Download Quantitative phytochemical analysis and antioxidant

Quantitative phytochemical analysis and antioxidant activity from leaves
and twigs of Xylocarpus granatum
Siriporn Chewsuwan and Anan Athipornchai*
Department of Chemistry and Center of Excellence for Innovation in Chemistry,
Faculty of Science, Burapha University, Bangsaen, Chonburi 20131
*e-mail: [email protected]
Xylocarpus granatum is a species of mangrove in the mahogany family (Meliaceae).
This plant is used as a folk medicine for treating cholera, diarrhea, and fever diseases. In
this report, the free radical scavenging activity using DPPH assay, total phenolic and total
flavonoid contents of leaves and twigs from Xylocarpus granatum were studied.
Continuous Soxhlet extraction was used to prepare the leaf and twig extracts with seven
organic solvents. The percent yields of leaf extracts were higher than the twig extracts.
Moreover, the twig extracts showed higher total phenolic content (10.79 ± 0.52 to 174.81 ±
7.05 mgGAE/g) than leaf extracts (5.33 ± 0.25 to 37.70 ± 0.47 mgGAE/g) by using FolinCiocalteu assay while all extracts of this plants showed very low total flavonoid content by
using aluminium trichloride colorimetric assay. In addition, the twig extracts was also
more active (66.95 ± 0.14 to 98.44 ± 0.54%) than leaf extracts (43.62 ± 0.44 to 71.10 ±
0.48%) on DPPH inhibitory activity.
Keywords: Xylocarpus granatum; Total phenolic content; Total flavonoid content; DPPH scavenging
activity