Download 11 ·5·92 - Society for Natural Immunity

11 ·5·92
September-Oetober 1992
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S. Karger
Medical and Scientific
Publishers
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Paris· London
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Supplement Issue
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European Urology (ISSN 0302-28381, Vol. 21, Suppl. 1 (1992)
Editor: C.C. Schul man, Brussels
.Edito1r:. Franeo Di Silverio, Rome
Proceedings of
the 2nd Mediterranean
Congress of Urology
arkers in
Urology
Rome, July 3-6,199
An extensive survey of the
diagnostic and prognostic
roles of tumor markers.
Beta-Human Chorionic Gonadotropin and AIpha-Fetoprotein in Central and Peripheral Venous Blood of Patients with Testicular Tumors
Kidney Tumors
Contents
Bladder Tumors
Javadpour, N.,' Guirguis, R: Tumor Collage-
nase-Stimulating Factor and Tumor Autocrine Motility Factor as Tumor Markers in
Bladder Cancer· An Update· Sanchez-Fernandez de Sevilla, C.; MorelI, L.; Gi/-Salam,
M. et a/.: Morphometric and Immunohistochemical Characterization of Bladder Carcinoma in situ and Its Preneoplastic Lesions .
Schmidt, A.;Bub, P.;Rüther, U. etal.:Tissue
Polypeptide Antigen for Monitoring of Ad-
vanced Bladder Cancer after MVEC Chemotherapy . Romero, J.; Ales, L.; Mal/offe, C. et
a/: BlooderWash Cytology and Flow Cytometryfor the Diagnosis of Transitional Cell Carcinoma ofthe Urinary Bladder . Sanchez-Fernandez de Sevilla, M. c.; Gil-Salom, M.; PerezBacete, M. et al: DNA-Ploidy, MorphometricStereological and P-Glycoprotein Study of
Superficiaf Bladder Carcinomas . Di Silverio,
F.; von He/and, M.; Oe Berardinis, E. et a/:
~r!?gnostjc Role of Flow Cytometry in Superflclal BladderCancer· Saracino, G.A.;Ditonno, P.; Disabato, G. et al.: Prediction of Recurrence and Progression in Primary Superficial
Bladder Cancer with DNA Flow Cytometry .
Marandofa, P.; LardennOiS, B.; Pfoton, D. et
af: A New MarkerforEarly Detection and Indicator of Progression of Cancer of the Bladder.
Preliminary Results with Ag-NOR Index in 38
Cases of Superficial Bladder Cancer
Testicular Tumors
Javadpour, N.: Current Status of Tumor
Markers in Testicular Cancer. A Practical Review . Zenico, T.; Zoli, M.; Fiori, M. et al.:
Eighth International
Natural Killer Cell Workshop and
First Meeting of the
Society for Naturallmmunity
Uzard, G.; Roignot, P.; Dusserre-Guion, L er
a/.:Characterization of Seven Kidney Tumors
by Flow Cytometry: Analysis of Cell Cycle,
DNA Content and P-Glycoprotein Expression
. DiSilverio, F.;Gaffucci, M;Flammia, G.P. et
al.:Biological and Clinicallmplication ofCellular DNA Content in Renal Cell Carcinomas .
Corrado, F.; Mannini, 0.; Ferri, C. et a/.: The
Prognostic Significance of DNA Ploidy Pattern in Transitional Cell Cancer of the Renal
Pelvis and Ureter: Contil).uing Follow-Up
Dönmez, T.; Ka/e, M; Ozyürek, Y. et al.:
Erythrocyte Sedimentation Rates in Patients
with Renal Cell Carcinoma . Trombetta, C.;
Berretta, A.; Siracusano, S. et a/: Evaluation
of Hematochemical Parameters and Renal
Echography after ESWL
Veneziano, L;
D'Angefo, A.R.;Burrai, L eta!: DNA Markers
in Diagnosis of Adult Dominant Polycystic
Kidney Disease
Prostatic Carcinoma
Schalken, J.A.; Debruyne, F.MJ.: Identification of New Prostate Cancer Progression
Markers by Differential Hybridization Analysis de Matteis, A.: Tissue Markers in the
Diagnosis and Prognosis of Prostatic Carcinoma Marandofa, P.; Lardennois, 8.; Ploton, D. et al: Nucleolar Organizer Regions:
Preliminary Results of the Clinical Use of a
New Marker for Prostatic Carcinoma (40
Cases) . Cfar-Blanch, F.; Morell-Quadreny,
L; Fenoffosa-Entrena, B. et a/.: Correlation
between Serum Values of Prostatic Acid
Phosphatase and Morphometric Analysis in
the Cytologic Diagnosis of Prostatic Carcinoma . Di Si/verio, F.; D'Eramo, G.; Flammia,
GP. et al.: New Ultrasensitive Assay Development by Using Monoclonal Antibodies for
Detecting Prostate-Specific Antigen . Romics, f.; Ga/amb, L.: Changes in Prostate-Specific Antigen and Prostatic Acid Phosphatase
Concentration following Prostatic Examination in Benign Prostatic Hypertrophy and
Prostate Cancer Patients . Puppo, P.; Perachino, M; Ricciotti, G. et a/.: Comparison
between Digital Rectal Examination, Prostate-Speciflc Antigen and Transrectal Ultrasound in Symptomatic Patients. Results on
141 Cases . Di Silverio, F.; D'Eramo, G.;
Caponera, Met al.:The Prognostic Value of
DNA Content in Patients with Prostatic Carcinoma . Zanetti, G.; Trinchieri, A.; Dei Nero,
A. et al: Prognostic Significance of ProstateSpecific Antigen in Endocrine Treatment for
Prostatic Carcinoma . Zatton;, F.; Piazza, R.;
V~anello, R. ~taf.:Preoperativeand Postoperative Evaluation of Prostate-Specific Antigen
i~ Localized Prostatic Cancer Treated by Radlcal Pros!atectomy . Marcelfini, M.; DeCarli,
P.;Abbollto,MR. et al.:Serum Osteocalcin in
Monitoring Bone Metastases in Advanced
Prostatic Cancer . Curatofo, c.; Ludovico,
G.M; Correa!e, M et al.: Advanced Prostate
Cancer Follow-Up with Prostate-Specific Antigen, Prostatic Acid Phosphatase, Osteocalcin and Bone Isoenzyme of Alkaline Phosphatase
.....................................
I
I
I
I
Molecular and Cellular
Aspects of Natural Killer
Cell T riggering and
Signalling
October 4-6, 1992
St. Petersburg Beach,
FL,USA
I
Benign Prostatic Hypertrophy
Omacini, S.; Mione, R.; Barichelfo, M et al.:
Relationship between Prostatic Acid Phosphatase and Prostate-Specific Antigen Serum Levels and Prostatic Volume in Benign
Prostate Hyperplasia. Pitfall on Tumor Markers Assessrnent in Primary Prostatic Cancer?
. ~im~ek, U.; Kutlu, S.; Yava.~9aoglu I. et al.:
Seasonal Variation of Prostatic Acid Phosphate and Prostate-Specific Antigen in Patients without Prostatic Malignancy
Fields oi Interest
Urology,Oncology
Markers in Urology
Proceedings ofthe 2nd Mediterranean Congress of Urology
Rome, July 3-6, 1991
'European Urology', Vol. 21, Suppl. 1 (1992)
Editor: Di Silverio, F. (Rome)
IV + 116 p., 62 fig., 54 tab., soft cover, 1992
SFe. 42.- f DM 50.- f f 18.30 f US$ 33.75
Prices subject to change
US $ price for USA, f price for UK, DM price for FRG only
ISBN 3-8055-5612-8
KARGER
KAR.GER.
Abstract Editors
Eva Lotzova,
Hauston, TX, USA
Julie Y Djeu,
Tampa, FL, USA
John C Ruckdeschel,
Tampa, FL, USA
Q
Contents
Eighth International Natural Killer Cell Workshop and
First Meeting of the Society for Naturallmmunity
..............................................................................
Generously supported by
.. .................. .. .................................................................................................
H. Lee Moffitt Cancer Center & Research Institute
U niversity of South Florida
••••••••••.•••••••••• ..................................................................................................
Session I
Abstracts
Genetics Institute
Hoffmann-La-Roche Inc.
Abstracts No.
1-34
(Invited Speakers: 1-5)
Sandoz Corporation
...............
NK Cells in Infectious Disease and Cancer
241
.......................................................................................................
.
Session 11
~~------------------------------~258
35-63
(lnvited Speakers: 35-40)
NK-Related Genes: Expression and Function
·········································S~~~i·~~·III·
64-76
(Invited Speakers: 64-66)
..............................................................
NK Cells and Integrin/Adhesion Molecules
273
............................................................................................................ :........ ..
Session IV
.....................................
S. Karger
Medical and Scientific Publishers
Basel· Freiburg . Paris. London
NewYork· New Delhi· Bangkok
Singapore . Tokyo . Sydney
.................................... . .................................... .
Drug Dosage
The authors and the publisher have exerted
every cffon. to enSUTe that drug selection and
dosage set forth in this text are in aeeord with
eurrent recommeudations and praetiee at the
time of publicatioll. However, in view of ongoing research, changes in government regulations, and thc eonstant fiow of info=ation
relating to drug therapy and drug reaction5, the
reader i5 urged to check the paekage insert for
eaeh drug for any change in indications and
dosage and for added warnings and precautiOllS. This is partieularly impOitant when the
recommended agent is a new and/or infrequently employed drug.
All rights reserved.
No part of this publieation may be translated
iuto other languages, reproduced or utiJized in
any fonn or by any means, electronic or mechanieal, inc1uding photocopying, recording,
microcopying, or by any infonnation storage
and retrieval system, without pennission in
writing from the publisher or, in the case of
phOlocopying, direet payment of a speeified fee
to the Copyright Clcaranee Center (sec 'Infor_
mation for Readers and Subscribers').
© Copyright 1992 by S. Karger AG,
P.O. Box, CH-4009 Basel (Switlerland)
Printed in Switzerland on acid-free paper by
Thür AG Offsetdruck, Pratteln
ISBN 3-8055-5690-X
77-107
(Invited Speakers: 77-80)
NK Cells and Signal Transduction
279
.......................................................................................................................
Session V
108-138
(Invited Speakers: 108-110)
NK Cells and Cytokines
'" ................................................................................................................
295
~';3
Author Index
238
VIIIth Tut. NK Cell Workshop
239
Q
Contents
Eighth International Natural Killer Cell Workshop and
First Meeting of the Society for Naturallmmunity
..............................................................................
Generously supported by
.. .................. .. .................................................................................................
H. Lee Moffitt Cancer Center & Research Institute
U niversity of South Florida
••••••••••.•••••••••• ..................................................................................................
Session I
Abstracts
Genetics Institute
Hoffmann-La-Roche Inc.
Abstracts No.
1-34
(Invited Speakers: 1-5)
Sandoz Corporation
...............
NK Cells in Infectious Disease and Cancer
241
.......................................................................................................
.
Session 11
~~------------------------------~258
35-63
(lnvited Speakers: 35-40)
NK-Related Genes: Expression and Function
·········································S~~~i·~~·III·
64-76
(Invited Speakers: 64-66)
..............................................................
NK Cells and Integrin/Adhesion Molecules
273
............................................................................................................ :........ ..
Session IV
.....................................
S. Karger
Medical and Scientific Publishers
Basel· Freiburg . Paris. London
NewYork· New Delhi· Bangkok
Singapore . Tokyo . Sydney
.................................... . .................................... .
Drug Dosage
The authors and the publisher have exerted
every cffon. to enSUTe that drug selection and
dosage set forth in this text are in aeeord with
eurrent recommeudations and praetiee at the
time of publicatioll. However, in view of ongoing research, changes in government regulations, and thc eonstant fiow of info=ation
relating to drug therapy and drug reaction5, the
reader i5 urged to check the paekage insert for
eaeh drug for any change in indications and
dosage and for added warnings and precautiOllS. This is partieularly impOitant when the
recommended agent is a new and/or infrequently employed drug.
All rights reserved.
No part of this publieation may be translated
iuto other languages, reproduced or utiJized in
any fonn or by any means, electronic or mechanieal, inc1uding photocopying, recording,
microcopying, or by any infonnation storage
and retrieval system, without pennission in
writing from the publisher or, in the case of
phOlocopying, direet payment of a speeified fee
to the Copyright Clcaranee Center (sec 'Infor_
mation for Readers and Subscribers').
© Copyright 1992 by S. Karger AG,
P.O. Box, CH-4009 Basel (Switlerland)
Printed in Switzerland on acid-free paper by
Thür AG Offsetdruck, Pratteln
ISBN 3-8055-5690-X
77-107
(Invited Speakers: 77-80)
NK Cells and Signal Transduction
279
.......................................................................................................................
Session V
108-138
(Invited Speakers: 108-110)
NK Cells and Cytokines
'" ................................................................................................................
295
~';3
Author Index
238
VIIIth Tut. NK Cell Workshop
239
Abstracts
.....................................
Symposium Chairpersons
Julie Y. Djeu,
Tampa, FL, USA
lohn C. Ruckdeschel,
Tampa, FL, USA
i~~·i~~ds~~~k~~~~~d·ci.~i;~~;~~~~······················ ......... .
Session I
Paolo Allavena, MD
Hugh F. Pross, MD, PhD
Milan, ltaIy
Paul l. Anderson, MD, PhD
Boston, MA, USA
D. Kay Blanchard, PhD
Tampa, FL, USA
Ronald B. Herberman, MD
Benjamin Bonavida, PhD
Los Angeles, CA, USA
Sidney Golub, PhD
1erome Ritz, MD
Rika Ishikawa, Helen C Su, Christine A. Biron
Boston, MA, USA
Division of Biology and Medicine, Brown
University, Providence, R.I., USA
John C. Ruckdeschel, MD
Rome, Italy
Julie Y. Djeu, PhD
Tampa, FL, USA
Los Angeles, CA, USA
Perugia, Italy
Angela Santoni, PhD
Ocnver, CO, USA
Prcsident
1
Regulation of Splenie Natural Killer Cell
Responses During Virallnfections
Tampa, FL, USA
lames L. Cook, MD
Eva Lotzova, PhD
.................
David Raulet, PhD
Carlo Riccardi, MD, PhD
Providence, RI, USA
International Society for
Naturallmmunity
Kingston, Ont., Canada
Berkeley, CA, USA
Christine Biron, PhD
Öffi~~~~~f·ih~··················
.. ....................................................... .
William E. Seaman, MD
San Francisco, CA, USA
Paul M. Sondel, MD. PhD
Vice President
Ronald B. Herberman MD
Tuomo Timonen, MD
Pittsburgh, PA, USA
Secretary
leffrey P. Houchins, PhO
'
Minneapolis, MI, USA
Klas Karre, MD, PhD
Stockholm, Sweden
Ralf Kiessling, MD, PhD
Stockholm, Sweden
Eva Klein, MD, PhD
StockhoIm, Sweden
Vinay Kumar, MD
Madison, WI, USA
Walter Storkus, PhD
Durharn, N C, USA
Tuomo Timonen, MD
Helsinki, Finland
Giorgio Trinchieri, MD
Philadelphia, PA, USA
Rayrnond M. Welsh, PhD
Worchester, MA, USA
Stanley Wolf, PhD
Cambridge, MA, USA
DaUas, TX, USA
Lewis L. Lanier, PhD
I
.1
I
PaIo Alto, CA, USA
Paul l. Leibson, MD, PhD
Rochester, MN, USA
Eva Lotzova, PhO
Houston, TX, USA
Albert Mantovani, MD
Milan, Italy
Lorenzo Moretta, MD, PhD
Genova, Italy
John R. Ortaldo, PhD
Frederick, MD, USA
Eckhard R. Podack, MD, PhD
Miami, FL, USA
Natural killer (NK) cell activation and pro1ifera~
tion peak on day 3 and subside by day 5-7 post-infection of mice with either lymphocytic choriomeningitis
virus (LCMV) or murine cytomegaloviurs (MCMV).
Our laboratory has been characterizing the positive
and negative regulation of these NK ceIl responses.
Cytokine studies have demonstrated that interferons
(IFN) are potent positive regulators whereas transforrning growth factors-ß (TGF-ß) are potent negative
regulators of tbe in vivo NK cell responses. Tbe work
presented hefe was undertaken to evaluate cellular
interactions contributing to in vivo NK cell regulation.
Histological examination of seetions isolated from
LCMV-infected mice revealed dramatic changes in
splenie architecture at firnes coinciding with IFN production and NK cell activation and proliferation;
white pulp areas were increased but leukocyte concentrations in red pulp areas were decreased. To evaluate
IFN expression and tbe contribution of IFN to the
induction of the observed morphological changes,
C57BLl6 mice were treated with the chemical inducer
ofIFN, poly-inosinic-cytidylic cid (poly I:C). As early
as 3 h after poly I:C administration, biologically active
IFN was present in spleen and serum. Neutralization
studies and Northern blot analyses demonstrated that
IFN-ß was preferentially induced. Histological examination revealed poly I:C-induced splenic changes com~
parable to those observed during infection, i.e. dramatic increases in splenic white pulp area and de~
creases in red pulp leukocytes. The changes were
observed at 6-36 h post-treatment. Spleen weights and
splenic leukocyte yields remained relatively constant.
Cell transfer experiments with Dil~labeled ceHs dem~
onstrated that poly I:C enhanced the accumulation of
ceHs migrating from blood to white pulp regions. The
changes in splenie leukocyte distribution were shown
to be a result of IFN induction as: (1) treatment with
anti-IFN antibodies inhibited the poly I:C~induced
changes, and (2) administration of purified IFN~ß
induced similar changes. In situ hybridization and
immunohistochernical staining demonstrated that, at
early times post~treatment, cells expressing high levels
of IFN~ß were found in vasculature and dispersed in
red pulp regions. By later times post~treatment, the
intensely positive ceHs were localized deep within
white pulp regions and in perivascular spaces. The
results indicate that IFN induces a profound migration
ofleukocytes into white pulp regions ofthe spleen. The
data also suggest that a rninor sub~population ofleuko~
cytes is an especially potent and early producer ofIFN~
ß. Studies are underway to characterize the spienic dis~
tribution ofNK cells during the IFN-induced redistri~
bution and to determine how the architectural changes
may contribute to the regulation ofNK cell responses
during viral infections.
2
Natural Killer CelljNeutrophil
Interactions for Resistance against
Opportunistic Fungi
Julie Y. Djeu, Sheng Wei, Carla Palma, ~
Antonio Cassone, D. Kay Blanchard
Department of Medical Microbiology and
Immunology, H. Lee Moffitt Cancer Center and
Research Institute, University of South Florida
College ofMedicine, Tampa, Fla., USA
Candida albicans is an opportunistic fungal patho~
gen that causes persistent infection in immunocomprised hosts. To define if normal resistance might be
controlled by natural killer cells in concert with neutro~
phils, we isolated large granular lymphocytes (LGL)
from human peripheral blood by Percon gradients and
neutrophils after separation from mononuclear cells
with Ficoll-Hypaque gradients. Human LGL were
found not to be able 10 inhibit Candida growth in a
240
VIIIth Int. NK Cell Workshop
241
NatImmun
ISSN [0[8-89[6
NAIMEL
[[(5) 237-3[6 (1992)
Naturatyl
•mmunl
Eighth International Natural Killer Cell
Workshop and First Meeting of
the Society for Naturallmmunity
MOlecular and Cellular Aspecls 01
Natural Killer Cell Triggering and Signalling
...........
...........
...........
...........
...........
...........
...........
...........
...........
Abstracts
241
Author Index
313
11