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AP BIOLOGY
CELLULAR ENERGETICS
ACTIVITY #4
NAME_____________________
DATE___________HOUR_____
PHOTOSYNTHESIS
SUMMARY E QUATION
6CO2 + 12H2O + light à C6H12O6 + 6O2 + 6H2O
6CO2 + 6H2O + light à C6H12O6 + 6O2
CO2 + H2O + light à CH2O + O2
(emphasizes production of glucose 1 carbon at a time)
SITE OF P HOTOSYNTHESIS -- PLANTS
Cellular Energetics Activity #4 page 1
SITE OF P HOTOSYNTHESIS – PROKARYOTES
Lack chloroplasts
Chlorophyll built into plasma membrane
STRUCTURE OF CHLOROPLASTS
Cellular Energetics Activity #4 page 2
OVERVIEW OF PHOTOSYNTHESIS
CO2
Light
ADP
+ P
Light Rxns
(thylakoids)
ATP
NADP+
Calvin
Cycle
(stroma)
NADPH
H2O
O2
Light Rxns
• Take e- from H2O
• Use light energy to
boost e- to higher
energy level
• Use some energy to
make ATP
• Add high energy eto NADP+
Cellular Energetics Activity #4 page 3
Calvin Cycle
• AKA Dark Rxn
• Uses ATP produced in
light rxn
• Takes high energy efrom NADPH and adds
them to CO2
• Produces sugar 1 carbon
at a time
CH2O
LIGHT REACTIONS – C YCLIC E LECTRON F LOW
1o electron
acceptor
2e-
Fd
Pq
H+ gradient
chemiosmosis
Cytochrome
complex
P700
Rxn
Center
ATP
Pc
Photosystem
Few 100 pigment molecules (chlorophyll a, b, & carotenoids)
Reaction center
Chlorophyll a molecule
Bound to specific proteins
Closer to 1 o electron acceptor
Antenna pigments absorb photons and pass to rxn center
Cellular Energetics Activity #4 page 4
LIGHT REACTIONS – NONCYCLIC E LECTRON FLOW
Uses downward slide of
e- to create H+ conc.
gradient; H+ gradient
used to make ATP
1o eacceptor
1o eacceptor
Fd
2e-
Pq
NADP+
reductase
Adds 2eand H+ to
NADP+
2eCytochrome
Complex
Pc
H2O
2e2H+
+
½O2
NADP+
P680 photosystem II
Cellular Energetics Activity #4 page 5
P700 photosystem I
NADPH
C ALVIN C YCLE Location = Stroma
RuBP = ribulose biphosphate
Rubisco = RuBP carboxylase
PGA = phosphoglycerate
DPGA = diphosphoglycerate
PGAL = phosphoglyceraldehyde
glyceraldehydes phosphate
RuP = ribulose phosphate
Cellular Energetics Activity #4 page 7
PROBLEM – P HOTORESPIRATION
If [O2] > [CO2] in leaves
Rubisco fixes O2 instead of CO2
5-C compound produced
5-C à 1 PGA enters Calvin Cycle
1 glycolate (2-C) exits chloroplasts and enters
peroxisomes
Decreases productivity
Fostered by hot, dry, bright days
C4 PLANTS
• Fix CO2 as 4C compound
• Segregate CO2
fixation from
Calvin cycle
• Acts as CO2
pump
• PEP
carboxylase
has lower
affinity for O2
than rubisco
• Adaptation in
hot regions
with intense
light
Cellular Energetics Activity #4 page 8
CAM PLANTS
Crassulacean Acid Metabolism
Cellular Energetics Activity #4 page 9