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Title︰Investigation of Functional Relationships between FIN219 and FHY1 Genes. M.S. Student ︰Tuan – Ya Chang(R92B42010) Advisor︰Dr. Hsu-Liang Hsieh Abstract : Phytochromes are plant photoreceptors that regulate plant growth and development in response to the light environment. In Arabidopsis, phytochrome A (phyA) is the primary photoreceptor responsible for perceiving and mediating various responses to far-red light. Previous studies have identified multiple phyA signaling components, such as FIN219 (far-red insensitive 219) and FHY1 (far-red elongated hypocoty 1). FHY1 encodes a product (FHY1) that specifically transduces signals downstream of the far-red (FR) light-responsive phytochrome A (PHYA) photoreceptor. FIN219 is an auxin-inducible gene and also involved in phytochrome A signaling pathways. Complementation test between fin219 and fhy1 show nonallelic noncomplementation, which indicates possible genetic interactions between these two mutations. Nonallelic noncomplementation is considered a hallmark for genes whose products act closely within the same pathway and most likely interact directly. Hence, we suggest that FHY1 may interact with FIN219 when response to far-red light. The results of yeast two-hybrid show that the C-terminus of FIN219 may interact with FHY1. When FIN219 overexpression construct was introduced into fhy1 mutant, the transgenic plant seedlings show shorter hypocotyls under far-red light condition, partially rescuing fhy1 phenotype. When FHY1 was overexpressed in fin219 mutant background, FHY1 can also partially rescue fin219 phenotype. In addition, it was found that FHY1 transcript levels increased in the fin219 mutant; in fhy1 mutant, FIN219 transcript levels increased as well.. Taken together, these data indicate that FHY1 and FIN219 coordinate with each other and response to far-red light to regulate photomorphogenesis.