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Importation of Live SVC – Susceptible Fish species and Fertilized Eggs or Gametes – United States of America The consignment must comply with the following requirements as follows: a) the live SVC susceptible fish (or gametes) in this shipment originated from a country, zone, or aquaculture establishment (farm) that has been tested for Spring Viremia or Carp (SVC) twice a year (with at least 3 months before test) for a minimum of the previous two years; b) Water temperature at testing should be between 13-20°C, if applicable; c) Sample from fish less than one year of age (if applicable) should be collected by an official veterinarian of certifying official representating the competent authority, Malaysia and processed in a laboratory approved by the competent authority, Malaysia; d) The sample size should be provided for a 95% confidence level to detect an assumed 2% prevalence of infection in the source population; e) SVC virus detection asseys shall be included cell culture or other (specify)________; f) Cell culture shall be incubated on Ephithelioma papulosum cyprini (EPC) of Feathered minnow (FHM) cell lines at 15-25°C, with at least two serial passage and evaluation of cythopathic effect for a minimum of 7 days after each passage; and g) All testing result must be negative.