Download Orbitrap Elite Hybrid Mass Spectrometer

Thermo Scientific Orbitrap Elite
Hybrid Mass Spectrometer
High-Field Orbitrap
Creating new possibilities
Ultrahigh resolution • Faster scanning
Higher sensitivity • Complementary fragmentation
The highest resolution,
most versatile Orbitrap instrument ever
The Thermo Scientific Orbitrap Elite hybrid mass spectrometer raises the bar for MS and
MSn performance and versatility by combining our premier dual-pressure linear ion trap
mass spectrometer with a novel high-field Orbitrap™ mass analyzer. The system delivers
greater than 240,000 resolution (FWHM), increased sensitivity, high scan speeds and a
larger dynamic range.
High-field Orbitrap mass analyzer for
Higher sensitivity for complex samples
ultrahigh resolution
Innovations in the pre-amplifier electronics improve sensitivity. Paired
The high-field Orbitrap mass analyzer geometry and advanced signal
with the ultrahigh resolution, they provide more comprehensive data
processing technologies enable resolution of >240,000 FWHM,
and a deeper look into complex samples.
a 4x increase over previous technology. This ultrahigh resolution
is especially useful when analyzing samples of high complexity
Fragmentation options increase versatility
and targeting analytes of low abundances in applications such as
In addition to traditional collision-induced dissociation (CID), the
proteomics, metabolomics and lipidomics.
Orbitrap Elite mass spectrometer features higher-energy collisional
dissociation (HCD) that provides superior fragmentation and
Faster scanning for UHPLC compatibility
higher-quality MS/MS spectra for many types of samples. Optional
The high-field Orbitrap geometry and advanced signal processing also
electron transfer dissociation (ETD) preserves labile post-translational
provide an up to 4x improvement in scan speed, making the Orbitrap
modifications of proteins. Intelligent data-dependent decision tree
Elite system more compatible with narrow chromatographic peaks
(DDDT) logic can combine multiple fragmentation techniques in a
from UHPLC separations.
single analysis to significantly improve proteome coverage.
Electrospray
Ion Source
Square Quadrupole
with Neutral
High-Pressure
LowBlocker
S-Lens
Octopole
Cell
Pressure Cell
Quadrupole
Mass Filter
C-Trap
HCD Collision Cell
Transfer Multipole
Reagent Ion Source
e-
New Detection
Electronics
ETD Option
New High-Field Orbitrap
Mass Analyzer
Reagent 1
Heated Inlet
Reagent 2
Heated Inlet
3
The Orbitrap Elite mass spectrometer can analyze complex samples at a greater depth
than ever before, helping scientists meet the most demanding challenges in proteomics,
metabolomics, lipidomics and metabolism.
A quantum leap in Orbitrap MS performance:
Key features of the Orbitrap Elite system:
• High-field Orbitrap mass analyzer for higher mass resolution and
higher scan speed
• Advanced signal processing for higher resolution
• Improved ion optics for efficient ion injection into the Orbitrap
• New Orbitrap image current pre-amplifier for higher sensitivity
• Velos Pro linear ion trap generation II ion optics with neutral blocking
technology for increased robustness
A new standard of performance
for comprehensive proteomics
Orbitrap-based mass spectrometers are an essential tool in many
proteomics labs. The enhanced power of the Orbitrap Elite instrument enables
new standards of performance for multiple proteomics applications.
Top-down analysis
Protein ID
Maximum resolution of > 240,000 FWHM and complementary
Faster scanning for more data points across a peak, combined with high
fragmentation modes facilitate better top-down protein identification and
resolution and superior spectral quality, yields more protein identifications in
characterization.
a single analysis.
PTM analysis
Quantitative proteomics
Available electron transfer dissociation (ETD) preserves labile side chains while
Superior resolution and selectivity of the Orbitrap mass analyzer for both full-
creating fragmentation throughout the peptide backbone for better analysis of
scan MS and MSn enhance relative quantitation analyses such as those using
post-translational modifications (PTMs).
stable isotope labeling or isobaric mass tags.
Intact protein analysis
47+ charge state of yeast enolase showing resolution of >100,000 FWHM at m/z 1,000
993.9134
R=109564
Relative Abundance [%]
80
994.0827
R=107785
993.8706
R=107582
60
50
40
993.8284
R=113310
993.7860
R=106257
994.1249
R=106063
994.1670
R=107915
30
Relative Abundance [%]
994.0401
R=107426
90
46642.586
80
993.9770
R=110221
100
70
100
60
40
20
0
46620
46650
Mass, Da
Deconvoluted spectrum of yeast enolase
(monoisotopic mass 46642.214 Da).
20
10
0
993.3 993.4 993.5 993.6 993.7 993.8 993.9 994.0 994.1 994.2 994.3 994.4 994.5 994.6 994.7 994.8
m/z
Analysis of intact yeast enolase (46.64 kDa) on the Orbitrap Elite hybrid MS demonstrates the superior resolution that makes it the new standard for proteomics applications.
5
Orbitrap Elite
LTQ Orbitrap Velos
1000
Number of Identified Proteins
The superior resolution and spectral quality, as well as the higher scan
speed of the Orbitrap Elite instrument, increase proteome coverage in
complex samples even with very low sample amounts. Here, analysis
of the E. coli proteome labeled with Thermo Scientific Pierce Tandem
Mass Tags demonstrates significant improvement over the already
outstanding performance of the previous-generation Thermo Scientific
LTQ Orbitrap Velos hybrid MS.
800
933
873
803
713
694
600
563
400
200
0
20 ng
80 ng
200 ng
Top-down analysis
S G R G K G G K G L G K G G A K R H R K V L R D N I
ETD
Q G I T K P A I R R L A R R G G V K R I S G L I Y E E T
HCD
R G V L K V F L E N V I R D A V T Y T E H A K R K T V
T A M D V V Y A L K R Q G R T L Y G F G G
HCD
Relative Abundance [%]
100
843.9602
R=158115
z=2
80
60
40
20
0
385.7177
R=197410
z=2
762.4287
R=158642
z=2
500
ETD
Resolution
~ 200,000 FWHM at m/z 400
Resolution
> 100,000 FWHM at m/z 1,000
80
60
600.3526
R=185866
z=2
537.9891
R=189201
z=3
40
1001.0440
R=115639
z=2
1000
m/z
100
Resolution
~ 200,000 FWHM at m/z 400
Resolution
> 100,000 FWHM at m/z 1,000
664.3820
R=171040
z=2
787.4543
R=154169
z=1
1093.5867
R=125517
z=4
20
1500
2000
0
500
1000
m/z
1341.7392
R=106010
z=5
1620.8704
R=100972
z=3
1500
Analysis of histone H4 demonstrates the complementary nature of the HCD and ETD fragmentation techniques in the Orbitrap Elite instrument. Analysis using HCD and ETD in a single
run significantly improved sequence coverage over each of the techniques used separately.
2000
A total solution
for metabolism and metabolomics research
The Orbitrap Elite system offers a maximum resolution of >240,000 FWHM that allows complete visibility
into sample components and distinguishes them from complex matrix components. Improved sensitivity
ensures detection of more metabolites in biological samples. High-quality MSn spectral trees provide
extensive structure information for metabolite identification and location of modification sites. Improved
robustness enables true 24/7 operation.
Uncompromised performance
• Resolve isobaric species and isotope fine structures for isotope
tracing experiments
• Fast scan speed and high resolution improve precision and confidence
for quantitation experiments
•MSn de novo structural elucidation for identification of novel metabolites
on a UHPLC time scale
0.1 ppm
• Lower maintenance, and higher productivity when analyzing low-level
lipids and metabolites in complex biological matrices
Buspirone
MS Spectrum
R > 250,000
386.25510
R=251601
700000
Intensity
RT: 1.95 - 6.06
3.87
100
90
500000
400000
80
Relative Abundance
600000
300000
Buspirone
5 full MS scans
4 MS/MS scans
70
60
387.25830
R=252404
200000
100000
388.26111
R=213904
0
50
386.5
387.0
40
387.5
388.0
m/z
30
20
10
0
2.0
2.5
3.0
3.5
4.0
Time (min)
4.5
5.0
5.5
6.0
Even when acquiring MS and MS/MS data, the increased scan speed of the Orbitrap Elite system provides excellent coverage over narrow LC peaks. This analysis of buspirone
demonstrates acquisition of full-scan data at a quarter of a million resolution at one Hz. In total, nine scans were acquired during the elution of the peak: five full MS scans and
four MS/MS scans.
7
High-resolution, accurate-mass full-scan MS spectrum of an oxidative metabolite of buspirone
512.2684
1,800,000
Intensity [arbitrary units]
1,600,000
534.2498
1,400,000
150,000
0.50 ppm
100,000
402.2502
R=246701
50,000
1,200,000
0
1,000,000
400
800,000
494.2578
600,000
550.2151
464.2835
400,000
412.2851
200,000
0
200
300
400
500
600
700
800
900
1000
m/z
The Orbitrap Elite system provides accurate mass determination even in complex bile matrices. Here, an oxidative metabolite of buspirone
eluting in the window of several bile matrix components was still detected with an absolute mass deviation of 0.5 ppm.
Total solution for Metabolism and Metabolomics
Metabolism
Metabolomics
Thermo Scientific MetWorks and Thermo Scientific Mass Frontier software can
Thermo Scientific SIEVE and Mass Frontier software utilize the ultrahigh-
utilize the ultrahigh-resolution accurate-mass data from the Orbitrap Elite system
resolution accurate-mass data from the Orbitrap Elite for comprehensive analysis
for streamlined xenobiotic metabolite profiling and identification. The
MetWorks™
software integrates acquisition, processing and reporting for metabolite identifican
tion from LC/MS analyses and offers in-depth data mining tools for expected
and unexpected metabolites. The Mass
Frontier™
software provides chemically
n
of endogenous metabolites. The SIEVE™ software finds components that have
reproducible differences across multiple sample groups. Optimized component
detection dramatically decreases false positive identifications caused by chemical
background and redundant signals. The SIEVE software searches local and
intelligent annotation of MS spectral trees with automated fragment prediction.
web-based databases for putative identification. Mass Frontier software provides
Fragment Ion Search (FISh) in Mass Frontier software allows for automated
confirmation of known compounds through interpretation of MSn spectra, as well
localization of biotransformations.
as de novo structural elucidation for unknown compounds.
Application-specific software
turning data into information
Thermo Scientific Xcalibur Data System
Stable operating platform
Xcalibur™ software is the versatile, easy-to-use data system that
controls all Thermo Scientific MS systems. The home page of the
Xcalibur software offers easy navigation through the process of
instrument setup, sequence setup, and data acquisition.
Xtract
Xtract software deconvolutes isotopically resolved data for intact
protein analyses and complex MS/MS spectra acquired in top-down
analyses.
Thermo Scientific
Proteome Discoverer Software
Mass informatics platform for protein scientists
Proteome Discoverer™ software is a workflow-based proteomics
data processing software for in-depth data mining of complex
LC-MSn data sets. With the ability to exploit data from different
dissociation techniques (CID, HCD, ETD, IRMPD and ECD), Proteome
Discoverer software provides extra certainty for peptide and protein
identifications. Optional inclusion of multiple search algorithms
increases analytical flexibility, and results can now be merged into a
single report for easier interpretation.
Thermo Scientific ProSightPC Software
ProSightPC™ software was developed to address the specific
requirements of a top-down and middle-down proteomics strategy.
The fragmentation spectra of multiply charged, intact proteins are
exceedingly complicated. The software processes high-resolution,
accurate-mass data from the Orbitrap Elite MS to produce a list of
neutral fragment masses and performs comparisons to proteome
databases to identify and characterize proteins.
Thermo Scientific ProteinCenter Software
Revealing meaningful biological information
ProteinCenter™ software is a web-based data interpretation tool that
enables scientists to compare and interpret proteomic data sets and
extract meaningful biological information quickly and easily. It provides
access to a single database consolidated from more than 20 public
databases, including all historical data.
Thermo Scientific Pinpoint Software
Facilitating quantitative proteomics
Pinpoint™ software facilitates the transition from early-stage biomarker
discovery to larger-scale, quantitative verification of putative biomarkers and general quantitative proteomics. Pinpoint software simplifies the
creation of targeted quantitative assays. It allows researchers to leverage
previously acquired data from discovery experiments. Pinpoint software
largely automates the development of preliminary methods. It enables
acquisition and analysis of preliminary data, which is in turn used to
optimize the method.
SimGlycan™ Software
Predicting glycan and glycopeptide structure
SimGlycan software analyzes mass spectrometric data to predict the
structure of glycans and glycopeptides. It compares experimental MS/MS
data against its own database of theoretical fragments and generates a
list of probable candidate structures. Each candidate structure is scored
to reflect how closely it matches the experimental data. The software
also provides additional biological information for probable glycans and
identified glycopeptides.
Thermo Scientific MetWorks
Drug Metabolism Software
Simplify the interpretation of complex metabolism data
MetWorks software simultaneously searches multiple modifications of
one or more parent drugs and interprets simple to complex isotope
patterns, or unexpected or low-abundance metabolites.
Thermo Scientific Mass Frontier
Spectral Interpretation Software
Confident path from spectra to structure
Mass Frontier software allows confident structural elucidation through
chemically intelligent spectral annotation, state-of-the-art fragmentation
prediction, and unparalleled spectral and fragmentation mechanism
knowledge management.
Thermo Scientific SIEVE
Differential Expression Software
Analysis of differential expression based on comparison of LC-MS datasets
SIEVE software provides label-free, semi-quantitative differential analysis
of proteins, peptides, or metabolites from the comparison of multiple
LC-MS data sets. It is a statistically rigorous tool for analyzing data
from metabolism or biomarker discovery experiments.
www.thermoscientific.com/OrbitrapElite
BR30232_E 06/11G
©2011 Thermo Fisher Scientific Inc. All rights reserved. SimGlycan is a registered trademark of Premier Biosoft International Corp. Tandem Mass Tags is a registered trademark
of Proteome Sciences plc. Mass Frontier is a trademark of by HighChem Ltd.. ProSightPC is a trademark of the University of Illinois at Urbana-Champaign. All other trademarks
are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries.
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