Download 14 unique colonies 9 distinct liquid media

wastewater
thyroid issues
breast cancer
urine
“superbugs”
stream biofilms
streams
soil
endocrine disruption
blood
benthic bacteria
cardiac muscle dysfunction
Understanding the
Environmental Implications
of Triclosan Degradation
Shivani S.R. Pandey
Mount Hebron High School
Johns Hopkins Applied Physics Lab
Dr. Charles Young
• Additional background
• Research focus and experiments
• Future plans
Outline
1
“down the drain”
consumer products
hospitals
food processing
2
3
wastewater
treatment plants
aquatic bodies
biodegradation
Chesapeake Bay
Atlantic Ocean
Into the Environment
14 unique colonies
9 distinct liquid media
tryptic soy broth (TSB)
minimal salts medium (MSM) + TCS
TSB + 5mg/L TCS
MSM + glucose
TSB + 50mg/L TCS
MSM + sodium acetate
TSB + 100mg/L TCS
MSM + TCS + glucose
MSM + TCS + sodium acetate
Experiments
Absorbance (nm)
1
8
15
22
29
36
43
50
57
64
71
78
85
92
99
106
113
120
127
134
141
148
155
162
169
176
183
190
197
204
211
218
225
232
239
246
253
260
1
0.1
50-2 in TCS + glucose
50-2 in TCS + sodium acetate
50-4 in TCS + glucose
50-11 in TCS + glucose
50-11 in TCS + sodium acetate
0.01
Results
Timepoints
• Toxic byproducts
• Mass spectrometry
• Degradation pathway
Future Research