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May 2009 Freeze-thaw stability of emulsions – model system ”mayonnaise” Diploma project 30p Background Källbergs Industri AB produces and markets speciality type of products for mayonnaise based on egg yolk. During later years the sales have increased considerably and products are exported to a number of countries such as Russia, Ukraine, Bulgaria, Great Britain and Kazakhstan. An important property for a mayonnaise is if it can withstand freezing followed by thawing without that the emulsion breaks. Goal The project goal is to create an increased knowledge about freeze-thaw stability of mayonnaise. Description of the problem Mayonnaise is generally known as an oil-in–water emulsion. In order to prolong the shelf life or due to practical circumstances during transport is the mayonnaise often frozen. During the freeze-thaw procedure is the emulsion destabilised and partial or complete separation in oilrespectively water phase often occurs. The freeze-thaw stability of the emulsion is affected of mainly two mechanisms: - The successive increase of dissolved substances in the liquid water phase. High concentrations of salts and/or sugars then changes the conditions at the surface between the oil and water phase. - The dispersed oil droplets are affected by mechanical stress when the water phase expands during freezing. Due to this there is an increased risk that the adsorbed layer surrounding the individual oil droplet ruptures. Methods: During the project the influence on freeze-thaw stability will be invesitgsted with respect to some selected process and formulation parameters. Potential parameters to evaluate are: - quality and origin of the vegetable oil - quality of egg yolk (natural composition or enzymatically modified) - quality of the emulsion (size and distribution of the oil droplets) - influence of salts – ion strength - ratio between water and oil phase - ingredients as emulsifying OSA-starch, hydrocolloids and milk proteins, etc Analytical methods; Measurements of drop size distribution, rheological measurements, degree of separation after thawing, separation after centrifugation etc. The thesis subject can be food technology, biotechnology, pharmaceutical technology or another similar subject relevant for the student. The starting date can be anytime. If you are interested contact: [email protected] or [email protected]