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Transcript
BIO101 Homework DNA replication
1.
2.
3.
4.
5.
6.
name ________________
Below is a diagram of DNA replication in E. coli.
Which end (5’ or 3’) of the DNA molecule is here?
Which enzyme functions here to deal with supercoils in DNA?
What enzyme functions here to unwind the DNA?
Which enzyme functions to synthesize these small RNA sequences?
What are these ~1000 nucleotide long DNA fragments called?
Is this strand the leading or lagging strand
2
3
4
5
6
1
7. Refer to the figure above. Explain how this figure represents semiconservative replication.
8. In analyzing the number of different bases in a DNA sample, which 2 results are consistent with basepairing rules? (circle 2)
A. A = G
C.
A=C
E.
A +T = G + T
B. A+G = C + T
D.
G=T
F.
A/T = 1
9. What do the letters DNA stand for? _________________________________________
10. The nucleotide bases are paired by ____ bonds along the axis of the DNA molecule.
11. The DNA “backbone” is composed of _________ and __________ molecules
12. A cell has an adenine content of 17%. What is the % of the nucleotide, cytosine? __________
13. Write the complementary sequence to following DNA strand:
AATTCGCCGGTATTAGACGTT