Download Effect of Neonatal Iron Supplementation on Microglial Activation in

Microglial morphology: what we
can learn about neuroinflammation
and Huntington’s Disease
Marley Realing
Microglia: Cause or Response to
Neuroinflammation?
•
Microglial are resident brain immune
cells
•
Activation state can be studied using
morphology
•
They both induce and react to
neruroinflammation
•
It has been shown there is a significant
amount of microglia activation in HD
and other neurodegenerative disease
•
The role of microglial activation in
neurodegeneration is unclear
IBA-1 labels Brain Microglial
A-B: anti-IBA1 antibody (Wako
Chemical)
A) 1:500 anti-IBA1
B) No primary control
C-D: anti-IBA1 antibody (Santa
Cruz)
C) 1:500 anti-IBA1
D) anti-IBA1 + blocking peptide
Four Microglia Morphologies were
Identified
A. Ramified: small cell body,
ramified processes
B. Primed: larger, more irregularly
shaped cell body
C. Reactive: large cell body and
retracted processes
D. Ameboid: enlarged cell body
and few, if any processes
Scale bars = 20 mm
Huntington’s Disease and T. gondii Change
Microglial Morphology in the Cortex
Wild-type
Huntington’s disease
C
Wild Type infected
with Toxoplasma
gondii
Scale bars = 20 mm
Conclusions and Future Directions
• Microglia
morphology differed in mice with HD compared to
control wild-type mice
• Wild-type
mice with Toxoplasma gondii infection due increases
in number, staining intensity, and morphological changes
• Future
plans:
• Studying
microglial morphology after HD-potentiating iron
treatment
• Quantitatively
software
differentiate morphology using Neuroleucida
Acknowledgements
Thank you to:
•
Wyoming INBRE fellowship for the Spring and Summer of 2017
•
Advisor: Dr. Jonathan Fox and David Donley
•
Lab Members: Sonal Agrawal, Julia Fox, Ashton Abarr, Gabrielle