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Increase your protein yield with Nmr-Bio rich labeling media for the production of [1H,13C]-Methyl, U-[D] proteins. The use of NMR-Bio rich labeling media is a cost effective solution to : • Enhance protein yield • Increase protein solubility • Speed-up protein production • Produce protein libraries in parallel • Simplify purification Cell growth in D2O using Production of a 37 kDa U-[D] protein 13CH -Alaβ, 3 Nmr-Bio medium 3 MW kDa M9 M9/D2O medium Ins. O.D. (600 nm) Due to the toxicity of D2O, the yield of many valuable protein targets, overexpressed in D2O/M9 media, is considerably reduced compared to standard H2O/LB based cultures. Furthermore, many interesting proteins are recovered in inclusion bodies when produced in perdeuterated M9 medium, requiring complex refolding steps. Sol. Nmr-Bio medium Ins. Sol. 116 2 Increase soluble proteins 66 45 1 35 25 100 300 500 Time (min) Reduce proteins in inclusion bodies An increase of protein yield by a factor of 3 to 10 has been reported by NMR-bio kits users. NMR-Bio–RLM rich labeling media are supplemented with perdeuterated 12C-carbon and 15N-nitogen sources and are calibrated for direct addition into pure M9 D2O culture medium (w/o glucose & ammonium chloride). The version which is nitrogen and carbon sources-free (NMR-Bio–RLC ) is also available to respond to your specific need! Please visit our booth for more information. Prices/volume of culture Examples of NMR-Bio 13CH 3 rich labeling media SLAM–RLC Rich labeling complement for single labeled methyl group (does not contain 15N and 12C sources) DLAM-RLC Rich labeling complement for double labeled methyl groups (does not contain 15N and 12C sources) TLAM–RLC Rich labeling complement for triple labeled methyl groups (does not contain 15N and 12C sources) groups Labeled Single methyl group (Aβ or Mε or Tγ) Two methyl groups combination (Aβ / Mε or Aβ / Tγ or Mε / Tγ ) Three methyl groups combination (Aβ / Mε / Tγ) SLAM–RLM Single methyl group Rich labeling medium for single labeled methyl group (including U-[2H] 15N and 12C sources) (Aβ or Mε or Tγ) DLAM-RLM Two methyl groups combination Rich labeling medium for double labeled methyl groups (including U-[2H] 15N and 12C sources) (Aβ / Mε or Aβ / Tγ or Mε/ Tγ ) TLAM–RLM Three methyl groups combination Rich labeling medium for triple labeled methyl groups (including U-[2H] 15N and 12C sources) (Aβ / Mε / Tγ) 0.25 L 0.5 L 1 L 390 € 590 € 990 € 520 € 790 € 1290 € 590 € 890 € 1500 € 650 € 990 € 1600 € 720 € 1090 € 1800 € 790 € 1190 € 2000 € * Prices in Euro (€) are converted into USD ($) and Yen (¥) according to the currency exchange rate corresponding to the order date. The listed prices exclude shipping fees and importation tax. Discounts apply for larger quantities. For specific quote contact us at [email protected]. Visit Nmr-Bio booth and get 10% discount on quotes issued during the conference ! More than 100 kits available online! [email protected] – RESEARCH USE ONLY – PRINTED IN FRANCE – 06/2015 –