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Figure 14. Programmed Cell Death Defect A. During development, in wild type embryos more cells appear especially in the neuronal system than are needed. In late embryogenesis, these dispensable cells die, by programmed cell death. Here an embroy stained for 2 minutes with acridinorange is shown. Acridinorange is intercalating into the DNA, and marks dying cells with DNA fragments. Intact cellular DNA does not stain. B. MBT develops a brain tumor in third instar larvae. In MBT embryos, programmed cell death is absent. Shown is a 5 minutes stained embryo. Animals were kept at 29°C. Isolde Riede et al. Switch the Tumor Off: From Genes to Amanita Therapy. American Journal of Biomedical Research, 2013, Vol. 1, No. 4, 93-107. doi:10.12691/ajbr-1-4-5 © The Author(s) 2013. Published by Science and Education Publishing.
