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ABSTRACT Biological control is gaining prominence in the management of both soil borne and aerial plant diseases. Soil borne pathogens cause serious diseases in tomatoes. In nature microbial interaction involve competition, hyper parasitism, or antibiosis and these phenomena play an important role in striking ecological balance and keeping several plant pathogens in check. Biological control of plant pathogenic fungi has received considerable attention as it has several advantages such as possibility of multiple pathogen suppression, low cost and promotion of soil fertility. Three fungal pathogens were obtained from naturally infected plants. The aim of the study was to evaluate the ability of biocontrol agents in suppressing the growth of Alternaria species, the cause agent for early blight, Pythium species causing damping off of seedlings and Fusarium oxysporum that is responsible for Fusarium wilt. In vitro studies were carried out by employing the paired culture technique. Trichoderma sp and Bacillus subtilis inhibited the growth of all the three pathogens. Bacillus subtilis showed a higher percentage inhibition compared to Trichoderma sp. Biological control is highly recommended as it offers a more sustainable method for effective pest management. KEY WORDS: Biological control, Trichoderma sp, Bacillus subtilis, Pythium sp, Trichoderma sp, Alternaria sp, Tomato.