Download Human Protein Kinase B alpha (Akt1/PKBalpha)

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

Document related concepts
no text concepts found
Transcript 
xxxxxx xxxxxx
Xxxxx Xxxxxxx Xxxxxx X xxxxx (Xxxx/XXXx)
Catalog ref. xxx-xx
Referred to XXX n°XXXXX
Purpose
Evaluation of the effects of compounds on the activity of the human
XXXX quantified by measuring the xxxxxxxxxxxxxxx of the substrate
xxxxxxxx-XXXXXXXXXXXXXXXXX using a xxxxx xxxxxxxxxxx enzyme
expressed in insect cells and the XXXX detection method.
Experimental protocol
The test compound, reference xxxxxxxx or water (control) are
preincubated for X min at room temperature with the xxxxxx (XX xx) in a
buffer containing XX xx Xxxxx XXXX (pH X,X), X xx XXXXX, X Xx XXX, XX xX
XxxXxx and X.XXXX Xxxxx XX.
Thereafter, the reaction is initiated by adding XX xX of the xxxxxxxxx
xxxxxxxx-XXXXXXXXXXXXXXXXX and X xX ATP, and the mixture is incubated
for XX min at room temperature.
For control xxxxx measurements, the xxxxxx is omitted from the reaction
mixture.
Following incubation, the reaction is stopped by adding XX xX XXXX.
The xxxxxxxxxxxx acceptor (XXXXX-labeled xxxxxxxxxxxxx) and the
xxxxxxxxxxxx donor (xxxx-xxxxxxx-XXXX xxxxxxxx labeled with xxxxxxxx
cryptate) are then added.
After XX min, the xxxxxxxxxxxx transfer is measured at =xxx nm, xxx=XXX
and xxx=XXX nm using a microplate reader (Xxxxxxxx, XXX
The xxxxxx xxxxxxxx is determined by xxxxxxxx the xxxxxx measured at
XXX xx by that measured at XXX xx (ratio).
The xxxxxxx are xxxxxxxxx as a xxxxxxx inhibition of the control xxxxxx
activity.
The standard xxxxxxxxxx reference compound is xxxxxxxxxxxxx, which is
tested in each experiment at several xxxxxxxxxxxxxx to xxxxxx an
xxxxxxxxxx xxxxx from which its XXxx value is calculated.
Bibliographic reference
BARNETT, S.F, DEFEO-JONES, D., FU, S., HANCOCK, P.J., HASKELL, K.M., JONES, R.E., KAHANA,
J.A., KRAL, A.M., LEANDER, K., LEE, L.L., MALINOWSKI, J., McAVOY, E.M., NAHAS, D.D.,
ROBINSON, R.G. and HUBER, H.E. (2005), Identification and characterization of pleckstrinhomology-domain-dependent and isoenzyme-specific Akt inhibitors, Biochem. J, 385: 399.
General information
August 10, 2005
Assay volume and format:
XX xx in XX-xxxx plate
Compound addition:
xxxxxx concentrated xxxxxxxx in XXX
Maximum tolerable XXXX concentration: XX
Xxxxxxxxxxxx compatible:
Yes 
No 
XXX xxxxxxxxxx:
Yes 
No 
[email protected]
www.xxxxx.com
Related documents