Download CHO-TIM3 Cell Line

Datasheet
CHO-TIM3
Catalog#: YMAK-C011
Cell line ID: CHO-TIM3
Species: Cricetulus griseus
Cell Type: Chinese hamster ovary cell
Description: CHO cell line stably expressing exogenous human TIM3 gene.
Gene ID/Accession#: BC063431
Selection Marker: GS selection system
Application: TIM3 mAb screening
Morphology: epithelial-like,adherent
Medium: 302 Serum-free for CHO Cells
Subculture: Seed out at ca.1-1.5×106 cells/80 cm2. A subcultivation ratio of 1:4 to 1:8 is recommended.
Incubation: 37℃ With 5% CO2
Storage: Frozen in liquid nitrogen with 80% 302 medium, 10% FBS and 10% DMSO
Doubling Time: 20-24 hours
Biosafety level: 1
References: N/A
Background
Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted
to have an inhibiting function.
Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR
stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues.
Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In
progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.
Cell Line Generation
CHO-TIM3 Cell was generated using pMac-TIM3 vector expressing human TIM3 gene based on in house
CHO-GSKO cell line.
Characterization
上海市浦东新区蔡伦路 780 号 3 楼 3-G 室
Mali:[email protected]
http://cn.immuneonco.com
Tel:+86-21-58356573
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Datasheet
FACS analysis of cell surface TIM3 expression by an anti-human TIM3 antibody.
Cell Line Limited Use License
FOR IN VITRO REASEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE. BY ACCEPTING THIS
PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT.
This product may not be resold or transferred by the recipient and may be used only by the recipient, in their facility and only
for (1) research use, (2) drug discovery and development of biologic drugs, (3) quality assurance testing of biologic drugs,
and (4) assays for biologic drugs. No other commercial use is allowed. “Commercial” use means any and all uses of this
product by recipient for monetary or other consideration, including providing a service, supplying information or data to
unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically
engineer or otherwise create variations of the Cells.
上海市浦东新区蔡伦路 780 号 3 楼 3-G 室
Mali:[email protected]
http://cn.immuneonco.com
Tel:+86-21-58356573
2 of 2