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Download MEXi – iBAs´ MAMMAliAn EXprEssion systEM
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iba Solutions For Life Sciences Product information MEXi – IBAs´ mammalian expression system – High expression rates with perfectly matched HEK293E cell line, culture medium and expression vectors High protein yields Easy handling Optimized components Cost-effective Econom ic system for high ly p u re p ro teins Strep-tag www.iba-lifesciences.com Product information The MEXi system combines high protein yields with high purity Secreted alkaline phosphatase (SEAP) and a mAb, both fused with Twin-Streptag (TST), were expressed with the MEXi system and purified via the TST resulting in high yields of pure protein: 143 mg/l cell culture for SEAP and 96 mg/l for the mAb, heavy (HC) and light chain (LC). pA Integration sites Co lE ri Io Am pR pDSG-IBA or iP pDSG-IBA expression vector: Available with different tags, like Twin-Strep-tag, Strep-tag®II and 6xHis-tag and BM40 secretion signal. Protein expression in mammalian cells has experienced an increased usage within the last years. In contrast to other expression systems (like E. coli) recombinant proteins produced in mammalian expression systems can provide more post-translational modifications, such as glycosylation, which are a necessity for the functionality of the expressed proteins. IBA offers all essential components for efficient protein expression in mammalian cells with the mammalian expression system MEXi: Cat. No. 5-52XX-000 2-6010-010 Name pDSG-IBA Expression vectors MEXi-CM Culture Medium 2-6011-010 MEXi-TM Transfection Medium 2-6001-001 MEXi-293E HEK293E cell line www.iba-lifesciences.com HEK293E cell-line, cell-culture media and expression vector are perfectly matched to provide high protein yields without further optimization Transient expression for time and cost efficient protein production Efficient nuclear import and extrachromosomal replication due to vector encoded EBV origin of replication (oriP) and EBNA-1 provided by MEXi-293E cells Multiple expression vectors with different features available Twin-Strep-tag for highly pure proteins More in www.iba formation: -lifescien ces.com Version PI85-01-0001 V CM IBA GmbH Rudolf-Wissell-Str. 28 · 37079 Goettingen · Germany · Phone +49 551 50672-0 · Fax +49 551 50672-181 · [email protected] IBA US Distribution Center 1328 Ashby Road · Olivette · MO 63132 · USA · Phone 1-877-IBA-GmbH (1-877-422-4624) · Fax 1-888-531-6813 · [email protected]
