Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download or rabbit anti-CD36 and mouse
Document related concepts
Blood transfusion wikipedia , lookup
Schmerber v. California wikipedia , lookup
Hemolytic-uremic syndrome wikipedia , lookup
Blood donation wikipedia , lookup
Autotransfusion wikipedia , lookup
Hemorheology wikipedia , lookup
Men who have sex with men blood donor controversy wikipedia , lookup
Jehovah's Witnesses and blood transfusions wikipedia , lookup
Transcript
CD36 associates with CD9 and integrins on human blood platelets by Wei-Min Miao, Eliza Vasile, William S. Lane, and Jack Lawler Blood Volume 97(6):1689-1696 March 15, 2001 ©2001 by American Society of Hematology The effect of detergents on CD36-associated proteins.(A) Platelet membrane proteins were labeled with biotin and lysed in 1% CHAPS (lane 1), Triton X-100 (lane 2), Brij 96 (lane 3), or Brij 99 (lane 4), and CD36 was immunoprecipitated with the monoclonal an... Wei-Min Miao et al. Blood 2001;97:1689-1696 ©2001 by American Society of Hematology Western blot analysis of CD36-associated proteins.Three detergents, CHAPS (lanes 1,2), Triton X-100 (lanes 3 and 4), and Brij 99 (lanes 5 and 6), were used to lyse the platelets. Wei-Min Miao et al. Blood 2001;97:1689-1696 ©2001 by American Society of Hematology Reciprocal immunoprecipitations.(A) The reciprocal immunoprecipitations with anti-CD9 (lane 2), anti-αIIb (lane 3), anti-β3 (lane 4), and anti-α6 (lane5) were compared to nonimmune mouse IgG (lane 6) and anti-CD36 antibody FA6-152 (lane 1). Wei-Min Miao et al. Blood 2001;97:1689-1696 ©2001 by American Society of Hematology Sucrose gradient ultracentrifugation of detergent extracts.(A) Biotinylated blood platelets were solubilized in 1% Triton X-100 (A, top) or Brij 99 (A, bottom) and centrifuged on 5% to 40% sucrose step gradients (see “Materials and methods”). Wei-Min Miao et al. Blood 2001;97:1689-1696 ©2001 by American Society of Hematology CD36-associated proteins on thrombasthenic platelets.Platelet membrane proteins from a patient with Glanzmann thrombasthenia were labeled with biotin and lysed in Brij 99 (lanes 1, 2, and 3) or CHAPS (lanes 4, 5, and 6). Wei-Min Miao et al. Blood 2001;97:1689-1696 ©2001 by American Society of Hematology Confocal immunofluorescence visualization of CD36 and α6 (A-D) or CD36 and CD9 (E-H) colocalization in human platelets.Fixed human platelets were simultaneously incubated with mouse anti-CD36 and rabbit anti-α6 antibodies (A-D) or rabbit anti-CD36 and mouse... Wei-Min Miao et al. Blood 2001;97:1689-1696 ©2001 by American Society of Hematology Confocal immunofluorescence visualization of CD36 and α6 (A-D) or CD9 and α6 (E-H) capping on human platelets.Fresh platelets were plated on fibronectin-coated coverslips and incubated with mouse anti-CD36 and rat anti-α6 antibodies (A-D) or mouse anti-CD9 ... Wei-Min Miao et al. Blood 2001;97:1689-1696 ©2001 by American Society of Hematology
