Download SEQUENCE ANALYSIS OF GENE CODING FOR PHENOL

SEQUENCE ANALYSIS OF GENE CODING FOR PHENOL HYDROXYLASE IN
ASPERGILLUS AWAMORI STRAIN
H. Yemendzhiev*, J. Manasiev, Z. Alexieva
Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bontchev Str., bl. 26, 1113Sofia, Bulgaria
Some members of Aspergillus genera are reported to exert a significant capacity in aromatic
compounds catabolism. In the present study the panfungal primer pair was used in PCR procedure with the
purpose to approve the taxonomic affiliation of a strain Aspergillus awamori NRRL 3112 by 18 S rDNA
sequence analysis. The investigated strain is able to degrade various aromatic compounds.
The first key enzyme of aromatics degradation is phenol hydroxylase (EC 1.14.13.7) which catalyzes
the conversion of phenols to their o-diol derivatives by incorporation of a single hydroxyl group into the
substrate. The aim of the present work was to identify and sequenced the gene encoding the phenol hydroxylase
in a strain of Aspergillus awamori. A three pairs of oligonucleotide primers were created on the bases of
sequences for proteins with phenol hydroxylase activities registered in NCBI. A 2071 bp DNA sequence was
obtained as a result of carried out PCR and sequence analyses. The established nucleotide and related protein
sequences were analyzed and compared with that of other enzymes with similar properties. All sequences
obtained in this investigation have been deposited in the NCBI nucleotide sequence databases under accession
numbers GQ472777, GQ279378, and ACT52897.
Key words: Aspergillus awamori; phenol hydroxylase; PCR; sequence analyses