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Hellenic Accreditation System S.A.
Annex F1/10 to the Certificate No. 213-4
SCOPE of ACCREDITATION
of the
Central Public Health Laboratory
of the
KEELPNO
of the
Ministry of Health
Materials/ Products tested
Types of test/ Properties measured
Applied standards/
Techniques used
Microbiological Tests
1.Enumeration of culturable microorganisms –colony count by inoculation in a
nutrient agar culture medium at 222 0C
and at 362 0C
ΕLΟΤ ΕΝ ISO 6222:2000
2.Detection and enumeration of
Pseudomonas aeruginosa
ELOT ΕΝ ISO 16266:2009
2.Water for human consumption,
natural mineral water, surface
waters intended for human
consumption
1.Detection and enumeration of
Clostridium perfringens (spores included)
Annex III of the J.M.D.
Υ2/2600/2001 as modified,
completed and valid until
now
3.Water for human consumption,
natural mineral water, surface
waters, sea water, pool water and
recreational waters
1.Detection and enumeration of
Escherichia coli and coliform bacteria
ΕLΟΤ ΕΝ ISO 9308-1: 2001
2.Detection and enumeration of intestinal
enterococci
ΕLΟΤ ΕΝ ISO 7899-2: 2001
4.Water for human consumption,
pool water, recreational waters
and other types of water
1.Detection and enumeration of Legionella
spp.
ISO 11731:1998
2.Detection and enumeration of Legionella
spp. – Part 2: Direct membrane filtration
method for waters with low bacterial counts
ISO 11731-2:2004
1.Water for human consumption,
natural mineral water, surface
water, pool water and recreational
waters
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Annex F1/10 to the Certificate No. 213-4
06.06.2013
Materials/ Products tested
5.Food
6.Clinical and environmental
Salmonella spp strains
7.Clinical and environmental
strains of other
Enterobacteriaceae
Types of test/ Properties measured
Applied standards/
Techniques used
1.Detection of Salmonella spp.
ΕLΟΤ EN ISO 6579:2003
2.Detection of Listeria monocytogenes
ΕLΟΤ ΕΝ ISO 11290.01:
1997 /Amd1:2004
3.Enumeration of Listeria monocytogenes
ΕLΟΤ ΕΝ ISO 11290.02:
1999 /Amd1:2004
4.Detection of E. coli Ο157:Η7
ΕLΟΤ ΕΝ ISO 16654:2001
5.Enumeration of coagulase-positive
Staphylococci (Staphylococcus aureus and
other species)
ΕLΟΤ ΕΝ ISO 6888-1:1999
/Αmd1:2004
1.Serotyping of Salmonella spp.
Slide Agglutination, White Kauffmann - Le Minor
Scheme_2007, 9th edition
2.Antimicrobial resistance of Salmonella
spp. in : Ampicillin, Cefotaxime,
Amoxicillin + clavulanic acid, Ceftazidime,
Chloramphenicol, Tetracycline,
Trimethoprim, Nalidixic, Amikacin,
Ciprofloxacin, Streptomycin, Kanamycin,
Netilmicin, Tobramycin
Antimicrobial Disk
Susceptibility Tests
CLSI:Μ2-Α10, Vol. 29,
No. 1
CLSI:M100- S21, Vol.31
No. 1
1.Antimicrobial resistance of
Enterobacteriaceae in: Ampicillin,
Amoxicillin +clavulanic acid, Piperacillin,
Piperacillin + tazobactam,
Ticarcillin,Ticarcillin + clavulanic acid,
Cefoxitin, Cephalothin, Cefotaxime,
Ceftazidime, Cefepime, Aztreonam,
Imipenem, Meropenem, Ertapenem,
Doripenem, Tetracycline,
Sulphamethoxazole + Trimethoprim,
Amikacin, Gentamicin, Ciprofloxacin
Antimicrobial Disk
Susceptibility Tests
CLSI:Μ2- Α10, Vol. 29,
No. 1
CLSI:M100- S21, Vol.31
No. 1
Chemical Τests
1.Water for human consumption,
natural mineral water, surface
waters and pool water
2.Water for human consumption,
natural mineral water, surface
waters and wastewater
Page 2 of 3
1. Determination of total hardness
APHA* 2340 C
2. Determination of calcium
APHA* 3500 - Ca B
3. Determination of magnesium
APHA* 3500 - Mg B
4. Determination of chloride ions
APHA* 4500 - Cl- B
5. Determination of alkalinity
APHA* 2320 B
6. Determination of Total Dissolved Solids
(TDS) dried at 180 °C
APHA* 2540 C
1. Determination of Total Suspended Solids
(TSS) dried at 103-105 °C
APHA* 2540-D
2. Determination of pH
APHA* 4500-H+
3. Determination of conductivity
APHA* 2510-B
Annex F1/10 to the Certificate No. 213-4
06.06.2013
Materials/ Products tested
3.Wastewater
Types of test/ Properties measured
Applied standards/
Techniques used
1.Determination of Biochemical Oxygen
Demand BOD 5 days
In house Method (ΜΔ-111),
based on APHA* 5210 D
2.Determination of Chemical Oxygen
Demand COD
In house Method (ΜΔ-110)
based on APHA* 5220 D
Molecular Methods
1.Clinical and environmental
strains of Enterobacteriaceae
2.Clinical and environmental
strains of Ε.coli (VTEC)
1. Bacterial typing
In house method (ΜΔ-26) for
the preparation, restriction
enzyme digestion and PulsedField Gel Electrophoresis of
agarose plugs
2. Detection of resistance genes blaVIM &
blaKPC
In house Method (ΜΔ-25)
1. Detection of toxin genes (vtx1 and vtx2)
Polymerase Chain Reaction
(PCR), based on the proposed
protocol of WHO
Collaborating Centre for
Reference and Research on E.
coli –Statens Serum Institute,
EU Reference Laboratory
VTEC Instituto Superiore di
Sanita
Polymerase Chain Reaction
(PCR)
* ΑPHA: American Public Health Association, American Water Works Association, Water Environment Federation, “Standard
Methods for the Examination of Water and Wastewater”, 21st Edition, 2005
Site of assessement: Permanent Laboratory premises, 34 Fleming str., 16672, Vari, Attiki, Greece.
Approved signatories: A. Vatopoulos, Em. Velonakis, A. Chaldoupi, G. Papageorgiou, I. Spiliopoulou, E.
Mathioudaki, T. Karabinas, M. Kontarini, A. Flountzi, M. Lambiri, G. Mandilara, P. Giakkoupi, K.
Trifinopoulou.
This Scope of Accreditation replaces the previous one dated 21.02.2013.
The Accreditation Certificate No. 213-4, to ELOT EN ISO/IEC 17025:2005, has been extended up to 21.09.2013.
Athens, June 06, 2013
Ioannis Sitaras
Director of the Laboratories Accreditation Division
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Annex F1/10 to the Certificate No. 213-4
06.06.2013