Download (March – May 2016) Dates Trainings Contents No. of Days

Goal to Excite Confidence, Curiosity, Creativity and Perseverance
SCHEDULE ANNOUNCED (March – May 2016)
Dates
Trainings
25 March 2016
*DNA Isolation
Contents
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Strategies for DNA Isolation
Samples Preparation (2)
Reagents Preparation
DNA Extractions
DNA Quantification
Agarose Gel Electrophoresis (AGE)
Analysis of Bands
Troubleshooting
No.
Registration Fee (RS)
of
Days
Regular: 6000
1
Student UAF: 3600
Employee UAF: 4200
Other Govt. Institutions: 4800
26 March 2016
7-9 April 2016
*PCR
Gene Cloning
21 April 2016
*Protein Isolation
22-23 April 2016
*SDS-PAGE
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Overview of PCR
Introduction about Primer
Designing
Reaction Preparation
Thermocycler Instrumentation
PCR Running
Agarose Gel Electrophoresis (AGE)
Analysis of Bands
Troubleshooting
Basic Concepts of Recombinant
DNA Technology
Culture Preparations
Preparation of Competent Cells
Ligation of Gene into Vector
Transformation of Vector into Host
Cloning Strategies
Blue White Screening
Interpretation of Colonies
Plasmid Miniprep
Troubleshooting
Guide to Protein Extraction
Samples Preparation (2)
Reagents Preparation
Extraction of Proteins
Quantification of Proteins
Troubleshooting
Principle of SDS-PAGE
Reagents Preparation
Gel Castings
1
Regular: 6000
Student UAF: 3600
Employee UAF: 4200
Other Govt. Institutions: 4800
3
Regular: 15000
Student UAF: 9000
Employee UAF: 10500
Other Govt. Institutions: 12000
1
Regular: 6000
Student UAF: 3600
Employee UAF: 4200
Other Govt. Institutions: 4800
2
Regular: 10000
Student UAF: 6000
Employee UAF: 7000
Other Govt. Institutions: 8000
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6-7 May 2016
DNA
Fingerprinting
20 May 2016
*RNA Isolation
21 May 2016
*RT-PCR
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Sample Preparation, Loading and
Running
Staining of Gels
Protein bands analysis
Results Interpretation
Gel Preservation
Excision of Bands
Troubleshooting
Idea behind DNA Finger Printing
RAPD
DNA Extraction (2)
Polymerase Chain Reaction (PCR)
Agarose Gel Electrophoresis (AGE)
RAPD Analysis
Results Interpretation
Troubleshooting
Strategies for RNA Isolation
Samples Preparation (2)
Reagents Preparation
RNA Extraction
RNA Quantification
Agarose Gel Electrophoresis (AGE)
Analysis of Bands
Troubleshooting
Overview of RT-PCR
Primers of RT-PCR
cDNA Synthesis
Reaction Preparation
Thermocycler Instrumentation
RT-PCR Running
2
Regular: 7000
Student UAF: 4200
Employee UAF: 4900
Other Govt. Institutions: 5600
1
Regular: 6000
Student UAF: 3600
Employee UAF: 4200
Other Govt. Institutions: 4800
1
Regular: 6000
Student UAF: 3600
Employee UAF: 4200
Other Govt. Institutions: 4800
28 May 2016
Bioinformatics
(Basics)
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Agarose Gel Electrophoresis (AGE)
Results Interpretation
Troubleshooting
Introduction to Bioinformatics
Biological Databases
Sequence Analysis
Sequence Alignment
Primer Designing
Genomics
Proteomics
Phylogenetic Analysis
2
Regular: 6000
Student UAF: 3600
Employee UAF: 4200
Other Govt. Institutions: 4800
Note: 4-8 participants/ Training except Bioinformatics (Basics), 6-20 participants. If you are interested,
register immediately to confirm your spot.
For Registration: Download Proforma from www.molcare.org and send at [email protected]
*Special Offers are also available. Please contact Manager Trainings.
Nazia Kanwal
Manager Trainings
PhD scholar
Molecular Care
Department of Biochemistry, UAF
Email: [email protected]
Mob: 0333-1929859
For further details and disclaimers: www.molcare.org