccine.
For more information about the IDEXX IBR gE Ab Test, please contact your
IDEXX representative or visit idexx.com/ibr.
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