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BioMEMS term project
Fully Automated Bio-Immobilization
System Using Microfluidic Channel and
Electroactive Substrate
T
HOM E
N E
WO R K
Contents
1. Introduction
2. Method
2.1 Fabrication of Au electrode patterns
2.2 Bio-immobilization
3. Results and Discussion
4. Conclusion
INTRODUCTION
Introduction
METHOD
RESULTS
CONCLUSION
What is Home network?
INTRODUCTION
Microarrayer
Photodeprotention
 Limitation : High cost instrument, Difficult quantification of the deprotection, Complex
treatment steps
INTRODUCTION
Introduction
METHOD
RESULTS
CONCLUSION
What is Home network?
INTRODUCTION
 Fully automated
microfluidic device
capture molecule immobilization
 High-throughput capture
molecule immobilization.
 Label free detection using
SPRi of EIS.
label free detection
RESULTS
INTRODUCTION
METHOD
Fabrication of Au electrode pattern
Mask Design
CONCLUSION
RESULTS
INTRODUCTION
CONCLUSION
METHOD
Fabrication of PDMS channel
Mask Design
Cover
PDMS replicas
Polymer Mold
Soft-Lithography
RESULTS
INTRODUCTION
METHOD
Overall Fabrication Process
CONCLUSION
RESULTS
INTRODUCTION
CONCLUSION
METHOD
Electroactive Capture Molecule Immobilization
RESULTS
INTRODUCTION
CONCLUSION
METHOD
Single Chamber Patterning & Detection
1st
+0.7 V
electrode
Injection
1st capture
molecule
SPRi
+0.7 V
2nd electrode
& 2nd molecule
injection
Automated
system
EIS
INTRODUCTION
CONCLUSION
METHOD
RESULTS
FEMM simulation
Apply electric field to electrode
Only voltage change specific
electrode which we want to
attach molecules
We will be able to bind molecules
INTRODUCTION
METHOD
CONCLUSION
RESULTS
Electrochemical Impedance Detection
INTRODUCTION
METHOD
CONCLUSION
RESULTS
SPR imaging
SPR
Merits
1)Kinetics of biomolecular interactions
can be measured in real time.
2)The adsorption of unlabled analyte
molecules to the surface can be
monitored.
3) SPR has high degree of surface
sensitivity that allows weakly bound
interactions to be monitored in the
presence of excess solution species.
INTRODUCTION
METHOD
CONCLUSION
RESULTS
SPR Imaging(SPRi) Detection
Line profiles of the captured molecule
difference images
a)The profiles show that the specific
molecules captured
b)Cross-reactivity was also significant
c)The change in background signal
was negligible due to the some
molecules resistance
d)Some background increase was
apparent on the amine-terminated
regions due to the physisorption of the
antibody
V.Kanda et al. Anal.Chem. 2004, 76, 7257
INTRODUCTION
METHOD
RESULTS
CONCLUSION
 Fully automated microfluidic device can be used for highthroughput capture molecule immobilization.
 Electroactive Self-assembled monolayer (SAM) provides
advantage of interference-free, simple and low-cost pattern
manufacturing.
 Capture molecule immobilization and target biomolecule
binding event can be monitored in real-time with SPRi
detection system.
 Electrochemical label-free detection system would make
this microfluidic device possible for the application of pointof-care (POC) and ubiquitous health (U-health) system.
The End
- Thank you -