Download Radiation induced changes in levels of selected proteins in

RADIATION INDUCED CHANGES IN LEVELS OF SELECTED PROTEINS
IN PERIPHERAL BLOOD SERUM OF BREAST CANCER PATIENTS AS A
POTENTIAL TRIAGE BIODOSIMETER FOR LARGE-SCALE
RADIOLOGICAL EMERGENCIES
Deperas-Kaminska Marta1,2, Bajinskis Ainars1,3, Marczyk Michal4, Polanska Joanna4,
Wersäll Peter5, LidbrinkElisabet5, AinsburyElizabeth A.6, Guipaud Oliver7,
Benderitter Marc7, Haghdoost Siamak1 and Wojcik Andrzej1
1: CRPR, Stockholm University, Sweden; 2: Institute of Mother and Child, Warszawa,
Poland; 3: Faculty of Medicine, University of Latvia; 4: Data Mining Group, Institute
of Automatic Control, Silesian University of Technology, Gliwice, Poland; 5: Division
of Radiotherapy, Radiumhemmet, Karolinska University Hospital, Stockholm,
Sweden; 6: Public Health England Centre for Radiation, Chemical and
Environmental Hazards, Chilton, Didcot, Oxford; 7: Institute for Radiological
Protection and Nuclear Safety, France.
Corresponding author’s email: [email protected]
The threat of a large scale radiological emergency, where thousands of people may
require fast biological dosimetry for the purpose of triage, make it necessary to search
for new, high throughput biological dosimeters. We tested an assay based on the
quantitative analysis of selected proteins in peripheral blood serum. We were
particularly interested in testing proteins that are specific to irradiation of skin, as
these can be used in cases of partial body exposure. Candidate proteins were
identified in an earlier study with mice, where skin of the animals was exposed to
different doses of radiation and global expression of serum proteins was analysed.
Eight proteins were found, the expression of which showed a consistent doseresponse relationship. Human analogues of these proteins were identified and their
expression was measured in peripheral blood serum of 16 breast cancer patients
undergoing external beam radiotherapy. The proteins were Apolipoprotein E;
Apolipoprotein H; Complement protein 7; Prothrombinase; Pantothenate Kinase 4;
Alpha-2-macroglobulin; Fetuin B and Alpha-1-Anti-Chymotrypsin. Measurements
were carried out in blood samples collected prior to exposure (control), on the day
after 1 fraction (2 Gy), on the day after 5 fractions (10 Gy), on the day after 10
fractions (20 Gy) and 1 month after 23-25 fractions (total dose of 46-50 Gy).
Multivariate analysis was carried out and a multinomial logistic regression model was
built. The results indicate that the combined analysis of Apolipoprotein E, Factor X
and Pantothenate Kinase 4 allows discriminating between exposure to 2 Gy and lower
and between 10 Gy and higher. The discrimination is possible up to one month after
exposure.
Funded by European Union's Seventh Framework Programme (FP7/2007-2013) under
grant agreement number 241536.